Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • de Queiroz Brito Cunha, C.C.; Gama, A.R.; Cintra, L.C.; Bataus, L.A.M.; Ulhoa, C.J.
    Improvement of bread making quality by supplementation with a recombinant xylanase produced by Pichia pastoris (2018), PLoS ONE, 13, e0192996 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.8 2-mercaptoethanol 5 mM, 152% of initial activity Streptomyces sp.

Application

EC Number Application Comment Organism
3.2.1.8 food industry use of enzyme as an additive in the bread making process leads to a decrease in firmness, stiffness and consistency, and improvements in specific volume and reducing sugar content Streptomyces sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.8 expression in Pichia pastoris Streptomyces sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.8 Ag+ 5 mM, no residual activity Streptomyces sp.
3.2.1.8 Cu2+ 5 mM, 72% residual activity Streptomyces sp.
3.2.1.8 Hg2+ 5 mM, 34% residual activity Streptomyces sp.
3.2.1.8 Mn2+ 5 mM, 54% residual activity Streptomyces sp.
3.2.1.8 SDS 5 mM, 51% residual activity Streptomyces sp.
3.2.1.8 xylose 40 mM, 81% residual activity Streptomyces sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.8 additional information
-
 beechwood xylan Km value 12.4 mg/ml, pH 6.0, 50°C Streptomyces sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.8 Ba2+ 5 mM, 142% of initial activity Streptomyces sp.
3.2.1.8 Mg2+ 5 mM, 135% of initial activity Streptomyces sp.
3.2.1.8 NH4Cl 5 mM, 142% of initial activity Streptomyces sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Streptomyces sp. D1FNQ6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8 recombinant protein Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 beechwood xylan + H2O
-
 Streptomyces sp. ?
-
 ?
3.2.1.8 oat spelt xylan + H2O
-
 Streptomyces sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 ? x * 20000, SDS-PAGE Streptomyces sp.

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 XynBS27
-
 Streptomyces sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 75
-
-
 Streptomyces sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 6
-
-
 Streptomyces sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.8 4.5 8.5 more than 60% of maximum activity Streptomyces sp.