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Literature summary extracted from

  • Kumar, R.P.; Morehouse, B.R.; Matos, J.O.; Malik, K.; Lin, H.; Krauss, I.J.; Oprian, D.D.
    Structural characterization of early Michaelis complexes in the reaction catalyzed by (+)-limonene synthase from Citrus sinensis using fluorinated substrate analogues (2017), Biochemistry, 56, 1716-1725 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.20 expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL cells Citrus sinensis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.3.20 in complex with 2-fluorogeranyl diphosphate or 2-fluoroneryl diphosphate, vapor diffusion method Citrus sinensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.3.20 2-Fluorogeranyl diphosphate competitive inhibitor Citrus sinensis
4.2.3.20 2-fluoroneryl diphosphate competitive inhibitor Citrus sinensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.20 geranyl diphosphate Citrus sinensis
-
 (R)-limonene + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.20 Citrus sinensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.20 Ni2+-affinity column chromatography Citrus sinensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.20 2-fluorogeranyl diphosphate
-
 Citrus sinensis ?
-
 ?
4.2.3.20 2-fluoroneryl diphosphate
-
 Citrus sinensis ?
-
 ?
4.2.3.20 geranyl diphosphate
-
 Citrus sinensis (R)-limonene + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.20 (+)-limonene synthase
-
 Citrus sinensis
4.2.3.20 (+)-LS
-
 Citrus sinensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.3.20 0.00024
-
 2-fluoroneryl diphosphate pH and temperature not specified in the publication Citrus sinensis
4.2.3.20 0.00054
-
 2-Fluorogeranyl diphosphate pH and temperature not specified in the publication Citrus sinensis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.3.20 0.0024
-
 2-Fluorogeranyl diphosphate pH and temperature not specified in the publication Citrus sinensis
4.2.3.20 0.0395
-
 2-fluoroneryl diphosphate pH and temperature not specified in the publication Citrus sinensis