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Literature summary extracted from

  • Zheng, Y.; Xu, J.; Wang, H.; Lin, G.; Hong, R.; Yu, H.
    Hydroxynitrile lyase isozymes from Prunus communis identification, characterization and synthetic applications (2017), Adv. Synth. Catal., 359, 1185-1193 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.10 heterologously expression of the His-tagged hydroxynitrile lyase isoenzymes (HNL1, HNL2, HNL4 and HNL5) in Pichia pastoris Prunus dulcis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.10 1.09
-
 (R)-4-methylsulfanylbenzaldehyde pH 4.2, 25°C, isoenzyme HNL2 Prunus dulcis
4.1.2.10 1.16
-
 (R)-mandelonitrile pH 4.2, 25°C, isoenzyme HNL1 Prunus dulcis
4.1.2.10 1.47
-
 (R)-mandelonitrile pH 4.2, 25°C, isoenzyme HNL2 Prunus dulcis
4.1.2.10 1.54
-
 (R)-4-methylsulfanylbenzaldehyde pH 4.2, 25°C, isoenzyme HNL4 Prunus dulcis
4.1.2.10 1.91
-
 (R)-mandelonitrile pH 4.2, 25°C, isoenzyme HNL4 Prunus dulcis
4.1.2.10 2.29
-
 (R)-4-methylsulfanylbenzaldehyde pH 4.2, 25°C, isoenzyme HNL1 Prunus dulcis
4.1.2.10 2.97
-
 (R)-4-methylsulfanylbenzaldehyde pH 4.2, 25°C, isoenzyme HNL5 Prunus dulcis
4.1.2.10 6.36
-
 (R)-mandelonitrile pH 4.2, 25°C, isoenzyme HNL5 Prunus dulcis

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.10 Prunus dulcis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.10 hydroxynitrile lyase isoenzymes (HNL1, HNL2, HNL4 and HNL5) Prunus dulcis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.10 (R)-mandelonitrile
-
 Prunus dulcis cyanide + benzaldehyde
-
 r
4.1.2.10 cyanide + (R)-4-methylsulfanylbenzaldehyde
-
 Prunus dulcis (R)-4-methylsulfanyl-mandelonitrile
-
 ?
4.1.2.10 cyanide + 2,3,4a,8a-tetrahydro-1,4-benzodioxine-6-carbaldehyde isoenzyme HNL5 Prunus dulcis (2R)-hydroxy(2,3,4a,8a-tetrahydro-1,4-benzodioxin-6-yl)acetonitrile
-
 ?
4.1.2.10 cyanide + 2-chlorobenzaldehyde
-
 Prunus dulcis (R)-2-chloromandelonitrile
-
 r
4.1.2.10 cyanide + 2-methoxybenzaldehyde
-
 Prunus dulcis (R)-2-methoxymandelonitrile
-
 r
4.1.2.10 cyanide + 3-chlorobenzaldehyde
-
 Prunus dulcis (R)-3-chloromandelonitrile
-
 r
4.1.2.10 cyanide + 3-methoxybenzaldehyde
-
 Prunus dulcis (R)-3-methoxymandelonitrile
-
 r
4.1.2.10 cyanide + 3-phenoxybenzaldehyde
-
 Prunus dulcis (R)-3-phenoxymandelonitrile
-
 r
4.1.2.10 cyanide + 3-phenylpropanal isoenzyme HNL5 Prunus dulcis (R)-2-hydroxy-4-phenylbutyronitrile
-
 ?
4.1.2.10 cyanide + 4-fluorobenzaldehyde
-
 Prunus dulcis (R)-4-fluoromandelonitrile
-
 r
4.1.2.10 cyanide + 4-methoxybenzaldehyde
-
 Prunus dulcis (R)-4-methoxymandelonitrile
-
 r
4.1.2.10 cyanide + 4-methylbenzaldehyde
-
 Prunus dulcis (R)-4-methylmandelonitrile
-
 r
4.1.2.10 cyanide + benzaldehyde
-
 Prunus dulcis (R)-mandelonitrile
-
 r
4.1.2.10 cyanide + furan-2-carbaldehyde isoenzyme HNL5 Prunus dulcis (2R)-(furan-2-yl)(hydroxy)acetonitrile
-
 ?
