Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Cui, L.; Ni, X.; Ji, Q.; Teng, X.; Yang, Y.; Wu, C.; Zekria, D.; Zhang, D.; Kai, G.
    Co-overexpression of geraniol-10-hydroxylase and strictosidine synthase improves anti-cancer drug camptothecin accumulation in Ophiorrhiza pumila (2015), Sci. Rep., 5, 8227 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.14.14.83 drug development co-overexpression of geraniol-10-hydroxylase and strictosidine synthase improves anti-cancer drug camptothecin accumulation in Ophiorrhiza pumila Catharanthus roseus
1.14.14.83 pharmacology co-overexpression of geraniol-10-hydroxylase and strictosidine synthase improves anti-cancer drug camptothecin accumulation in Ophiorrhiza pumila Catharanthus roseus
1.14.14.83 synthesis introduction of genes encoding strictosidine synthase (STR) and geraniol 8-hydroxylase (G10H), into Ophiorrhiza pumila hairy roots. Overexpression of individual G10H significantly improves camptothecin prdouction. Cooverexpression of G10H and STR genes causes a 56% increase on the yields of camptothecin. Camptothecin extracted from different lines shows similar anti-tumor activity Catharanthus roseus
4.3.3.2 drug development cooverexpression of geraniol-10-hydroxylase and strictosidine synthase improves anti-cancer drug camptothecin accumulation in Ophiorrhiza pumila Catharanthus roseus
4.3.3.2 pharmacology cooverexpression of geraniol-10-hydroxylase and strictosidine synthase improves anti-cancer drug camptothecin accumulation in Ophiorrhiza pumila Catharanthus roseus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.83 co-overexpression of geraniol-10-hydroxylase and strictosidine synthase in Ophiorrhiza pumila Catharanthus roseus
1.14.14.83 gene CrG10H, recombinant expression in transgenic Ophiorrhiza pumila hairy roots using Agrobacterium rhizogenes strain C58C1 for transfection Catharanthus roseus
4.3.3.2 cooverexpression of geraniol-10-hydroxylase and strictosidine synthase in Ophiorrhiza pumila Catharanthus roseus

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.83 additional information overexpression of individual geraniol 10-hydroxylase, G10H (G lines), significantly improves camptothecin (CPT) production with respect to non-transgenic hairy root cultures (NC line) and single strictosidine synthase (STR, EC 4.3.3.2) overexpressing lines (S lines), indicating that G10H plays a more important role in stimulating CPT accumulation than STR in Ophiorrhiza pumila. Co-overexpression of G10H and STR genes (SG Lines) causes a 56% increase on the yields of CPT compared to NC line and single gene transgenic lines, show that simultaneous introduction of G10H and STR can produce a synergistic effect on CPT biosynthesis in Ophiorrhiza pumila. camptothecin accumulates in the hairy roots. G10H is a key regulation target gene for metabolic engineering of CPT biosynthetic pathway Catharanthus roseus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.83 geraniol + [reduced NADPH-hemoprotein reductase] + O2 Catharanthus roseus
-
 10-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.83 Catharanthus roseus Q8VWZ7
-
-
4.3.3.2 Catharanthus roseus P18417
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.83 geraniol + [reduced NADPH-hemoprotein reductase] + O2
-
 Catharanthus roseus 10-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.83 CYP76B6 UniProt Catharanthus roseus
1.14.14.83 G10H
-
 Catharanthus roseus
1.14.14.83 geraniol 10-hydroxylase
-
 Catharanthus roseus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.83 NADPH
-
 Catharanthus roseus

General Information

EC Number General Information Comment Organism
1.14.14.83 metabolism the enzyme catalyzes the first committed step in the camptothecin biosynthetic pathway and a key regulation point of the campothecin biosynthetic pathway Catharanthus roseus
1.14.14.83 physiological function the enzyme plays an important role in stimulating campothecin accumulation, capothecin has anti-tumor activity Catharanthus roseus