EC Number | Application | Comment | Organism |
---|---|---|---|
4.2.1.41 | analysis | by establishing a pH-shift assay, an easy measurement method for 5-dehydro-4-deoxy-D-glucarate dehydratases/decarboxylase is developed. It can be used for measurements of the purified enzymes or using crude extracts. It is especially suitable as the method of choice within an engineering approach for further optimization of the enzyme | Comamonas testosteroni |
4.2.1.41 | analysis | by establishing a pH-shift assay, an easy measurement method for 5-keto-4-deoxy-D-glucarate dehydratases/decarboxylase is developed. It can be used for measurements of the purified enzymes or using crude extracts. It is especially suitable as the method of choice within an engineering approach for further optimization of the enzyme | Acinetobacter baylyi |
4.2.1.41 | analysis | by establishing a pH-shift assay, an easy measurement method for 5-keto-4-deoxy-D-glucarate dehydratases/decarboxylase is developed. It can be used for measurements of the purified enzymes or using crude extracts. It is especially suitable as the method of choice within an engineering approach for further optimization of the enzyme | Polaromonas naphthalenivorans CJ2 |
EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.1.41 | Escherichia coli ArcticExpress(DE3) | Acinetobacter baylyi |
4.2.1.41 | Escherichia coli ArcticExpress(DE3) | Comamonas testosteroni |
4.2.1.41 | Escherichia coli ArcticExpress(DE3) | Polaromonas naphthalenivorans CJ2 |
4.2.1.41 | expressed in Escherichia coli ArcticExpress(DE3) cells | Polaromonas naphthalenivorans |
4.2.1.41 | expressed in Escherichia coli ArcticExpress(DE3) cells | Comamonas testosteroni |
EC Number | General Stability | Organism |
---|---|---|
4.2.1.41 | NH4HCO3 at a concentration of 0.5 mM, maintains stability of the enzyme | Acinetobacter baylyi |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.41 | 1 | - |
5-dehydro-4-deoxy-D-glucarate | pH 7.0, 37°C | Acinetobacter baylyi | |
4.2.1.41 | 1.1 | - |
5-dehydro-4-deoxy-D-glucarate | pH 7.0, 37°C | Comamonas testosteroni | |
4.2.1.41 | 1.1 | - |
5-dehydro-4-deoxy-D-glucarate | pH 7.0, 37°C | Polaromonas naphthalenivorans CJ2 | |
4.2.1.41 | 1.1 | - |
5-dehydro-4-deoxy-D-glucarate | in 25 mM potassium phosphate buffer, at pH 7.0 and 37°C | Polaromonas naphthalenivorans | |
4.2.1.41 | 1.1 | - |
5-dehydro-4-deoxy-D-glucarate | in 25 mM potassium phosphate buffer, at pH 7.0 and 37°C | Comamonas testosteroni |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.41 | 5-dehydro-4-deoxy-D-glucarate | Polaromonas naphthalenivorans | - |
2,5-dioxopentanoate + H2O + CO2 | - |
? | |
4.2.1.41 | 5-dehydro-4-deoxy-D-glucarate | Comamonas testosteroni | - |
2,5-dioxopentanoate + H2O + CO2 | - |
? | |
4.2.1.41 | 5-dehydro-4-deoxy-D-glucarate | Comamonas testosteroni KF-1 | - |
2,5-dioxopentanoate + H2O + CO2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.41 | Acinetobacter baylyi | Q6FFQ1 | - |
- |
4.2.1.41 | Comamonas testosteroni | B7WZA5 | - |
- |
4.2.1.41 | Comamonas testosteroni KF-1 | B7WZA5 | - |
- |
4.2.1.41 | Polaromonas naphthalenivorans | A1VMN2 | - |
- |
4.2.1.41 | Polaromonas naphthalenivorans CJ2 | A1VMN2 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.1.41 | HisTrap column chromatography | Polaromonas naphthalenivorans |
4.2.1.41 | HisTrap column chromatography | Comamonas testosteroni |
EC Number | Storage Stability | Organism |
---|---|---|
4.2.1.41 | -20°C, 50 mM Tris-HCl, pH 7.5, 4 months, the enzyme remains stable | Polaromonas naphthalenivorans |
4.2.1.41 | -20°C, 50 mM Tris-HCl, pH 7.5, 4 months, the enzyme remains stable | Comamonas testosteroni |
4.2.1.41 | -80°C, 50 mM Tris-HCl, pH 7.5, 4 months, the enzyme remains stable | Polaromonas naphthalenivorans |
4.2.1.41 | -80°C, 50 mM Tris-HCl, pH 7.5, 4 months, the enzyme remains stable | Comamonas testosteroni |
4.2.1.41 | 8°C, 50 mM Tris-HCl, pH 7.5, 14 days, the enzyme remains stable | Polaromonas naphthalenivorans |
4.2.1.41 | 8°C, 50 mM Tris-HCl, pH 7.5, 14 days, the enzyme remains stable | Comamonas testosteroni |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.41 | 5-dehydro-4-deoxy-D-glucarate | - |
