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Literature summary extracted from

  • Valkonen, M.; Penttilae, M.; Bencina, M.
    Intracellular pH responses in the industrially important fungus Trichoderma reesei (2014), Fungal Genet. Biol., 70, 86-93 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.146 gene lgd1, expression analysis in wild-type and mutant strain, introduction of pHluorin, called RaVC, for the analysis of intracellular pH, the RaVC gene is cloned into expression vector pMV119 using yeast recombinational cloning, and cytosolic localization Trichoderma reesei

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.146 additional information strain M106, derived from wild-type strain Rut-C30, accumulates L-galactonic acid from provided galacturonic acid due to a L-galactonate dehydratase deletion. Strain M106 maintains higher intracellular pH in the presence of D-galacturonic acid than its parental strain Rut-C30. The acidic external pH caused significant acidification of cytosol Trichoderma reesei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.146 L-galactonate Trichoderma reesei
-
 2-dehydro-3-deoxy-L-galactonate + H2O
-
 ?
4.2.1.146 L-galactonate Trichoderma reesei Rut-C30
-
 2-dehydro-3-deoxy-L-galactonate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.146 Trichoderma reesei Q0QWS4 i.e. Hypocrea jecorina
-
4.2.1.146 Trichoderma reesei Rut-C30 Q0QWS4 i.e. Hypocrea jecorina
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.146 L-galactonate
-
 Trichoderma reesei 2-dehydro-3-deoxy-L-galactonate + H2O
-
 ?
4.2.1.146 L-galactonate
-
 Trichoderma reesei Rut-C30 2-dehydro-3-deoxy-L-galactonate + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.146 LGD1
-
 Trichoderma reesei