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Literature summary extracted from

  • Liu, Z.; Jin, Y.; Tao, Y.; Wang, G.
    Crystal structure of IspF from Bacillus subtilis and absence of protein complex assembly amongst IspD/IspE/IspF enzymes in the MEP pathway (2018), Biosci. Rep., 38, BSR20171370 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.148 gene ispE, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) from pGEX-6P-1 expression vector Escherichia coli
2.7.1.148 gene ispE, recombinant expression of GST-tagged enzyme in Escherichia coli strain DH5alpha from pGEX-6P-1 expression vector Bacillus subtilis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.6.1.12 structures of native IspF and in complex with CMP, to 1.99 A resolution Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.148 Mg2+ required Escherichia coli
2.7.1.148 Mg2+ required Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.148 ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol Escherichia coli
-
 ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?
2.7.1.148 ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol Bacillus subtilis
-
 ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?
2.7.1.148 ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol Bacillus subtilis 168
-
 ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?
2.7.1.148 ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol Bacillus subtilis DSM 23778
-
 ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.148 Bacillus subtilis P37550
-
-
2.7.1.148 Bacillus subtilis 168 P37550
-
-
2.7.1.148 Bacillus subtilis DSM 23778 P37550
-
-
2.7.1.148 Escherichia coli P62615
-
-
4.6.1.12 Bacillus subtilis Q06756
-
-
4.6.1.12 Bacillus subtilis 168 Q06756
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.148 recombinant GST-tagged enzyme from Escherichia coli strain DH5alpha by glutathione affinity chromatography, tag cleavage through Prescission protease, followed by anion exchange chromatography, and gel filtration Bacillus subtilis
2.7.1.148 recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage through thrombin, followed by anion exchange chromatography, and gel filtration Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.148 ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 Escherichia coli ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?
2.7.1.148 ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 Bacillus subtilis ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?
2.7.1.148 ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 Bacillus subtilis 168 ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?
2.7.1.148 ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 Bacillus subtilis DSM 23778 ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?

Subunits

EC Number Subunits Comment Organism
4.6.1.12 More a protein complex assembly of 2-C-methyl-D-erythritol-4-phosphatecytidyltransferase (IspD), CDPME kinase (IspE) and IspF, which catalyze three consecutive steps, is not detectable Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
2.7.1.148 BsIspE
-
 Bacillus subtilis
2.7.1.148 CDPME kinase
-
 Escherichia coli
2.7.1.148 CDPME kinase
-
 Bacillus subtilis
2.7.1.148 EcIspE
-
 Escherichia coli
2.7.1.148 IspE
-
 Escherichia coli
2.7.1.148 IspE
-
 Bacillus subtilis
4.6.1.12 IspF
-
 Bacillus subtilis

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.148 ATP
-
 Escherichia coli
2.7.1.148 ATP
-
 Bacillus subtilis

General Information

EC Number General Information Comment Organism
2.7.1.148 metabolism CDPME kinase (IspE) catalyzes the phosphorylation of CDPME to 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDPME2P) as part of the MEP pathway, overview. In Bacillus subtilis, no stable complexes of BsIspD/BsIspE, BsIspD/BsIspF, and BsIspD/BsIspE/BsIspF are observed, interaction analysis, overview Bacillus subtilis
2.7.1.148 metabolism CDPME kinase (IspE) catalyzes the phosphorylation of CDPME to 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDPME2P) as part of the MEP pathway, overview. In Escherichia coli, no stable complexes of EcIspD/EcIspE, EcIspE/EcIspF, and EcIspD/EcIspE/EcIspF are observed, only the dual mixture of BsIspE and BsIspF seems to merge into a single band, interaction analysis, overview Escherichia coli
2.7.1.148 physiological function CDPME kinase (IspE) catalyzes the phosphorylation of CDPME to 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDPME2P) Escherichia coli
2.7.1.148 physiological function CDPME kinase (IspE) catalyzes the phosphorylation of CDPME to 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDPME2P) Bacillus subtilis