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Literature summary extracted from

  • Lanfranchi, E.; Grill, B.; Raghoebar, Z.; Van Pelt, S.; Sheldon, R.A.; Steiner, K.; Glieder, A.; Winkler, M.
    Production of hydroxynitrile Lyase from Davallia tyermannii (DtHNL) in Komagataella phaffii and its immobilization as a CLEA to generate a robust biocatalyst (2018), ChemBioChem, 19, 312-316 .
    View publication on PubMed

Application

EC Number Application Comment Organism
4.1.2.10 synthesis the enzyme may find application as a biocatalyst for the synthesis of enantiomerically pure cyanohydrins. DtHNL1-CLEA preparation results in a more robust biocatalyst under acidic conditions, which is an interesting feature for the efficient production of enantiomerically pure cyanohydrins Davallia tyermanii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.10 expression in Escherichia coli. Recombinant production of DtHNL1 in Komagataella phaffii under control of the strong and tightly regulated AOX1 promoter. Two different shuttle vectors are used, pPpT4_S and pPpB1, to obtain strains with optimal gene copy numbers for efficient heterologous expression Davallia tyermanii

General Stability

EC Number General Stability Organism
4.1.2.10 crosslinked enzyme aggregates result in significant enhancement of the biocatalyst stability under acidic conditions (activity is retained after 168 h at pH 2.4) Davallia tyermanii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.10 20000
-
 SDS-PAGE Davallia tyermanii

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.10 Davallia tyermanii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.10
-
 Davallia tyermanii

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.10 (R)-mandelonitrile
-
 Davallia tyermanii cyanide + benzaldehyde
-
 r
4.1.2.10 cyanide + benzaldehyde
-
 Davallia tyermanii (R)-mandelonitrile
-
 r
4.1.2.10 cyanide + hydroxypivaldehyde crosslinked enzyme aggregates catalyze synthesis of (R)-hydroxypivaldehyde cyanohydrin under reaction conditions that immediately inactivated non-immobilized enzyme Davallia tyermanii (R)-hydroxypivaldehyde cyanohydrin
-
 r

Subunits

EC Number Subunits Comment Organism
4.1.2.10 ? x * 20000, SDS-PAGE Davallia tyermanii

Synonyms

EC Number Synonyms Comment Organism
4.1.2.10 DtHNL1
-
 Davallia tyermanii