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Literature summary extracted from

  • Yang, S.; Lin, Q.; Lu, Z.-X.; Lu, F.-X.; Bie, X.-M.; Zou, X.-K.; Sun, L.-J.
    Characterization of a novel glutamate decarboxylase from Streptococcus salivarius ssp. thermophilus Y2 (2008), J. Chem. Technol. Biotechnol., 83, 855-861 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.15 gene gadB, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in Escherichia coli strain BL21(DE3) Streptococcus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.15 AgNO3 complete inhibition at 5 mM Streptococcus thermophilus
4.1.1.15 CaCl2 95.4% remaining activity at 5 mM Streptococcus thermophilus
4.1.1.15 CoCl2 37.9% remaining activity at 5 mM; 62.1% inhibition at 5 mM Streptococcus thermophilus
4.1.1.15 CuSO4 92.4% inhibition at 5 mM Streptococcus thermophilus
4.1.1.15 FeSO4 91.9% inhibition at 5 mM Streptococcus thermophilus
4.1.1.15 KCl 5.8% inhibition at 5 mM; 94.2% remaining activity at 5 mM Streptococcus thermophilus
4.1.1.15 LiCl 6.1% inhibition at 5 mM; 93.9% remaining activity at 5 mM Streptococcus thermophilus
4.1.1.15 MnSO4 87.9% inhibition at 5 mM Streptococcus thermophilus
4.1.1.15 Na2SO4 9.8% inhibition at 5 mM Streptococcus thermophilus
4.1.1.15 ZnSO4 80.4% inhibition at 5 mM Streptococcus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.15 additional information
-
 additional information Lineweaver-Burk plots Streptococcus thermophilus
4.1.1.15 2.3
-
 L-glutamate pH 4.0, 55°C Streptococcus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.15 BaCl2 activates to 122.5% at 5 mM Streptococcus thermophilus
4.1.1.15 MgCl2 98.7% of control activity at 5 mM Streptococcus thermophilus
4.1.1.15 NaCl 101.6% of control activity at 5 mM Streptococcus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.15 103570
-
 gel filtration Streptococcus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.15 L-glutamate Streptococcus thermophilus
-
 4-aminobutanoate + CO2
-
 ir
4.1.1.15 L-glutamate Streptococcus thermophilus DSM 20617
-
 4-aminobutanoate + CO2
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.15 Streptococcus thermophilus Q0GE18 i.e. Streptococcus thermophilus
-
4.1.1.15 Streptococcus thermophilus DSM 20617 Q0GE18 i.e. Streptococcus thermophilus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.15 native enzyme GAD 22fold by ammonium sulfate fractionation, dialysis, precipitation in N-methylpiperazine, anion exchange and hydrophobic interaction chromatography, and gel filtration Streptococcus thermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.15 103.5
-
 purified native enzyme, pH 4.0, 55°C Streptococcus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.15 L-glutamate
-
 Streptococcus thermophilus 4-aminobutanoate + CO2
-
 ir
4.1.1.15 L-glutamate irreversible alpha-decarboxylation of L-glutamate Streptococcus thermophilus 4-aminobutanoate + CO2
-
 ir
4.1.1.15 L-glutamate
-
 Streptococcus thermophilus DSM 20617 4-aminobutanoate + CO2
-
 ir
4.1.1.15 L-glutamate irreversible alpha-decarboxylation of L-glutamate Streptococcus thermophilus DSM 20617 4-aminobutanoate + CO2
-
 ir
4.1.1.15 additional information the enzyme is highly substrate specific. No activity with D-glutamate and no activity with L-Asp, L-Ser, L-His, Gly, L-Thr, L-Ala, L-Arg, L-Tyr, L-Cys, L-Val, L-Met, L-Trp, L-Phe, L-Ile, L-Leu, L-Lys, and L-Pro Streptococcus thermophilus ?
-
 ?
4.1.1.15 additional information the enzyme is highly substrate specific. No activity with D-glutamate and no activity with L-Asp, L-Ser, L-His, Gly, L-Thr, L-Ala, L-Arg, L-Tyr, L-Cys, L-Val, L-Met, L-Trp, L-Phe, L-Ile, L-Leu, L-Lys, and L-Pro Streptococcus thermophilus DSM 20617 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.15 homodimer 2 * 46850, SDS-PAGE Streptococcus thermophilus
4.1.1.15 More the N-terminal amino acid sequence of GAD is NH2-MNEKLFREI Streptococcus thermophilus

Synonyms

EC Number Synonyms Comment Organism
4.1.1.15 GAD
-
 Streptococcus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.15 55
-
-
 Streptococcus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.15 4
-
-
 Streptococcus thermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.15 pyridoxal 5'-phosphate
-
 Streptococcus thermophilus

General Information

EC Number General Information Comment Organism
4.1.1.15 physiological function gamma-aminobutyric acid with several physiological functions is biosynthesized via the irreversible alpha-decarboxylation of L-glutamate catalysed by glutamate decarboxylase (GAD). Streptococcus salivarius ssp. thermophilus is widely applied to the dairy Streptococcus thermophilus