BRENDA - Enzyme Database

Characterization of a quinole-oxidase activity in crude extracts of Thermoplasma acidophilum and isolation of an 18-kDa cytochrome

Gartner, P.; Eur. J. Biochem. 200, 215-222 (1991) View publication on PubMed

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
1.10.3.12
addition of total lipids from Thermoplasma acidophilum increases stability
Thermoplasma acidophilum
1.10.3.12
asolectin from soya beans decreases stability
Thermoplasma acidophilum
1.10.3.12
stability is lowered by the presence of detergent
Thermoplasma acidophilum
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.10.3.12
2-hydroxy-3-undecyl-1,4-naphthoquinone
0.01 mM, 20% inhibition
Thermoplasma acidophilum
1.10.3.12
2n-heptyl-4-hydroxyquinoline N-oxide
most potent inhibitor
Thermoplasma acidophilum
1.10.3.12
asolectin
-
Thermoplasma acidophilum
1.10.3.12
Lipid
addition of total lipids from Thermoplasma acidophilum decreases activity
Thermoplasma acidophilum
1.10.3.12
additional information
antimycin A, myxothiazol, methyl 3-methoxy-2-(2-styrylphenyl) propenate and stigmatellin (at concentration up to 0.030 mM) and Zn2+ at a concentration up to 1 mM, do not inhibit
Thermoplasma acidophilum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.10.3.12
0.018
-
ubiquinol-10
pH 5.4, 50°C
Thermoplasma acidophilum
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.10.3.12
membrane
-
Thermoplasma acidophilum
16020
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.10.3.12
150000
-
gel filtration
Thermoplasma acidophilum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.10.3.12
thermoplasmaquinol + O2
Thermoplasma acidophilum
likely candidate to the physiological electron donor
thermoplasmaquinone + H2O
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.10.3.12
Thermoplasma acidophilum
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.10.3.12
-
Thermoplasma acidophilum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.10.3.12
2,3-dimethylnaphthohydroquinone + O2
-
747686
Thermoplasma acidophilum
?
-
-
-
?
1.10.3.12
menadione + O2
-
747686
Thermoplasma acidophilum
?
-
-
-
?
1.10.3.12
menaquinol-4 + O2
menaquinols are oxidized at 6-12fold higher rates than ubiquinols
747686
Thermoplasma acidophilum
menaquinone-4 + H2O
-
-
-
?
1.10.3.12
thermoplasmaquinol + O2
likely candidate to the physiological electron donor
747686
Thermoplasma acidophilum
thermoplasmaquinone + H2O
-
-
-
?
1.10.3.12
ubiquinol-1 + O2
-
747686
Thermoplasma acidophilum
ubiquinone-1 + H2O
-
-
-
?
1.10.3.12
ubiquinol-10 + O2
menaquinols are oxidized at 6-12fold higher rates than ubiquinols. However, because of the greater tendency for autoxidation of menaquinols, ubiquinol is a more convenient substrate for the enzyme assay
747686
Thermoplasma acidophilum
ubiquinone-10 + H2O
-
-
-
?
1.10.3.12
ubiquinol-6 + O2
-
747686
Thermoplasma acidophilum
ubiquinone-6 + H2O
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.10.3.12
oligomer
12-16 * 14000, SDS-PAGE
Thermoplasma acidophilum
Synonyms
EC Number
Synonyms
Commentary
Organism
1.10.3.12
Q10H2 oxidase
-
Thermoplasma acidophilum
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.10.3.12
50
-
-
Thermoplasma acidophilum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.10.3.12
5.4
-
-
Thermoplasma acidophilum
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.10.3.12
cytochrome
cytochrome-o-type quinol oxidase
Thermoplasma acidophilum
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.10.3.12
0.00015
-
2n-heptyl-4-hydroxyquinoline N-oxide
pH 5.4, 50°C
Thermoplasma acidophilum
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.10.3.12
cytochrome
cytochrome-o-type quinol oxidase
Thermoplasma acidophilum
General Stability (protein specific)
EC Number
General Stability
Organism
1.10.3.12
addition of total lipids from Thermoplasma acidophilum increases stability
Thermoplasma acidophilum
1.10.3.12
asolectin from soya beans decreases stability
Thermoplasma acidophilum
1.10.3.12
stability is lowered by the presence of detergent
Thermoplasma acidophilum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.10.3.12
2-hydroxy-3-undecyl-1,4-naphthoquinone
0.01 mM, 20% inhibition
Thermoplasma acidophilum
1.10.3.12
2n-heptyl-4-hydroxyquinoline N-oxide
most potent inhibitor
Thermoplasma acidophilum
1.10.3.12
asolectin
-
Thermoplasma acidophilum
1.10.3.12
Lipid
addition of total lipids from Thermoplasma acidophilum decreases activity
Thermoplasma acidophilum
1.10.3.12
additional information
antimycin A, myxothiazol, methyl 3-methoxy-2-(2-styrylphenyl) propenate and stigmatellin (at concentration up to 0.030 mM) and Zn2+ at a concentration up to 1 mM, do not inhibit
Thermoplasma acidophilum
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.10.3.12
0.00015
-
2n-heptyl-4-hydroxyquinoline N-oxide
pH 5.4, 50°C
Thermoplasma acidophilum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.10.3.12
0.018
-
ubiquinol-10
pH 5.4, 50°C
Thermoplasma acidophilum
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.10.3.12
membrane
-
Thermoplasma acidophilum
16020
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.10.3.12
150000
-
gel filtration
Thermoplasma acidophilum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.10.3.12
thermoplasmaquinol + O2
Thermoplasma acidophilum
likely candidate to the physiological electron donor
thermoplasmaquinone + H2O
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.10.3.12
-
Thermoplasma acidophilum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.10.3.12
2,3-dimethylnaphthohydroquinone + O2
-
747686
Thermoplasma acidophilum
?
-
-
-
?
1.10.3.12
menadione + O2
-
747686
Thermoplasma acidophilum
?
-
-
-
?
1.10.3.12
menaquinol-4 + O2
menaquinols are oxidized at 6-12fold higher rates than ubiquinols
747686
Thermoplasma acidophilum
menaquinone-4 + H2O
-
-
-
?
1.10.3.12
thermoplasmaquinol + O2
likely candidate to the physiological electron donor
747686
Thermoplasma acidophilum
thermoplasmaquinone + H2O
-
-
-
?
1.10.3.12
ubiquinol-1 + O2
-
747686
Thermoplasma acidophilum
ubiquinone-1 + H2O
-
-
-
?
1.10.3.12
ubiquinol-10 + O2
menaquinols are oxidized at 6-12fold higher rates than ubiquinols. However, because of the greater tendency for autoxidation of menaquinols, ubiquinol is a more convenient substrate for the enzyme assay
747686
Thermoplasma acidophilum
ubiquinone-10 + H2O
-
-
-
?
1.10.3.12
ubiquinol-6 + O2
-
747686
Thermoplasma acidophilum
ubiquinone-6 + H2O
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.10.3.12
oligomer
12-16 * 14000, SDS-PAGE
Thermoplasma acidophilum
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.10.3.12
50
-
-
Thermoplasma acidophilum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.10.3.12
5.4
-
-
Thermoplasma acidophilum