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Literature summary extracted from

  • Lee, K.W.; Shim, J.M.; Yao, Z.; Kim, J.A.; Kim, H.J.; Kim, J.H.
    Characterization of a glutamate decarboxylase (GAD) from Enterococcus avium M5 isolated from Jeotgal, a Korean fermented seafood (2017), J. Microbiol. Biotechnol., 27, 1216-1222 .
    View publication on PubMed

Application

EC Number Application Comment Organism
4.1.1.15 food industry Enterococcus avium strain M5 and its gadB gene might be useful when GABA-enriched foods are attempted to be produced, serving as a source of strain and gene Enterococcus avium

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.15 gene gadB, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Enterococcus avium

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.15 AgNO3 44.4% inhibition at 2 mM Enterococcus avium
4.1.1.15 CuSO4 56.8% inhibition at 2 mM Enterococcus avium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.15 3.26
-
 L-glutamate pH 5.5, 45°C, recombinant His-tagged enzyme Enterococcus avium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.15 CaCl2 activates to 124.3% activity at 2 mM Enterococcus avium
4.1.1.15 MnCl2 activates to 116.4% activity at 2 mM Enterococcus avium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.15 L-glutamate Enterococcus avium
-
 4-aminobutanoate + CO2
-
 ?
4.1.1.15 L-glutamate Enterococcus avium M5
-
 4-aminobutanoate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.15 Enterococcus avium E0D5D7 isolated from Jeot-gal, a Korean fermented seafood
-
4.1.1.15 Enterococcus avium M5 E0D5D7 isolated from Jeot-gal, a Korean fermented seafood
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.15 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Enterococcus avium

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.1.15 additional information strain M5 produces 18.47 mg/ml GABA when incubated for 48 h at 37°C in MRS broth with 3% w/v monosodium L-glutamate. Strain M5 secretes a lot of GABA when grown on MRS medium with monosodium L-glutamate Enterococcus avium
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.15 additional information
-
 strain M5 produces 18.47 mg/ml GABA when incubated for 48 h at 37°C in MRS broth with 3% w/v monosodium L-glutamate Enterococcus avium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.15 L-glutamate
-
 Enterococcus avium 4-aminobutanoate + CO2
-
 ?
4.1.1.15 L-glutamate
-
 Enterococcus avium M5 4-aminobutanoate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.15 ? x * 53755, sequence calculation, x * 53000, recombinant His-tagged enzyme, SDS-PAGE Enterococcus avium

Synonyms

EC Number Synonyms Comment Organism
4.1.1.15 GAD
-
 Enterococcus avium
4.1.1.15 GadB
-
 Enterococcus avium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.15 55
-
 recombinant His-tagged enzyme Enterococcus avium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.15 4.5
-
 recombinant His-tagged enzyme Enterococcus avium

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.15 pyridoxal 5'-phosphate dependent on, inactive without Enterococcus avium

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.1.1.15 Enterococcus avium sequence calculation
-
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