Acinetobacter baylyi | 2,5-dioxopentanoate + H2O + CO2 | - |
? | |
4.2.1.41 | 5-dehydro-4-deoxy-D-glucarate | - |
Polaromonas naphthalenivorans | 2,5-dioxopentanoate + H2O + CO2 | - |
? | |
4.2.1.41 | 5-dehydro-4-deoxy-D-glucarate | - |
Comamonas testosteroni | 2,5-dioxopentanoate + H2O + CO2 | - |
? | |
4.2.1.41 | 5-dehydro-4-deoxy-D-glucarate | - |
Polaromonas naphthalenivorans CJ2 | 2,5-dioxopentanoate + H2O + CO2 | - |
? | |
4.2.1.41 | 5-dehydro-4-deoxy-D-glucarate | - |
Comamonas testosteroni KF-1 | 2,5-dioxopentanoate + H2O + CO2 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.1.41 | 5-keto-4-deoxy-D-glucarate dehydratase/decarboxylase | - |
Polaromonas naphthalenivorans |
4.2.1.41 | 5-keto-4-deoxy-D-glucarate dehydratase/decarboxylase | - |
Comamonas testosteroni |
4.2.1.41 | 5-keto-4-deoxy-D-glucarate dehydratases/decarboxylase | - |
Acinetobacter baylyi |
4.2.1.41 | 5-keto-4-deoxy-D-glucarate dehydratases/decarboxylase | - |
Comamonas testosteroni |
4.2.1.41 | 5-keto-4-deoxy-D-glucarate dehydratases/decarboxylase | - |
Polaromonas naphthalenivorans CJ2 |
4.2.1.41 | KdgD | - |
Acinetobacter baylyi |
4.2.1.41 | KdgD | - |
Polaromonas naphthalenivorans |
4.2.1.41 | KdgD | - |
Comamonas testosteroni |
4.2.1.41 | KdgD | - |
Polaromonas naphthalenivorans CJ2 |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.1.41 | 37 | - |
assay at | Acinetobacter baylyi |
4.2.1.41 | 37 | - |
assay at | Comamonas testosteroni |
4.2.1.41 | 37 | - |
assay at | Polaromonas naphthalenivorans CJ2 |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.1.41 | 37 | - |
activity is stable over 150 h. NH4HCO3 (0.5 mM) shows no effect on stability | Comamonas testosteroni |
4.2.1.41 | 37 | - |
activity is stable over 150 h. NH4HCO3 (0.5 mM) shows no effect on stability | Polaromonas naphthalenivorans CJ2 |
4.2.1.41 | 37 | 65 | the enzyme is stable over 150 h at 37°C, whereas after 4 h at 65°C the initial activity decreases below 50% | Polaromonas naphthalenivorans |
4.2.1.41 | 37 | 65 | the enzyme is stable over 150 h at 37°C, whereas after 4 h at 65°C the initial activity decreases below 50% | Comamonas testosteroni |
4.2.1.41 | 65 | - |
after 4 h the initial activity decreases below 50%. T1/2: 2.79 h. NH4HCO3 (0.5 mM) shows no effect on stability | Comamonas testosteroni |
4.2.1.41 | 65 | - |
after 4 h the initial activity decreases below 50%. T1/2: 3.83 h. NH4HCO3 (0.5 mM) shows no effect on stability | Polaromonas naphthalenivorans CJ2 |
4.2.1.41 | 65 | - |
t1/2: 2.71 h in presence of 0.5 mM NH4HCO3 | Acinetobacter baylyi |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.41 | 1.7 | - |
5-dehydro-4-deoxy-D-glucarate | pH 7.0, 37°C | Polaromonas naphthalenivorans CJ2 | |
4.2.1.41 | 1.7 | - |
5-dehydro-4-deoxy-D-glucarate | in 25 mM potassium phosphate buffer, at pH 7.0 and 37°C | Polaromonas naphthalenivorans | |
4.2.1.41 | 3.1 | - |
5-dehydro-4-deoxy-D-glucarate | pH 7.0, 37°C | Comamonas testosteroni | |
4.2.1.41 | 3.1 | - |
5-dehydro-4-deoxy-D-glucarate | in 25 mM potassium phosphate buffer, at pH 7.0 and 37°C | Comamonas testosteroni | |
4.2.1.41 | 4.5 | - |
5-dehydro-4-deoxy-D-glucarate | pH 7.0, 37°C | Acinetobacter baylyi |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.1.41 | 7 | - |
assay at | Acinetobacter baylyi |
4.2.1.41 | 7 | - |
assay at | Comamonas testosteroni |
4.2.1.41 | 7 | - |
assay at | Polaromonas naphthalenivorans CJ2 |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.41 | 1.5 | - |
5-dehydro-4-deoxy-D-glucarate | pH 7.0, 37°C | Polaromonas naphthalenivorans CJ2 | |
4.2.1.41 | 1.5 | - |
5-dehydro-4-deoxy-D-glucarate | in 25 mM potassium phosphate buffer, at pH 7.0 and 37°C | Polaromonas naphthalenivorans | |
4.2.1.41 | 2.8 | - |
5-dehydro-4-deoxy-D-glucarate | pH 7.0, 37°C | Comamonas testosteroni | |
4.2.1.41 | 2.8 | - |
5-dehydro-4-deoxy-D-glucarate | in 25 mM potassium phosphate buffer, at pH 7.0 and 37°C | Comamonas testosteroni | |
4.2.1.41 | 4.5 | - |
5-dehydro-4-deoxy-D-glucarate | pH 7.0, 37°C | Acinetobacter baylyi |