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Literature summary extracted from

  • Tsuji, F.; Haneda, Y.
    Chemistry of the luciferases of Cypridina hilgendorfii and Apogon ellioti (2015), Biolumin. Progr., 2015, 137-149 .
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.12.6 Cypndina gamma-globulin Cypridina luciferase is completely inhibited by a rabbit antibody to Cypridina luciferase Vargula hilgendorfii
1.13.12.6 additional information Apogon luciferase is not inhibited by a rabbit antibody to Cypridina luciferase Apogon ellioti

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.12.6 additional information
-
 additional information Michaelis-Menten kinetics Vargula hilgendorfii
1.13.12.6 additional information
-
 additional information Michaelis-Menten kinetics Apogon ellioti

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.12.6 Cypridina luciferin + O2 Vargula hilgendorfii
-
 oxidized Cypridina luciferin + CO2 + hv
-
 ?
1.13.12.6 Cypridina luciferin + O2 Apogon ellioti
-
 oxidized Cypridina luciferin + CO2 + hv
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.6 Apogon ellioti
-
-
-
1.13.12.6 Vargula hilgendorfii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.12.6 native enzyme by fractional precipitation with acetone and ammonium sulfate, and dialysis, followed by gel filtration Vargula hilgendorfii
1.13.12.6 native enzyme from photogenic organs by fractional precipitation with acetone and ammonium sulfate, and dialysis, followed by gel filtration Apogon ellioti

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.12.6 photogenic organ
-
 Vargula hilgendorfii
-
1.13.12.6 photogenic organ
-
 Apogon ellioti
-

Storage Stability

EC Number Storage Stability Organism
1.13.12.6 Cypridina luciferase occasionally loses almost all of its activity when chromatographed through the 90-cm Sephadex column, irrespective of whether the column is newly poured or has been used for a period. The loss also occurs with both newly dissolved and old solutions of Cypridina luciferase Vargula hilgendorfii
1.13.12.6 purified native Apogon luciferase gradually loses activity on standing in the cold over a period of weeks Apogon ellioti

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.6 Cypridina luciferin + O2
-
 Vargula hilgendorfii oxidized Cypridina luciferin + CO2 + hv
-
 ?
1.13.12.6 Cypridina luciferin + O2
-
 Apogon ellioti oxidized Cypridina luciferin + CO2 + hv
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.12.6 Apogon luciferase
-
 Apogon ellioti
1.13.12.6 Apogon luciferase 1
-
 Apogon ellioti
1.13.12.6 Cypridina luciferase
-
 Vargula hilgendorfii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.12.6 0.0147
-
 Cypridina luciferin pH 6.8, 26°C Vargula hilgendorfii
1.13.12.6 0.0152
-
 Cypridina luciferin pH 6.8, 26°C Apogon ellioti