Any feedback?
Literature summary extracted from
- Recombinant S-adenosylhomocysteine hydrolase from thermotoga maritima Cloning, overexpression, characterization, and thermal purification studies (2013), Appl. Biochem. Biotechnol., 170, 639-653 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.13.2.1 | overexpressed using Escherichia coli | Thermotoga maritima |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.13.2.1 | 0.018 | - |
S-adenosyl-L-homocysteine | pH 6.5, 75°C | Thermotoga maritima |  |
| 3.13.2.1 | 0.049 | - |
adenosine | pH 6.5, 75°C | Thermotoga maritima | |
| 3.13.2.1 | 0.135 | - |
L-homocysteine | pH 6.5, 75°C | Thermotoga maritima | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.13.2.1 | 180000 | - |
gel filtration | Thermotoga maritima |
Organic Solvent Stability
| EC Number | Organic Solvent | Comment | Organism |
|---|---|---|---|
| 3.13.2.1 | urea | the enzyme is stable in the presence of urea 6 M when pre-incubated at room temperature for 48 h | Thermotoga maritima |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.13.2.1 | Thermotoga maritima | O51933 | - |
- |
| 3.13.2.1 | Thermotoga maritima DSM 3109 | O51933 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.13.2.1 | partially | Thermotoga maritima |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.13.2.1 | -20°C, pH 6.5, 50 mM phosphate buffer, stable for at least 3 months | Thermotoga maritima |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.13.2.1 | S-adenosyl-L-homocysteine + H2O | - |
Thermotoga maritima | L-homocysteine + adenosine | - |
? | |
| 3.13.2.1 | S-adenosyl-L-homocysteine + H2O | - |
Thermotoga maritima DSM 3109 | L-homocysteine + adenosine | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.13.2.1 | tetramer | 4 * 44000, SDS-PAGE | Thermotoga maritima |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.13.2.1 | S-adenosylhomocysteine hydrolase | - |
Thermotoga maritima |
| 3.13.2.1 | SAHase | - |
Thermotoga maritima |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.13.2.1 | 75 | - |
- |
Thermotoga maritima |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.13.2.1 | 60 | 85 | 60°C: about 65% of maximal activity, 85°C: about 75% of maximal activity | Thermotoga maritima |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.13.2.1 | 75 | - |
120 min, 5% loss of activity | Thermotoga maritima |
| 3.13.2.1 | 87.6 | - |
melting temperature | Thermotoga maritima |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.13.2.1 | 6.5 | - |
- |
Thermotoga maritima |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.13.2.1 | 6 | 7 | pH 6.0: about 65% of maximal activity, pH 7.0: about 55% of maximal activity | Thermotoga maritima |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
