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Literature summary extracted from
- Expanding the threonine aldolase toolbox for the asymmetric synthesis of tertiary alpha-amino acids (2015), Appl. Microbiol. Biotechnol., 99, 9651-9661 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.1.2.5 | overexpression in Escherichia coli | Aeromonas veronii |
| 4.1.2.42 | overexpression in Escherichia coli | Pseudomonas sp. |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.2.5 | 2 | - |
D-beta-phenylserine | 25°C, pH 8.0 | Aeromonas veronii |  |
| 4.1.2.5 | 2.76 | - |
alpha-methyl-beta-phenylserine | 25°C, pH 8.0 | Aeromonas veronii | |
| 4.1.2.5 | 10.9 | - |
D-threonine | pH 8.0, temperature not specified in the publication | Aeromonas veronii | |
| 4.1.2.42 | 1.5 | - |
D-beta-phenylserine | 25°C, pH 8.0 | Pseudomonas sp. | |
| 4.1.2.42 | 6.2 | - |
alpha-methyl-beta-phenylserine | 25°C, pH 8.0 | Pseudomonas sp. | |
| 4.1.2.42 | 15.9 | - |
D-threonine | pH 8.0, temperature not specified in the publication | Pseudomonas sp. | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.2.5 | Aeromonas veronii | - |
- |
- |
| 4.1.2.42 | Pseudomonas sp. | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.1.2.5 | - |
Aeromonas veronii |
| 4.1.2.42 | - |
Pseudomonas sp. |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.2.5 | alpha-methyl-beta-phenylserine | - |
Aeromonas veronii | ? | - |
? | |
| 4.1.2.5 | D-alanine + 3-nitrobenzaldehyde | 23% conversion | Aeromonas veronii | beta-(3-nitrophenyl)-alpha-methyl-serine | - |
? | |
| 4.1.2.5 | D-alanine + benzaldehyde | 10% conversion | Aeromonas veronii | 2-amino-3-hydroxy-2-methyl-3-phenylpropanoic acid | - |
? | |
| 4.1.2.5 | D-alanine + hexanal | 4% conversion | Aeromonas veronii | 2-amino-3-hydroxy-2-methyloctanoic acid | - |
? | |
| 4.1.2.5 | D-beta-phenylserine | - |
Aeromonas veronii | glycine + benzaldehyde | - |
? | |
| 4.1.2.5 | D-serine + 3-nitrobenzaldehyde | 2% conversion | Aeromonas veronii | beta-(3-nitrophenyl)-alpha-hydroxymethyl-serine | - |
? | |
| 4.1.2.5 | D-serine + benzaldehyde | 4% conversion | Aeromonas veronii | 2-amino-3-hydroxy-2-hydroxymethyl-3-phenylpropanoic acid | - |
? | |
| 4.1.2.5 | D-threonine | - |
Aeromonas veronii | glycine + acetaldehyde | - |
? | |
| 4.1.2.42 | alpha-methyl-beta-phenylserine | - |
Pseudomonas sp. | D-alanine + benzaldehyde | - |
? | |
| 4.1.2.42 | D-alanine + 3-nitrobenzaldehyde | - |
Pseudomonas sp. | beta-(3-nitrophenyl)-alpha-methyl-serine | - |
? | |
| 4.1.2.42 | D-alanine + benzaldehyde | - |
Pseudomonas sp. | 2-amino-3-hydroxy-2-methyl-3-phenylpropanoic acid | - |
? | |
| 4.1.2.42 | D-alanine + hexanal | - |
Pseudomonas sp. | 2-amino-3-hydroxy-2-methyloctanoate | - |
? | |
| 4.1.2.42 | D-beta-phenylserine | - |
Pseudomonas sp. | glycine + benzaldehyde | - |
? | |
| 4.1.2.42 | D-serine + 3-nitrobenzaldehyde | - |
Pseudomonas sp. | beta-(3-nitrophenyl)-alpha-hydroxymethyl-serine | - |
? | |
| 4.1.2.42 | D-serine + benzaldehyde | - |
Pseudomonas sp. | 2-amino-3-hydroxy-2-hydroxymethyl-3-phenylpropanoic acid | - |
? | |
| 4.1.2.42 | D-threonine | - |
Pseudomonas sp. | glycine + acetaldehyde | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.1.2.5 | LTA | - |
Aeromonas veronii |
| 4.1.2.42 | DTA | - |
Pseudomonas sp. |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.2.5 | 0.2 | - |
D-threonine | pH 8.0, temperature not specified in the publication | Aeromonas veronii | |
| 4.1.2.5 | 0.54 | - |
alpha-methyl-beta-phenylserine | 25°C, pH 8.0 | Aeromonas veronii | |
| 4.1.2.5 | 18.3 | - |
D-beta-phenylserine | 25°C, pH 8.0 | Aeromonas veronii | |
| 4.1.2.42 | 0.033 | - |
alpha-methyl-beta-phenylserine | 25°C, pH 8.0 | Pseudomonas sp. | |
| 4.1.2.42 | 0.4 | - |
D-threonine | pH 8.0, temperature not specified in the publication | Pseudomonas sp. | |
| 4.1.2.42 | 1.4 | - |
D-beta-phenylserine | 25°C, pH 8.0 | Pseudomonas sp. | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.2.5 | pyridoxal 5'-phosphate | pyridoxal 5'-phosphate-dependent enzyme | Aeromonas veronii | |
| 4.1.2.42 | pyridoxal 5'-phosphate | pyridoxal 5'-phosphate-dependent enzyme | Pseudomonas sp. | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.2.5 | 0.0027 | - |
D-threonine | pH 8.0, temperature not specified in the publication | Aeromonas veronii | |
| 4.1.2.5 | 0.195 | - |
alpha-methyl-beta-phenylserine | 25°C, pH 8.0 | Aeromonas veronii | |
| 4.1.2.5 | 9.2 | - |
D-beta-phenylserine | 25°C, pH 8.0 | Aeromonas veronii | |
| 4.1.2.42 | 0.00533 | - |
alpha-methyl-beta-phenylserine | 25°C, pH 8.0 | Pseudomonas sp. | |
| 4.1.2.42 | 0.025 | - |
D-threonine | pH 8.0, temperature not specified in the publication | Pseudomonas sp. | |
| 4.1.2.42 | 0.91 | - |
D-beta-phenylserine | 25°C, pH 8.0 | Pseudomonas sp. | |
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