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Literature summary extracted from

  • Schaer, A.; Sprecher, I.; Topakas, E.; Faulds, C.B.; Nystroem, L.
    Hydrolysis of nonpolar n-alkyl ferulates by feruloyl esterases (2016), J. Agric. Food Chem., 64, 8549-8554 .
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.73 0.0033
-
 n-decyl ferulate pH 7.0, 40°C rumen bacterium
3.1.1.73 0.008
-
 n-decyl ferulate pH 6.0, 40°C Aspergillus niger
3.1.1.73 0.01
-
 n-hexyl ferulate pH 6.0, 40°C Thermothelomyces thermophilus
3.1.1.73 0.027
-
 n-propyl ferulate pH 6.0, 40°C Thermothelomyces thermophilus
3.1.1.73 0.027
-
 n-hexyl ferulate pH 7.0, 40°C rumen bacterium
3.1.1.73 0.029
-
 n-hexyl ferulate pH 7.0, 40°C Acetivibrio thermocellus
3.1.1.73 0.04
-
 n-hexyl ferulate pH 6.0, 40°C Aspergillus niger
3.1.1.73 0.048
-
 ethyl ferulate pH 6.0, 40°C Thermothelomyces thermophilus
3.1.1.73 0.051
-
 methyl ferulate pH 6.0, 40°C Thermothelomyces thermophilus
3.1.1.73 0.081
-
 n-propyl ferulate pH 7.0, 40°C rumen bacterium
3.1.1.73 0.125
-
 n-decyl ferulate pH 7.0, 40°C Acetivibrio thermocellus
3.1.1.73 0.134
-
 methyl ferulate pH 7.0, 40°C rumen bacterium
3.1.1.73 0.149
-
 ethyl ferulate pH 7.0, 40°C rumen bacterium
3.1.1.73 0.245
-
 n-propyl ferulate pH 6.0, 40°C Aspergillus niger
3.1.1.73 0.611
-
 ethyl ferulate pH 6.0, 40°C Aspergillus niger
3.1.1.73 1.123
-
 methyl ferulate pH 6.0, 40°C Aspergillus niger
3.1.1.73 1.237
-
 n-propyl ferulate pH 7.0, 40°C Acetivibrio thermocellus
3.1.1.73 2.472
-
 methyl ferulate pH 7.0, 40°C Acetivibrio thermocellus
3.1.1.73 2.578
-
 ethyl ferulate pH 7.0, 40°C Acetivibrio thermocellus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Acetivibrio thermocellus
-
-
-
3.1.1.73 Aspergillus niger O42807
-
-
3.1.1.73 rumen bacterium
-
-
-
3.1.1.73 Thermothelomyces thermophilus G2QND5
-
-
3.1.1.73 Thermothelomyces thermophilus DSM 1799 G2QND5
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.73 commercial preparation
-
 rumen bacterium
-
3.1.1.73 commercial preparation
-
 Acetivibrio thermocellus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 ethyl ferulate + H2O
-
 rumen bacterium ferulate + ethanol
-
 ?
3.1.1.73 ethyl ferulate + H2O
-
 Acetivibrio thermocellus ferulate + ethanol
-
 ?
3.1.1.73 ethyl ferulate + H2O
-
 Aspergillus niger ferulate + ethanol
-
 ?
3.1.1.73 ethyl ferulate + H2O
-
 Thermothelomyces thermophilus ferulate + ethanol
-
 ?
3.1.1.73 ethyl ferulate + H2O
-
 Thermothelomyces thermophilus DSM 1799 ferulate + ethanol
-
 ?
3.1.1.73 methyl ferulate + H2O
-
 rumen bacterium ferulate + methanol
-
 ?
3.1.1.73 methyl ferulate + H2O
-
 Acetivibrio thermocellus ferulate + methanol
-
 ?
3.1.1.73 methyl ferulate + H2O
-
 Aspergillus niger ferulate + methanol
-
 ?
3.1.1.73 methyl ferulate + H2O
-
 Thermothelomyces thermophilus ferulate + methanol
-
 ?
3.1.1.73 methyl ferulate + H2O
-
 Thermothelomyces thermophilus DSM 1799 ferulate + methanol
-
 ?
3.1.1.73 n-decyl ferulate + H2O
-
 rumen bacterium ferulate + n-decanol
-
 ?
3.1.1.73 n-decyl ferulate + H2O
-
 Acetivibrio thermocellus ferulate + n-decanol
-
 ?
3.1.1.73 n-decyl ferulate + H2O
-
 Aspergillus niger ferulate + n-decanol
-
 ?
3.1.1.73 n-hexyl ferulate + H2O
-
 rumen bacterium ferulate + n-hexanol
-
 ?
3.1.1.73 n-hexyl ferulate + H2O
-
 Acetivibrio thermocellus ferulate + n-hexanol
-
 ?
3.1.1.73 n-hexyl ferulate + H2O
-
 Aspergillus niger ferulate + n-hexanol
-
 ?
3.1.1.73 n-hexyl ferulate + H2O
-
 Thermothelomyces thermophilus ferulate + n-hexanol
-
 ?
