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Literature summary extracted from

  • Khan, M.; Akbar, M.; Kitaoka, M.; Hayashi, K.
    A unique thermostable lichenase from Thermotoga maritima MSB8 with divergent substrate specificity (2007), Indian J. Biotechnol., 6, 315-320 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.73 expression in Escherichia coli Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.73 additional information
-
 Barley beta-glucan Km: 0.67 mg/ml, pH 6.6, 70°C Thermotoga maritima
3.2.1.73 additional information
-
 lichenan Km: 0.78 mg/ml, pH 6.6, 70°C Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.73 Thermotoga maritima Q9X274
-
-
3.2.1.73 Thermotoga maritima DSM 3109 Q9X274
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.73 barley beta-glucan + H2O
-
 Thermotoga maritima ?
-
 ?
3.2.1.73 barley beta-glucan + H2O
-
 Thermotoga maritima DSM 3109 ?
-
 ?
3.2.1.73 lichenan + H2O beta-D-Glc-(1->4)-beta-D-Glc-(1->3)-beta-D-Glc is not detected as product Thermotoga maritima beta-D-Glc-(1->3)-beta-D-Glc-(1->4)-beta-D-Glc + ?
-
 ?
3.2.1.73 lichenan + H2O beta-D-Glc-(1->4)-beta-D-Glc-(1->3)-beta-D-Glc is not detected as product Thermotoga maritima DSM 3109 beta-D-Glc-(1->3)-beta-D-Glc-(1->4)-beta-D-Glc + ?
-
 ?
3.2.1.73 additional information no activity with crystalline cellulose, carbocymethyl cellulose, Avicel, curdlan, starch, pustulan, birchwood xylan or laminarin Thermotoga maritima ?
-
 ?
3.2.1.73 additional information no activity with crystalline cellulose, carbocymethyl cellulose, Avicel, curdlan, starch, pustulan, birchwood xylan or laminarin Thermotoga maritima DSM 3109 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.73 ? x * 39960, SDS-PAGE Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
3.2.1.73 Lichenase
-
 Thermotoga maritima
3.2.1.73 TM1752
-
 Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.73 70
-
-
 Thermotoga maritima

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.73 6.2
-
 Barley beta-glucan pH 6.6, 70°C Thermotoga maritima
3.2.1.73 7
-
 lichenan pH 6.6, 70°C Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.73 6.6
-
-
 Thermotoga maritima