Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Waki, T.; Yoo, D.; Fujino, N.; Mameda, R.; Denessiouk, K.; Yamashita, S.; Motohashi, R.; Akashi, T.; Aoki, T.; Ayabe, S.; Takahashi, S.; Nakayama, T.
    Identification of protein-protein interactions of isoflavonoid biosynthetic enzymes with 2-hydroxyisoflavanone synthase in soybean (Glycine max (L.) Merr.) (2016), Biochem. Biophys. Res. Commun., 469, 546-551 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.87 expression in Saccharomyces cerevisiae Glycine max
1.14.14.87 gene ifs1, protein-protein interaction analysis using the split-ubiquitin system, in planta by BiFC, and the yeast two-hybrid system in Saccharomyces cerevisiae. No appreciable yeast growth is detected with any combinations of proteins under the assay conditions Glycine max

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.87 endoplasmic reticulum
-
 Glycine max 5783
-
1.14.14.87 microsome
-
 Glycine max
-
-

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.87 Glycine max Q9M6B9
-
-

Synonyms

EC Number Synonyms Comment Organism
1.14.14.87 2-hydroxyisoflavanone synthase 1
-
 Glycine max
1.14.14.87 CYP93C
-
 Glycine max
1.14.14.87 GmIFS
-
 Glycine max
1.14.14.87 GmIFS1
-
 Glycine max
1.14.14.87 IFS1
-
 Glycine max

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.87 cytochrome P450 CYP93C Glycine max

General Information

EC Number General Information Comment Organism
1.14.14.87 metabolism (2S)-flavanones undergo 2-hydroxylation catalyzed by 2-hydroxyisoflavanone synthase (GmIFS), a microsomal cytochrome P450 enzyme (CYP93C), to produce 2-hydroxyisoflavanones pathway of isoflavone biosynthesis in soybean. Analysis of binary protein-protein interactions of 2-hydroxyisoflavanone synthase 1 (GmIFS1), a P450 (CYP93C), with cytoplasmic enzymes involved in isoflavone biosynthesis in soybean, by split-ubiquitin system. Identification of binary interactions between GmIFS1 and chalcone synthase 1 (GmCHS1), and between GmIFS1 and chalcone isomerases (GmCHIs), the interaction of GmCHS1 and GmCHIs with GmIFS1 takes place on endoplasmic reticulum on which GmIFS1 is located Glycine max
1.14.14.87 physiological function enzyme shows binary interactions with cytoplasmic chalcone synthase 1 and chalcone isomerases CHI, both in recombinant Saccharomyces cerevisiae and in planta Glycine max