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Literature summary extracted from

  • Do, H.; Lee, C.W.; Han, S.J.; Lee, S.G.; Kim, H.J.; Park, H.; Lee, J.H.
    Purification, crystallization and preliminary X-ray crystallographic studies of FMN-bound and FMN-free forms of aromatic acid decarboxylase (CpsUbiX) from the psychrophilic bacterium Colwellia psychrerythraea 34H (2014), Acta Crystallogr. Sect. F, 70, 215-220 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.98 gene ubiX, recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Colwellia psychrerythraea

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.1.98 purified recombinant His6-tagged wild-type (FMN-bound) and V47S mutant (FMN-free) enzymes, hanging drop vapour diffusion method, mixing of 800 nl of 21 mg/ml protein solution with 800 nl of reservoir solution containing 200 mM sodium chloride, 100 mM potassium phosphate monobasic/sodium phosphate dibasic, pH 5.8, and 11% w/v PEG 8000, for the wild-type enzyme and 0.1 M trisodium citrate, pH 5.4, 0.5 M ammonium sulfate, and 1.2 M lithium sulfate for the mutant enzyme, 20°C, 3 days, method optimization, X-ray diffraction structrue determination and analysis at 2.0 A and 1.76 A resolution, respectively Colwellia psychrerythraea

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.98 V47S site-directed mutagenesis, the single mutation results in a loss of FMN binding, resulting in the production of FMN-free CpsUbiX protein. The mutation is likely to destabilize FMN-protein interactions without affecting the overall structural folding. The mutant enzyme has an FMN:protein molar ratio of 0.086:1. Wild-type CpsUbiX protein has a yellowish colour, while the V47S mutant does not Colwellia psychrerythraea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.98 256300
-
 recombinant wild-type enzyme Colwellia psychrerythraea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.98 a 4-hydroxy-3-polyprenylbenzoate Colwellia psychrerythraea
-
 a 2-polyprenylphenol + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.98 Colwellia psychrerythraea Q489U8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.98 recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and ultrafiltration Colwellia psychrerythraea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.98 a 4-hydroxy-3-polyprenylbenzoate
-
 Colwellia psychrerythraea a 2-polyprenylphenol + CO2
-
 ?
4.1.1.98 additional information analysis of the conformational changes upon FMN binding and the enzymatic mechanism at the molecular level, overview Colwellia psychrerythraea ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.98 dodecamer 12 * 22552, sequence calculation Colwellia psychrerythraea

Synonyms

EC Number Synonyms Comment Organism
4.1.1.98 CpsUbiX
-
 Colwellia psychrerythraea
4.1.1.98 flavin mononucleotide-dependent aromatic acid decarboxylase
-
 Colwellia psychrerythraea
4.1.1.98 FMN-dependent aromatic acid decarboxylase
-
 Colwellia psychrerythraea
4.1.1.98 ubiX
-
 Colwellia psychrerythraea

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.98 FMN wild-type CpsUbiX has an FMN:protein molar ratio of 1.028:1. The protein with a V47S single mutation cannot bind FMN and has an FMN:protein molar ratio of 0.086:1 Colwellia psychrerythraea

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.1.1.98 Colwellia psychrerythraea sequence calculation
-
 5.78

General Information

EC Number General Information Comment Organism
4.1.1.98 evolution UbiX belongs to the FMN-dependent decarboxylase family Colwellia psychrerythraea
4.1.1.98 metabolism a set of enzymes, UbiA, UbiB, UbiC, UbiD, UbiE, UbiF, UbiG, UbiH and UbiX, catalyze the synthesis of ubiquinone from the compound chorismate. Enzyme UbiX catalyzes and regulates ubiquinone biosynthesis Colwellia psychrerythraea