4.1.2.10 cyanide + naphthalene-1-carbaldehyde isoenzyme HNL5 Prunus dulcis (2R)-hydroxy(naphthalen-1-yl)acetonitrile
-
 ?
4.1.2.10 cyanide + naphthalene-2-carbaldehyde isoenzyme HNL5 Prunus dulcis (2R)-hydroxy(naphthalen-2-yl)acetonitrile
-
 ?
4.1.2.10 cyanide + phenylacetaldehyde isoenzyme HNL5 Prunus dulcis (2R)-2-hydroxy-3-phenylpropanenitrile
-
 ?
4.1.2.10 cyanide + pyridine-3-carbaldehyde isoenzyme HNL5 Prunus dulcis (2R)-hydroxy(pyridin-3-yl)acetonitrile
-
 ?
4.1.2.10 cyanide + thiophene-2-carbaldehyde isoenzyme HNL5 Prunus dulcis (2R)-hydroxy(thiophen-2-yl)acetonitrile
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.2.10 HNL1
-
 Prunus dulcis
4.1.2.10 HNL2
-
 Prunus dulcis
4.1.2.10 HNL4
-
 Prunus dulcis
4.1.2.10 HNL5
-
 Prunus dulcis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.2.10 26.3
-
 (R)-4-methylsulfanylbenzaldehyde pH 4.2, 25°C, isoenzyme HNL4 Prunus dulcis
4.1.2.10 40.3
-
 (R)-4-methylsulfanylbenzaldehyde pH 4.2, 25°C, isoenzyme HNL2 Prunus dulcis
4.1.2.10 139
-
 (R)-4-methylsulfanylbenzaldehyde pH 4.2, 25°C, isoenzyme HNL1 Prunus dulcis
4.1.2.10 142
-
 (R)-4-methylsulfanylbenzaldehyde pH 4.2, 25°C, isoenzyme HNL5 Prunus dulcis
4.1.2.10 240
-
 (R)-mandelonitrile pH 4.2, 25°C, isoenzyme HNL1 Prunus dulcis
4.1.2.10 380
-
 (R)-mandelonitrile pH 4.2, 25°C, isoenzyme HNL2 Prunus dulcis
4.1.2.10 817
-
 (R)-mandelonitrile pH 4.2, 25°C, isoenzyme HNL5 Prunus dulcis
4.1.2.10 818
-
 (R)-mandelonitrile pH 4.2, 25°C, isoenzyme HNL4 Prunus dulcis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.1.2.10 17
-
 (R)-4-methylsulfanylbenzaldehyde pH 4.2, 25°C, isoenzyme HNL4 Prunus dulcis
4.1.2.10 37
-
 (R)-4-methylsulfanylbenzaldehyde pH 4.2, 25°C, isoenzyme HNL2 Prunus dulcis
4.1.2.10 48
-
 (R)-4-methylsulfanylbenzaldehyde pH 4.2, 25°C, isoenzyme HNL5 Prunus dulcis
4.1.2.10 61
-
 (R)-4-methylsulfanylbenzaldehyde pH 4.2, 25°C, isoenzyme HNL1 Prunus dulcis
4.1.2.10 128
-
 (R)-mandelonitrile pH 4.2, 25°C, isoenzyme HNL5 Prunus dulcis
4.1.2.10 207
-
 (R)-mandelonitrile pH 4.2, 25°C, isoenzyme HNL1 Prunus dulcis
4.1.2.10 258
-
 (R)-mandelonitrile pH 4.2, 25°C, isoenzyme HNL2 Prunus dulcis
4.1.2.10 428
-
 (R)-mandelonitrile pH 4.2, 25°C, isoenzyme HNL4 Prunus dulcis