3.1.1.73 n-hexyl ferulate + H2O
-
 Thermothelomyces thermophilus DSM 1799 ferulate + n-hexanol
-
 ?
3.1.1.73 n-propyl ferulate + H2O
-
 rumen bacterium ferulate + n-propanol
-
 ?
3.1.1.73 n-propyl ferulate + H2O
-
 Acetivibrio thermocellus ferulate + n-propanol
-
 ?
3.1.1.73 n-propyl ferulate + H2O
-
 Aspergillus niger ferulate + n-propanol
-
 ?
3.1.1.73 n-propyl ferulate + H2O
-
 Thermothelomyces thermophilus ferulate + n-propanol
-
 ?
3.1.1.73 n-propyl ferulate + H2O
-
 Thermothelomyces thermophilus DSM 1799 ferulate + n-propanol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.73 Fae1A
-
 Thermothelomyces thermophilus
3.1.1.73 FAEA
-
 Aspergillus niger
3.1.1.73 feruloyl esterase B
-
 Thermothelomyces thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.73 0.2
-
 n-hexyl ferulate pH 7.0, 40°C Acetivibrio thermocellus
3.1.1.73 0.4
-
 n-decyl ferulate pH 7.0, 40°C Acetivibrio thermocellus
3.1.1.73 2.6
-
 n-decyl ferulate pH 7.0, 40°C rumen bacterium
3.1.1.73 3.2
-
 n-propyl ferulate pH 7.0, 40°C Acetivibrio thermocellus
3.1.1.73 4.6
-
 n-decyl ferulate pH 6.0, 40°C Aspergillus niger
3.1.1.73 5.7
-
 ethyl ferulate pH 7.0, 40°C Acetivibrio thermocellus
3.1.1.73 6.1
-
 n-hexyl ferulate pH 7.0, 40°C rumen bacterium
3.1.1.73 8
-
 methyl ferulate pH 7.0, 40°C Acetivibrio thermocellus
3.1.1.73 8.8
-
 methyl ferulate pH 6.0, 40°C Thermothelomyces thermophilus
3.1.1.73 8.9
-
 n-hexyl ferulate pH 6.0, 40°C Thermothelomyces thermophilus
3.1.1.73 9.8
-
 n-hexyl ferulate pH 6.0, 40°C Aspergillus niger
3.1.1.73 11.2
-
 ethyl ferulate pH 6.0, 40°C Thermothelomyces thermophilus
3.1.1.73 12.1
-
 n-propyl ferulate pH 6.0, 40°C Thermothelomyces thermophilus
3.1.1.73 29.4
-
 ethyl ferulate pH 6.0, 40°C Aspergillus niger
3.1.1.73 30.7
-
 ethyl ferulate pH 7.0, 40°C rumen bacterium
3.1.1.73 31.7
-
 n-propyl ferulate pH 7.0, 40°C rumen bacterium
3.1.1.73 32.9
-
 methyl ferulate pH 6.0, 40°C Aspergillus niger
3.1.1.73 33.5
-
 methyl ferulate pH 7.0, 40°C rumen bacterium
3.1.1.73 44.6
-
 n-propyl ferulate pH 6.0, 40°C Aspergillus niger

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.73 2.2
-
 ethyl ferulate pH 7.0, 40°C Acetivibrio thermocellus
3.1.1.73 2.6
-
 n-propyl ferulate pH 7.0, 40°C Acetivibrio thermocellus
3.1.1.73 3.2
-
 methyl ferulate pH 7.0, 40°C Acetivibrio thermocellus
3.1.1.73 3.2
-
 n-decyl ferulate pH 7.0, 40°C Acetivibrio thermocellus
3.1.1.73 5.6
-
 n-hexyl ferulate pH 7.0, 40°C Acetivibrio thermocellus
3.1.1.73 29
-
 methyl ferulate pH 6.0, 40°C Aspergillus niger
3.1.1.73 48
-
 ethyl ferulate pH 6.0, 40°C Aspergillus niger
3.1.1.73 173
-
 methyl ferulate pH 6.0, 40°C Thermothelomyces thermophilus
3.1.1.73 182
-
 n-propyl ferulate pH 6.0, 40°C Aspergillus niger
3.1.1.73 206
-
 ethyl ferulate pH 7.0, 40°C rumen bacterium
3.1.1.73 225
-
 n-hexyl ferulate pH 7.0, 40°C rumen bacterium
3.1.1.73 236
-
 ethyl ferulate pH 6.0, 40°C Thermothelomyces thermophilus
3.1.1.73 243
-
 n-hexyl ferulate pH 6.0, 40°C Aspergillus niger
3.1.1.73 250
-
 methyl ferulate pH 7.0, 40°C rumen bacterium
3.1.1.73 391
-
 n-propyl ferulate pH 7.0, 40°C rumen bacterium
3.1.1.73 452
-
 n-propyl ferulate pH 6.0, 40°C Thermothelomyces thermophilus
3.1.1.73 547
-
 n-decyl ferulate pH 6.0, 40°C Aspergillus niger
3.1.1.73 780
-
 n-decyl ferulate pH 7.0, 40°C rumen bacterium
3.1.1.73 906
-
 n-hexyl ferulate pH 6.0, 40°C Thermothelomyces thermophilus