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Literature summary extracted from

  • Eichhorn, E.; Locher, E.; Guillemer, S.; Wahler, D.; Fourage, L.; Schilling, B.
    Biocatalytic process for (-)-ambrox production using squalene hopene cyclase (2018), Adv. Synth. Catal., 360, 2339-2351 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.99.17 expressed in Escherichia coli BL21(DE3) cells Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446

Protein Variants

EC Number Protein Variants Comment Organism
5.4.99.17 F129L the mutant shows a (E,E)-homofarnesol conversion at 24 h of reaction which is comparable to the wild type enzyme Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446
5.4.99.17 F129L/F601Y the mutant shows lower (E,E)-homofarnesol conversion at 24 h of reaction compared to the wild type enzyme Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446
5.4.99.17 F129L/M132R/I432T the mutant shows much lower (E,E)-homofarnesol conversion at 24 h of reaction compared to the wild type enzyme Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446
5.4.99.17 F129L/M132R/I432T/F601Y the mutant shows much lower (E,E)-homofarnesol conversion compared to the wild type enzyme Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446
5.4.99.17 F601Y the mutant shows a (E,E)-homofarnesol conversion at 24 h of reaction which is comparable to the wild type enzyme Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446
5.4.99.17 M132R/A224V/I432T the mutant shows better (E,E)-homofarnesol conversion at 24 h of reaction compared to the wild type enzyme Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446
5.4.99.17 M132R/I432T the mutant shows better (E,E)-homofarnesol conversion at 24 h of reaction compared to the wild type enzyme Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446
5.4.99.17 M132R/I432T/F601Y the mutant shows much lower (E,E)-homofarnesol conversion at 24 h of reaction compared to the wild type enzyme Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446
5.4.99.17 Q579H/F601Y the mutant shows better (E,E)-homofarnesol conversion at 24 h of reaction compared to the wild type enzyme Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446
5.4.99.17 T77A/I92V/F129L the mutant shows better (E,E)-homofarnesol conversion at 24 h of reaction compared to the wild type enzyme Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.99.17 squalene Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446
-
 hop-22(29)-ene
-
 ?
5.4.99.17 squalene + H2O Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446
-
 hopan-22-ol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.17 Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446 P33247
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.17 (E,E)-homofarnesol
-
 Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446 (-)-ambrox + (3aS,5aS,9aS,9bS)-3a,6,6,9a-tetramethyldodeca-hydronaphtho[2,1-b]furan
-
 ?
5.4.99.17 (E,Z)-homofarnesol
-
 Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446 (4Z,7aS,11aS)-5,8,8,11a-tetramethyl-2,3,6,7,7a,8,9,10,11,11a-decahydrobenzo[b]oxonine + 9-epi-ambrox
-
 ?
5.4.99.17 squalene
-
 Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446 hop-22(29)-ene
-
 ?
5.4.99.17 squalene + H2O
-
 Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446 hopan-22-ol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.4.99.17 SHC
-
 Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446
5.4.99.17 squalene hopene cyclase
-
 Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.4.99.17 55
-
-
 Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4.99.17 6
-
-
 Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446