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Literature summary extracted from

  • Zhang, Z.; Akutsu, J.; Tsujimura, M.; Kawarabayasi, Y.
    Increasing in archaeal GlcNAc-1-P uridyltransferase activity by targeted mutagenesis while retaining its extreme thermostability (2007), J. Biochem., 141, 553-562 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.23 expression in Escherichia coli Sulfurisphaera tokodaii
2.7.7.24 expression in Escherichia coli Sulfurisphaera tokodaii

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.23 D208A GlcNAc-1-P UTase activity is 10% of the activity of wild-type ST0452 protein Sulfurisphaera tokodaii
2.7.7.23 D99A no GlcNAc-1-P UTase activity Sulfurisphaera tokodaii
2.7.7.23 E146A no GlcNAc-1-P UTase activity Sulfurisphaera tokodaii
2.7.7.23 G9A GlcNAc-1-P UTase activity is less than 5% Sulfurisphaera tokodaii
2.7.7.23 K147A GlcNAc-1-P UTase activity is 25% of the activity of wild-type ST0452 protein Sulfurisphaera tokodaii
2.7.7.23 K23A no GlcNAc-1-P UTase activity Sulfurisphaera tokodaii
2.7.7.23 R13A GlcNAc-1-P UTase activity is 10% of the activity of wild-type ST0452 protein Sulfurisphaera tokodaii
2.7.7.23 T80A GlcNAc-1-P UTase activity is 56% of the activity of wild-type ST0452 protein Sulfurisphaera tokodaii
2.7.7.23 T80L no GlcNAc-1-P UTase activity Sulfurisphaera tokodaii
2.7.7.23 Y97A GlcNAc-1-P UTase activity is 66% of the activity of wild-type ST0452 protein Sulfurisphaera tokodaii
2.7.7.23 Y97F GlcNAc-1-P UTase activity is similar to wild-type ST0452 protein Sulfurisphaera tokodaii
2.7.7.24 D208A very low Glc-1-P TTase activity Sulfurisphaera tokodaii
2.7.7.24 D99A very low Glc-1-P TTase activity Sulfurisphaera tokodaii
2.7.7.24 E146A very low Glc-1-P TTase activity Sulfurisphaera tokodaii
2.7.7.24 G9A very low Glc-1-P TTase activity Sulfurisphaera tokodaii
2.7.7.24 K147A very low Glc-1-P TTase activity Sulfurisphaera tokodaii
2.7.7.24 K23A very low Glc-1-P TTase activity Sulfurisphaera tokodaii
2.7.7.24 R13A very low Glc-1-P TTase activity Sulfurisphaera tokodaii
2.7.7.24 T80A Glc-1-P TTase activity is about 1.5fold higher than that of wild-type ST0452 protein Sulfurisphaera tokodaii
2.7.7.24 T80L very low Glc-1-P TTase activity Sulfurisphaera tokodaii
2.7.7.24 Y97A Glc-1-P TTase activity is about 2.2fold higher than that of wild-type ST0452 protein Sulfurisphaera tokodaii
2.7.7.24 Y97A very low Glc-1-P TTase activity Sulfurisphaera tokodaii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.23 0.0017
-
 UTP pH 7.5, 80°C Sulfurisphaera tokodaii
2.7.7.23 0.008
-
 N-acetyl-alpha-D-glucosamine 1-phosphate pH 7.5, 80°C Sulfurisphaera tokodaii
2.7.7.23 0.016
-
 diphosphate pH 7.5, 80°C Sulfurisphaera tokodaii
2.7.7.23 0.016
-
 UDP-N-acetyl-alpha-D-glucosamine pH 7.5, 80°C Sulfurisphaera tokodaii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.23 Ca2+ no activity is detectable when a divalent cation is removed from the reaction buffer. The order of effectiveness of metal ions on GlcNAc-1-P UTase activity is Mg2+ > Zn2+ > Ca2+ > Co2+ > Mn2+, which is different from that of its Glc-1-P TTase activity, Co2+ > Mn2+ > Mg2+ > Zn2+ > Ca2+ Sulfurisphaera tokodaii
2.7.7.23 Co2+ no activity is detectable when a divalent cation is removed from the reaction buffer. The order of effectiveness of metal ions on GlcNAc-1-P UTase activity is Mg2+ > Zn2+ > Ca2+ > Co2+ > Mn2+, which is different from that of its Glc-1-P TTase activity, Co2+ > Mn2+ > Mg2+ > Zn2+ > Ca2+ Sulfurisphaera tokodaii
2.7.7.23 Mg2+ no activity is detectable when a divalent cation is removed from the reaction buffer. The order of effectiveness of metal ions on GlcNAc-1-P UTase activity is Mg2+ > Zn2+ > Ca2+ > Co2+ > Mn2+, which is different from that of its Glc-1-P TTase activity, Co2+ > Mn2+ > Mg2+ > Zn2+ > Ca2+ Sulfurisphaera tokodaii
2.7.7.23 Mn2+ no activity is detectable when a divalent cation is removed from the reaction buffer. The order of effectiveness of metal ions on GlcNAc-1-P UTase activity is Mg2+ > Zn2+ > Ca2+ > Co2+ > Mn2+, which is different from that of its Glc-1-P TTase activity, Co2+ > Mn2+ > Mg2+ > Zn2+ > Ca2+ Sulfurisphaera tokodaii
2.7.7.23 Zn2+ no activity is detectable when a divalent cation is removed from the reaction buffer. The order of effectiveness of metal ions on GlcNAc-1-P UTase activity is Mg2+ > Zn2+ > Ca2+ > Co2+ > Mn2+, which is different from that of its Glc-1-P TTase activity, Co2+ > Mn2+ > Mg2+ > Zn2+ > Ca2+ Sulfurisphaera tokodaii
2.7.7.24 Ca2+ no activity is detectable when a divalent cation is removed from the reaction buffer. The order of effectiveness of metal ions on GlcNAc-1-P UTase activity is Mg2+ > Zn2+ > Ca2+ > Co2+ > Mn2+, which is different from that of its Glc-1-P TTase activity, Co2+ > Mn2+ > Mg2+ > Zn2+ > Ca2+ Sulfurisphaera tokodaii
2.7.7.24 Co2+ no activity is detectable when a divalent cation is removed from the reaction buffer. The order of effectiveness of metal ions on GlcNAc-1-P UTase activity is Mg2+ > Zn2+ > Ca2+ > Co2+ > Mn2+, which is different from that of its Glc-1-P TTase activity, Co2+ > Mn2+ > Mg2+ > Zn2+ > Ca2+ Sulfurisphaera tokodaii
2.7.7.24 Mg2+ no activity is detectable when a divalent cation is removed from the reaction buffer. The order of effectiveness of metal ions on GlcNAc-1-P UTase activity is Mg2+ > Zn2+ > Ca2+ > Co2+ > Mn2+, which is different from that of its Glc-1-P TTase activity, Co2+ > Mn2+ > Mg2+ > Zn2+ > Ca2+ Sulfurisphaera tokodaii
2.7.7.24 Mn2+ no activity is detectable when a divalent cation is removed from the reaction buffer. The order of effectiveness of metal ions on GlcNAc-1-P UTase activity is Mg2+ > Zn2+ > Ca2+ > Co2+ > Mn2+, which is different from that of its Glc-1-P TTase activity, Co2+ > Mn2+ > Mg2+ > Zn2+ > Ca2+ Sulfurisphaera tokodaii
2.7.7.24 Zn2+ no activity is detectable when a divalent cation is removed from the reaction buffer. The order of effectiveness of metal ions on GlcNAc-1-P UTase activity is Mg2+ > Zn2+ > Ca2+ > Co2+ > Mn2+, which is different from that of its Glc-1-P TTase activity, Co2+ > Mn2+ > Mg2+ > Zn2+ > Ca2+ Sulfurisphaera tokodaii

