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Literature summary extracted from

  • Motojima, F.; Nuylert, A.; Asano, Y.
    The crystal structure and catalytic mechanism of hydroxynitrile lyase from passion fruit, Passiflora edulis (2018), FEBS J., 285, 313-324 .
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.2.10 crystals of the His6-tagged hydroxynitrile lyase are prepared using vapor diffusion sitting drop method at 20°C. The crystal structures of Passiflora edulis hydroxynitrile lyase and its C-terminal peptide depleted derivative are determined by molecular replacement method using the template structure of a heat stable protein, SP1, from Populus tremula at 2.8 and 1.8 A resolution, respectively. Passiflora edulis hydroxynitrile lyase belongs to dimeric alpha+beta barrel superfamily consisting of a central beta-barrel in the middle of a dimer. The structure of Passiflora edulis hydroxynitrile lyase complexed with (R)-mandelonitrile is also determined. The hydroxyl group of (R)-mandelonitrile forms hydrogen bonds with His8 and Tyr30 in the active site, whereas the nitrile group is oriented toward the carboxyl group of Glu54, unlike other hydroxynitrile lyases, where it interacts with basic residues typically Passiflora edulis

Protein Variants

EC Number Protein Variants Comment Organism
4.1.2.10 E54A completely inactive mutant enzyme Passiflora edulis
4.1.2.10 E54Q mutant enzyme exhibits about 8 of wild-type activity Passiflora edulis
4.1.2.10 H8A completely inactive mutant enzyme Passiflora edulis
4.1.2.10 N101A completely inactive mutant enzyme Passiflora edulis
4.1.2.10 N101D completely inactive mutant enzyme Passiflora edulis
4.1.2.10 R56A mutant enzyme exhibits about 5% of wild-type activity Passiflora edulis
4.1.2.10 S66A mutant enzyme exhibits about 10p% of wild-type activity Passiflora edulis
4.1.2.10 Y30F mutant enzyme exhibits about 5% of wild-type activity Passiflora edulis

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.10 Passiflora edulis A0A1L7NZN4
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.10 (R)-mandelonitrile
-
 Passiflora edulis cyanide + benzaldehyde
-
 r
4.1.2.10 cyanide + benzaldehyde
-
 Passiflora edulis (R)-mandelonitrile
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.2.10 25
-
 assay at Passiflora edulis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.10 4.5
-
 assay at Passiflora edulis