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Literature summary extracted from

  • Eichler, A.; Gricman, Å.; Herter, S.; Kelly, P.; Turner, N.; Pleiss, J.; Flitsch, S.
    Enantioselective benzylic hydroxylation catalysed by P450 monooxygenases characterisation of a P450cam mutant library and molecular modelling (2016), ChemBioChem, 17, 426-432 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.15.1 recombinant expression of wild-type enzyme, mutant P450cam[Tyr96Phe]-RhFRed, and other enzyme mutants in Escherichia coli strain BL21(DE3) Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
1.14.15.1 L244F/V247L site-directed mutagenesis, the mutant exhibits moderate to high R-selectivity toward ethylmethylbenzene substrates and shows a narrow width of the binding pocket Pseudomonas putida
1.14.15.1 L244N/V247L site-directed mutagenesis, the mutant displays the highest S-selectivity toward substrates 1-ethyl-2-methylbenzene and 1-ethyl-3-methylbenzene, and low R-selectivity toward 1-ethyl-4-methylbenzene and shows a narrow width of the binding pocket Pseudomonas putida
1.14.15.1 M184V/T185F site-directed mutagenesis, the mutation introduces changes above the heme plane, prefers S-orientation of 1-ethyl-4-methylbenzene in the binding pocket of mutant, enantioselectivities of 1-ethyl-2-methylbenzene and 1-ethyl-3-methylbenzene are similar to the wild-type enzyme Pseudomonas putida
1.14.15.1 additional information enantioselectivity of a library of active-site mutants of chimeric P450cam-RhFRed towards the benzylic hydroxylation of structurally related regioisomers of ethylmethylbenzene, computational molecular modeling, overview Pseudomonas putida
1.14.15.1 Y96F site-directed mutagenesis Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.15.1 (+)-camphor + reduced putidaredoxin + O2 Pseudomonas putida
-
 (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.1 Pseudomonas putida P00183
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.1 (+)-camphor + reduced putidaredoxin + O2
-
 Pseudomonas putida (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
-
 ?
1.14.15.1 1-ethyl-2-methylbenzene + reduced putidaredoxin + O2 the S-enantiomer is preferred by the wild-type enzyme Pseudomonas putida ? + oxidized putidaredoxin + H2O
-
 ?
1.14.15.1 1-ethyl-3-methylbenzene + reduced putidaredoxin + O2 the S-enantiomer is preferred by the wild-type enzyme Pseudomonas putida ? + oxidized putidaredoxin + H2O
-
 ?
1.14.15.1 1-ethyl-4-methylbenzene + reduced putidaredoxin + O2 the S-enantiomer is preferred by the wild-type enzyme Pseudomonas putida ? + oxidized putidaredoxin + H2O
-
 ?
1.14.15.1 additional information enzyme P450cam produces benzyl alcohols with only moderate enantioselectivities, enantioselective benzylic hydroxylation catalysed by P450 monooxygenases, enantioselectivity of a library of active-site mutants of chimeric P450cam-RhFRed towards the benzylic hydroxylation of structurally related regioisomers of ethylmethylbenzene, molecular dynamics simulations and computational molecular modelling, overview Pseudomonas putida ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.15.1 CYP101
-
 Pseudomonas putida
1.14.15.1 P450cam
-
 Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.15.1 20
-
 assay at Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.15.1 7.2
-
 assay at Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.1 cytochrome P450
-
 Pseudomonas putida
1.14.15.1 NADH
-
 Pseudomonas putida
1.14.15.1 putidaredoxin
-
 Pseudomonas putida