EC Number | Cloned (Comment) | Organism |
---|---|---|
1.13.12.2 | gene davB, recombinant expression in Escherichia coli, coexpression with gene davA, encoding 5-aminovaleramidase, recombinant expression of gene davB as N-terminally His-tagged enzyme in Corynebacterium glutamicum, a highly L-lysine producing strain BE using the sod promoter and the transcription factor Tu, EC 3.6.5.3 | Pseudomonas putida |
3.5.1.30 | gene davA, recombinant expression in Corynebacterium glutamicum, coexpression with gene davB from Pseudomonas putida encoding lysine 2-monooxygenase (EC 1.13.12.2) | Pseudomonas putida |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.13.12.2 | additional information | engineering of a recombinant Escherichia coli strain expressing the davB and davA genes for bioconversion of L-lysine to 5-aminovaleric acid resulting in low levels of 5-aminovalerate. Development of metabolically engineered Corynebacterium glutamicum strains for enhanced fermentative production of 5-aminovalerate from glucose. Expression of the Corynebacterium glutamicum codon-optimized davA gene fused with His6-Tag at its N-terminus and the davB gene as an operon under a strong synthetic H36 promoter (plasmid p36davAB3) in Corynebacterium glutamicum strain BE (pJS38) enables the most efficient production of 5-aminovalerate. The construct containing the His6-tagged variant produces substantially more 5-aminovalerate compared to that produced using the construct lacking the His-tag, possibly because of the improved stability afforded by the 5'-modification, which results in higher expression of the davAB genes in the recombinant Corynebacterium glutamicum BE strain. Deletion of the gabT gene (EC 2.6.1.19), encoding 4-aminobutyrate aminotransferase, improves the 5-aminovalerate production | Pseudomonas putida |
3.5.1.30 | additional information | combined expression of Pseudomonas putida davB and davA genes encoding lysine 2-monooxygenase and delta-aminovaleramidase, respectively, in engineered Corynebacterium glutamicum strain BE (KCTC 12390BP) for large scale production of 5-aminovalerate (5AVA) under the control of constitutive promoters. Expression of codon-optimized N-terminally His6-tagged davA gene fused the davB gene as an operon under a strong synthetic H36 promoter from plasmid p36davAB3 in Corynebacterium glutamicum in fed-batch culture enables the most efficient production of 5AVA. In addition, production of glutaric acid, a major byproduct, is significantly reduced by employing Corynebacterium glutamicum gabT mutant as a host. Method development and optimization, overview | Pseudomonas putida |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.12.2 | L-lysine + O2 | Pseudomonas putida | - |
5-aminopentanamide + CO2 + H2O | - |
? | |
1.13.12.2 | L-lysine + O2 | Pseudomonas putida ATCC 12633 | - |
5-aminopentanamide + CO2 + H2O | - |
? | |
3.5.1.30 | 5-aminopentanamide + H2O | Pseudomonas putida | - |
5-aminopentanoate + NH3 | - |
? | |
3.5.1.30 | 5-aminopentanamide + H2O | Pseudomonas putida ATCC 12633 | - |
5-aminopentanoate + NH3 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.12.2 | Pseudomonas putida | - |
- |
- |
1.13.12.2 | Pseudomonas putida ATCC 12633 | - |
- |
- |
3.5.1.30 | Pseudomonas putida | B3IVI7 | - |
- |
3.5.1.30 | Pseudomonas putida ATCC 12633 | B3IVI7 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.12.2 | L-lysine + O2 | - |
Pseudomonas putida | 5-aminopentanamide + CO2 + H2O | - |
? | |
1.13.12.2 | L-lysine + O2 | - |
Pseudomonas putida ATCC 12633 | 5-aminopentanamide + CO2 + H2O | - |
? | |
3.5.1.30 | 5-aminopentanamide + H2O | - |
Pseudomonas putida | 5-aminopentanoate + NH3 | - |
? | |
3.5.1.30 | 5-aminopentanamide + H2O | - |
Pseudomonas putida ATCC 12633 | 5-aminopentanoate + NH3 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.13.12.2 | davB | - |
Pseudomonas putida |
1.13.12.2 | L-lysine 2-monooxygenase | - |
Pseudomonas putida |
3.5.1.30 | 5-aminovaleramidase | - |
Pseudomonas putida |
3.5.1.30 | DavA | - |
Pseudomonas putida |
3.5.1.30 | delta-aminovaleramidase | - |
Pseudomonas putida |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.13.12.2 | metabolism | the enzyme is involved in the aminovalerate pathway, overview | Pseudomonas putida |