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.23 Sulfurisphaera tokodaii Q975F9
-
-
2.7.7.23 Sulfurisphaera tokodaii DSM 16993 Q975F9
-
-
2.7.7.24 Sulfurisphaera tokodaii Q975F9
-
-
2.7.7.24 Sulfurisphaera tokodaii DSM 16993 Q975F9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.23
-
 Sulfurisphaera tokodaii
2.7.7.24
-
 Sulfurisphaera tokodaii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.23 diphosphate + UDP-N-acetyl-alpha-D-glucosamine
-
 Sulfurisphaera tokodaii UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r
2.7.7.23 diphosphate + UDP-N-acetyl-alpha-D-glucosamine
-
 Sulfurisphaera tokodaii DSM 16993 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r
2.7.7.23 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 Sulfurisphaera tokodaii diphosphate + UDP-N-acetyl-alpha-D-glucosamine
-
 r
2.7.7.23 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 Sulfurisphaera tokodaii DSM 16993 diphosphate + UDP-N-acetyl-alpha-D-glucosamine
-
 r
2.7.7.24 dTTP + alpha-D-glucose 1-phosphate
-
 Sulfurisphaera tokodaii diphosphate + dTDP-alpha-D-glucose
-
 ?
2.7.7.24 dTTP + alpha-D-glucose 1-phosphate
-
 Sulfurisphaera tokodaii DSM 16993 diphosphate + dTDP-alpha-D-glucose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.23 GlcNAc-1-P uridyltransferase
-
 Sulfurisphaera tokodaii
2.7.7.23 GlcNAc-1-P UTase
-
 Sulfurisphaera tokodaii
2.7.7.23 ST0452
-
 Sulfurisphaera tokodaii
2.7.7.24 Glc-1-P TTase
-
 Sulfurisphaera tokodaii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.23 80
-
 assay at Sulfurisphaera tokodaii
2.7.7.24 80
-
 assay at Sulfurisphaera tokodaii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.23 2.54
-
 N-acetyl-alpha-D-glucosamine 1-phosphate pH 7.5, 80°C Sulfurisphaera tokodaii
2.7.7.23 3.53
-
 UTP pH 7.5, 80°C Sulfurisphaera tokodaii
2.7.7.23 6.21
-
 diphosphate pH 7.5, 80°C Sulfurisphaera tokodaii
2.7.7.23 8.4
-
 UDP-N-acetyl-alpha-D-glucosamine pH 7.5, 80°C Sulfurisphaera tokodaii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.23 7.5
-
 assay at Sulfurisphaera tokodaii
2.7.7.24 7.5
-
 assay at Sulfurisphaera tokodaii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.7.23 320
-
 N-acetyl-alpha-D-glucosamine 1-phosphate pH 7.5, 80°C Sulfurisphaera tokodaii
2.7.7.23 380
-
 diphosphate pH 7.5, 80°C Sulfurisphaera tokodaii
2.7.7.23 530
-
 UDP-N-acetyl-alpha-D-glucosamine pH 7.5, 80°C Sulfurisphaera tokodaii
2.7.7.23 2120
-
 UTP pH 7.5, 80°C Sulfurisphaera tokodaii