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Literature summary extracted from

  • Singh, R.; Reynolds, K.A.
    Identification and characterization of FabA from the type II fatty acid synthase of Streptomyces coelicolor (2016), J. Nat. Prod., 79, 240-243 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.59 expression in Escherichia coli as soluble N-terminal His6-tag proteins Streptomyces coelicolor

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.59 Streptomyces coelicolor Q9L0M4 AND Q9L0M3 Q9L0M4: UPF0336 protein SCO4636, Q9L0M3: uncharacterized protein SCO4637
-
4.2.1.59 Streptomyces coelicolor ATCC BAA-471 Q9L0M4 AND Q9L0M3 Q9L0M4: UPF0336 protein SCO4636, Q9L0M3: uncharacterized protein SCO4637
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.59 3-hydroxydecanoyl-N-acetylcysteamine the N-acetylcysteamine group is structurally similar to the substrate binding end of the pantethine arm of acyl carrier proteins Streptomyces coelicolor ? + H2O
-
 ?
4.2.1.59 3-hydroxydecanoyl-N-acetylcysteamine the N-acetylcysteamine group is structurally similar to the substrate binding end of the pantethine arm of acyl carrier proteins Streptomyces coelicolor ATCC BAA-471 ? + H2O
-
 ?
4.2.1.59 3-hydroxyhexanoyl-N-acetylcysteamine the N-acetylcysteamine group is structurally similar to the substrate binding end of the pantethine arm of acyl carrier proteins Streptomyces coelicolor ? + H2O
-
 ?
4.2.1.59 3-hydroxyhexanoyl-N-acetylcysteamine the N-acetylcysteamine group is structurally similar to the substrate binding end of the pantethine arm of acyl carrier proteins Streptomyces coelicolor ATCC BAA-471 ? + H2O
-
 ?
4.2.1.59 3-hydroxyoctanoyl-N-acetylcysteamine the N-acetylcysteamine group is structurally similar to the substrate binding end of the pantethine arm of acyl carrier proteins Streptomyces coelicolor ? + H2O
-
 ?
4.2.1.59 3-hydroxyoctanoyl-N-acetylcysteamine the N-acetylcysteamine group is structurally similar to the substrate binding end of the pantethine arm of acyl carrier proteins Streptomyces coelicolor ATCC BAA-471 ? + H2O
-
 ?
4.2.1.59 5-methyl-3-hydroxyhexanoyl-N-acetylcysteamine the N-acetylcysteamine group is structurally similar to the substrate binding end of the pantethine arm of acyl carrier proteins Streptomyces coelicolor ? + H2O
-
 ?
4.2.1.59 5-methyl-3-hydroxyhexanoyl-N-acetylcysteamine the N-acetylcysteamine group is structurally similar to the substrate binding end of the pantethine arm of acyl carrier proteins Streptomyces coelicolor ATCC BAA-471 ? + H2O
-
 ?
4.2.1.59 6-methyl-3-hydroxyheptanoyl-N-acetylcysteamine the N-acetylcysteamine group is structurally similar to the substrate binding end of the pantethine arm of acyl carrier proteins Streptomyces coelicolor ? + H2O
-
 ?
4.2.1.59 6-methyl-3-hydroxyheptanoyl-N-acetylcysteamine the N-acetylcysteamine group is structurally similar to the substrate binding end of the pantethine arm of acyl carrier proteins Streptomyces coelicolor ATCC BAA-471 ? + H2O
-
 ?
4.2.1.59 7-methyl-3-hydroxyoctanoyl-N-acetylcysteamine the N-acetylcysteamine group is structurally similar to the substrate binding end of the pantethine arm of acyl carrier proteins Streptomyces coelicolor ? + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.59 heterodimer 1 * 15000 + 1 * 20000, SDS-PAGE Streptomyces coelicolor

Synonyms

EC Number Synonyms Comment Organism
4.2.1.59 3-hydroxyacyl-ACP dehydratase
-
 Streptomyces coelicolor
4.2.1.59 FabA
-
 Streptomyces coelicolor

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.59 25
-
 assay at Streptomyces coelicolor

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.59 7.2
-
 assay at Streptomyces coelicolor

General Information

EC Number General Information Comment Organism
4.2.1.59 metabolism the enzyme also processes intermediates in both fatty acid and undecylprodiginine biosynthetic processes. It is capable of utilizing various chain lengths of straight- and branched-chain 3-hydroxyacyl-N-acetyl cysteamine substrates. FabA does not discriminate between acyl carrier proteins from primary and secondary metabolism. It processes intermediates for both biosynthetic pathways Streptomyces coelicolor

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.1.59 144
-
 7-methyl-3-hydroxyoctanoyl-N-acetylcysteamine pH 7.2, 25°C Streptomyces coelicolor
4.2.1.59 226
-
 3-hydroxydecanoyl-N-acetylcysteamine pH 7.2, 25°C Streptomyces coelicolor
4.2.1.59 363
-
 3-hydroxyoctanoyl-N-acetylcysteamine pH 7.2, 25°C Streptomyces coelicolor
4.2.1.59 484
-
 3-hydroxyhexanoyl-N-acetylcysteamine pH 7.2, 25°C Streptomyces coelicolor
4.2.1.59 522
-
 5-methyl-3-hydroxyhexanoyl-N-acetylcysteamine pH 7.2, 25°C Streptomyces coelicolor
4.2.1.59 527
-
 6-methyl-3-hydroxyheptanoyl-N-acetylcysteamine pH 7.2, 25°C Streptomyces coelicolor