EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.1.116 | expression in Escherichia coli | [Clostridium] scindens |
1.3.1.116 | subcloned into an IPTG-inducible expression vector, pBaiH2.2. Escherichia coli DH5a cells transformed with pBaiH2.2 express 10fold higher levels of NADH:FOR upon induction with IPTG than does Eubacterium sp. VPI 12708 cells induced with cholic acid | [Clostridium] scindens |
1.5.1.36 | subcloned into an IPTG-inducible expression vector, pBaiH2.2. Escherichia coli DH5a cells transformed with pBaiH2.2 express 10fold higher levels of NADH:FOR upon induction with IPTG than did Eubacterium sp. VPI 12708 cells induced with cholic acid | [Clostridium] scindens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.116 | 1,10-phenanthroline | - |
[Clostridium] scindens | |
1.3.1.116 | Acriflavine | - |
[Clostridium] scindens | |
1.3.1.116 | CuCl2 | - |
[Clostridium] scindens | |
1.3.1.116 | EDTA | - |
[Clostridium] scindens | |
1.3.1.116 | EGTA | - |
[Clostridium] scindens | |
1.3.1.116 | HgCl2 | - |
[Clostridium] scindens | |
1.3.1.116 | additional information | iodoacetate and iodoacetamide show no inhibition | [Clostridium] scindens | |
1.3.1.116 | N-bromosuccinimide | - |
[Clostridium] scindens | |
1.3.1.116 | N-ethylmaleimide | - |
[Clostridium] scindens | |
1.3.1.116 | p-chloromercuriphenylsulfonic acid | - |
[Clostridium] scindens | |
1.3.1.116 | Sodium azide | - |
[Clostridium] scindens | |
1.3.1.116 | ZnCl2 | - |
[Clostridium] scindens | |
1.5.1.36 | 1,10-phenanthroline | - |
[Clostridium] scindens | |
1.5.1.36 | Acriflavine | - |
[Clostridium] scindens | |
1.5.1.36 | CuCl2 | - |
[Clostridium] scindens | |
1.5.1.36 | EDTA | - |
[Clostridium] scindens | |
1.5.1.36 | EGTA | - |
[Clostridium] scindens | |
1.5.1.36 | HgCl2 | - |
[Clostridium] scindens | |
1.5.1.36 | additional information | iodoacetate and iodoacetamide show no inhibition | [Clostridium] scindens | |
1.5.1.36 | N-bromosuccinimide | - |
[Clostridium] scindens | |
1.5.1.36 | N-ethylmaleimide | - |
[Clostridium] scindens | |
1.5.1.36 | p-chloromercuriphenylsulfonic acid | - |
[Clostridium] scindens | |
1.5.1.36 | Sodium azide | - |
[Clostridium] scindens | |
1.5.1.36 | ZnCl2 | - |
[Clostridium] scindens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.116 | copper | 1 mol of copper per mol of subunit | [Clostridium] scindens | |
1.3.1.116 | copper | contains 1 mol of copper per subunit | [Clostridium] scindens | |
1.3.1.116 | Iron | 2 mol of iron per mol of subunit | [Clostridium] scindens | |
1.3.1.116 | Iron | contains up to 2 mol of iron per subunit | [Clostridium] scindens | |
1.5.1.36 | copper | contains 1 mol of copper per subunit | [Clostridium] scindens | |
1.5.1.36 | Iron | contains up to 2 mol of iron per subunit | [Clostridium] scindens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.3.1.116 | 71400 | - |
SDS-PAGE | [Clostridium] scindens |
1.3.1.116 | 210000 | - |
gel filtration | [Clostridium] scindens |
1.3.1.116 | 210000 | - |
non-denaturing PAGE | [Clostridium] scindens |
1.5.1.36 | 210000 | - |
non-denaturing PAGE | [Clostridium] scindens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.1.116 | [Clostridium] scindens | P32370 | - |
- |
1.3.1.116 | [Clostridium] scindens JCM 10418 | P32370 | - |
- |
1.3.1.116 | [Clostridium] scindens VPI 12708 | P32370 | - |
- |
1.5.1.36 | [Clostridium] scindens | P32370 | - |
- |
1.5.1.36 | [Clostridium] scindens VPI 12708 | P32370 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.3.1.116 | - |
[Clostridium] scindens |
1.3.1.116 | recombinant protein | [Clostridium] scindens |
1.5.1.36 | - |
[Clostridium] scindens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.116 | additional information | the enzyme reduces synthetic quinones, dyes, flavins, and O2 with NADH as the electron donor, but does not reduce disulfide compounds, various unsaturated bile acids, cytochrome c and physiological quinones | [Clostridium] scindens | ? | - |
? | |
1.3.1.116 | additional information | the enzyme reduces synthetic quinones, dyes, flavins, and O2 with NADH as the electron donor, but does not reduce disulfide compounds, various unsaturated bile acids, cytochrome c and physiological quinones | [Clostridium] scindens JCM 10418 | ? | - |
? | |
1.3.1.116 | additional information | the enzyme reduces synthetic quinones, dyes, flavins, and O2 with NADH as the electron donor, but does not reduce disulfide compounds, various unsaturated bile acids, cytochrome c and physiological quinones | [Clostridium] scindens VPI 12708 | ? | - |
? | |
1.5.1.36 | reduced flavin + NAD+ | - |
[Clostridium] scindens | flavin + NADH + H+ | - |
? | |
1.5.1.36 | reduced flavin + NAD+ | - |
[Clostridium] scindens JCM 10418 | flavin + NADH + H+ | - |
? | |
1.5.1.36 | reduced flavin + NAD+ | - |
[Clostridium] scindens VPI 12708 | flavin + NADH + H+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.3.1.116 | homotrimer | 3 * 71400, SDS-PAGE | [Clostridium] scindens |
1.3.1.116 | trimer | 3 * 72000, SDS-PAGE | [Clostridium] scindens |
1.5.1.36 | homotrimer | 3 * 71400 | [Clostridium] scindens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.1.116 | BaiH | - |
[Clostridium] scindens |
1.5.1.36 | BaiH | - |
[Clostridium] scindens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.3.1.116 | 40 | - |
- |
[Clostridium] scindens |
1.5.1.36 | 40 | - |
- |
[Clostridium] scindens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.3.1.116 | 6.8 | - |
- |
[Clostridium] scindens |
1.5.1.36 | 6.8 | - |
- |
[Clostridium] scindens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.116 | FAD | 1 mol of FAD per mol of subunit | [Clostridium] scindens | |
1.3.1.116 | FAD | contains 1 mol of FAD | [Clostridium] scindens | |
1.3.1.116 | NADH | - |
[Clostridium] scindens | |
1.5.1.36 | FAD | contains 1 mol of FAD | [Clostridium] scindens | |
1.5.1.36 | NADH | - |
[Clostridium] scindens |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.3.1.116 | [Clostridium] scindens | isoelectric focusing | - |
6 |
1.5.1.36 | [Clostridium] scindens | isoelectric focusing | - |
6 |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.3.1.116 | physiological function | enzyme reduces synthetic quinones, dyes, flavins, and O2 with NADH as the electron donor. Addition of purified enzyme to Clostridium scindens cell extracts alters the balance of oxidized and reduced bile acid intermediates produced during cholic acid 7alpha-dehydroxylation | [Clostridium] scindens |