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Literature summary extracted from

  • Zafar, A.; Aftab, M.; ud Din, Z.; Aftab, S.; Iqbal, I.; ul Haq, I.
    Cloning, purification and characterization of a highly Thermostable Amylase Gene of Thermotoga petrophila into Escherichia coli (2016), Appl. Biochem. Biotechnol., 178, 831-848 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.2 industry the recombinant purified alpha-amylase enzyme from Thermotoga petrophila can be a potential candidate for use in many industrial processes; particularly, it can be helpful in starch saccharification due to the adaptive capability of the alpha-amylase to withstand unreceptive conditions used in industrial processes Thermotoga petrophila

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.2 expression in Escherichia coli BL21 (DE3), pET 21a (+) vector Thermotoga petrophila

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.2 2-mercaptoethanol 4%, 1h, activity is decreased to 53% Thermotoga petrophila
3.2.1.2 acetone 40%, relative activity is decreased to 87% Thermotoga petrophila
3.2.1.2 Co2+ 1 mM, 37% loss of activity Thermotoga petrophila
3.2.1.2 EDTA 1 mM, 42% loss of activity. 5-10 mM, complete loss of activity Thermotoga petrophila
3.2.1.2 Isopropanol 40%, relative activity is decreased to 80% Thermotoga petrophila
3.2.1.2 K+ 1 mM, 1% loss of activity Thermotoga petrophila
3.2.1.2 Mg2+ 1 mM, 14% loss of activity Thermotoga petrophila
3.2.1.2 additional information no considerable effect of organic solvents (ethanol, methanol, isopropanol, acetone and n-butanol) is observed on enzyme activity Thermotoga petrophila
3.2.1.2 n-butanol 40%, relative activity is decreased to 85% Thermotoga petrophila
3.2.1.2 Ni2+ 1 mM, 28% loss of activity Thermotoga petrophila
3.2.1.2 SDS 4%, 1 h, complete loss of activity Thermotoga petrophila
3.2.1.2 Tween 20 4%, 1h, activity is decreased to 71% Thermotoga petrophila
3.2.1.2 Tween 80 4%, 1h, activity is decreased to 61% Thermotoga petrophila

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.2 Ca2+ activity is increased to 150 % in the presence of calcium chloride Thermotoga petrophila

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.2 70000
-
 SDS-PAGE Thermotoga petrophila

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.2 Thermotoga petrophila A0A0U4KTC9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.2
-
 Thermotoga petrophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.2 amylopektin + H2O
-
 Thermotoga petrophila ?
-
 ?
3.2.1.2 dextrin + H2O
-
 Thermotoga petrophila ?
-
 ?
3.2.1.2 soluble starch + H2O maximum activity with 1.2 % soluble starch. Less activity is observed with starch from corn, rice, glycogen Thermotoga petrophila maltose + ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.2 85
-
-
 Thermotoga petrophila

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.2 50
-
 2 h, purified enzyme retains 100 % relative activity. Ater 4 h of incubation, the relative activity of the enzyme is 80% Thermotoga petrophila
3.2.1.2 60
-
 2 h, purified enzyme retains 100 % relative activity. Ater 4 h of incubation, the relative activity of the enzyme is 80% Thermotoga petrophila
3.2.1.2 70
-
 2 h, purified enzyme retains 100 % relative activity. Ater 4 h of incubation, the relative activity of the enzyme is 72% Thermotoga petrophila
3.2.1.2 80
-
 2 h, purified enzyme retains 100 % relative activity. Ater 4 h of incubation, the relative activity of the enzyme is 63% Thermotoga petrophila
3.2.1.2 90
-
 2 h, 15% loss of activity. Ater 4 h of incubation, the relative activity of the enzyme is 54% Thermotoga petrophila
3.2.1.2 100
-
 2 h, 22% loss of activity. Ater 4 h of incubation, the relative activity of the enzyme is 45% Thermotoga petrophila

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.2 4
-
 after 1 h of incubation, the relative activity of the enzyme is 20% Thermotoga petrophila
3.2.1.2 5
-
 after 1 h of incubation, the relative activity of the enzyme is 30% Thermotoga petrophila
3.2.1.2 6
-
 the enzyme exhibits 80% relative activity after 4 h Thermotoga petrophila
3.2.1.2 7
-
 maximum relative activity (100%) is obtained after 4 h at pH 7.0 Thermotoga petrophila
3.2.1.2 8
-
 the enzyme exhibits 70% relative activity after 3 h Thermotoga petrophila
3.2.1.2 9
-
 activity is decreased to 30% after 1 h of incubation Thermotoga petrophila