Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Mugo, A.N.; Kobayashi, J.; Mikami, B.; Yoshikane, Y.; Yagi, T.; Ohnishi, K.
    Crystal structure of 5-formyl-3-hydroxy-2-methylpyridine 4-carboxylic acid 5-dehydrogenase, an NAD+-dependent dismutase from Mesorhizobium loti (2015), Biochem. Biophys. Res. Commun., 456, 35-40 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.99.42 expression in Escherichia coli Mesorhizobium japonicum
1.2.1.100 expression in Escherichia coli Mesorhizobium japonicum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.99.42 structure determined by molecular replacement, to 1. 55 A resolution. Residues Ser116, His137 and Glu149 are connected by a hydrogen bonding network forming a catalytic triad Mesorhizobium japonicum
1.2.1.100 structure determined by molecular replacement, to 1. 55 A resolution. Residues Ser116, His137 and Glu149 are connected by a hydrogen bonding network forming a catalytic triad Mesorhizobium japonicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.100 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate + NAD+ + H2O Mesorhizobium japonicum
-
 3-hydroxy-2-methylpyridine-4,5-dicarboxylate + NADH + H+
-
 ?
1.2.1.100 additional information Mesorhizobium japonicum enzyme catalyzes oxidation of the hemiacetal form of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate to 3-hydroxy-2-methylpyridine-4,5-dicarboxylate with NAD+ and reduction of an aldehyde form of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate to 4-pyridoxic acid with NADH, i.e. reaction of 4-pyridoxic acid dehydrogenase. The Ser-His-Glu catalytic triad facilitates the two-way reactions. Ser116 assists protonation of His137 to drive the reduction reaction. His137 acts as a catalytic base to abstract a proton during oxidation. Glu149 likely neutralizes the positive charge on His137 after the deprotonation of the substrate ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.42 Mesorhizobium japonicum Q988C8 enzyme additionally catalyzes the reaction of EC 1.2.1.100
-
1.2.1.100 Mesorhizobium japonicum Q988C8 enzyme additionally catalyzes the reaction of 4-pyridoxic acid dehydrogenase
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.42 4-pyridoxate + NAD+
-
 Mesorhizobium japonicum 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate + NADH + H+
-
 ?
1.1.99.42 additional information enzyme catalyzes oxidation of the hemiacetal form of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate to 3-hydroxy-2-methylpyridine-4,5-dicarboxylate with NAD+, reaction of EC 1.2.1.100, and reduction of an aldehyde form of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate to 4-pyridoxic acid with NADH. The Ser-His-Glu catalytic triad facilitates the two-way reactions. Ser116 assists protonation of His137 to drive the reduction reaction. His137 acts as a catalytic base to abstract a proton during oxidation. Glu149 likely neutralizes the positive charge on His137 after the deprotonation of the substrate Mesorhizobium japonicum ?
-
 ?
1.2.1.100 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate + NAD+ + H2O
-
 Mesorhizobium japonicum 3-hydroxy-2-methylpyridine-4,5-dicarboxylate + NADH + H+
-
 ?
1.2.1.100 additional information enzyme catalyzes oxidation of the hemiacetal form of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate to 3-hydroxy-2-methylpyridine-4,5-dicarboxylate with NAD+ and reduction of an aldehyde form of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate to 4-pyridoxic acid with NADH, i.e. reaction of 4-pyridoxic acid dehydrogenase. The Ser-His-Glu catalytic triad facilitates the two-way reactions. Ser116 assists protonation of His137 to drive the reduction reaction. His137 acts as a catalytic base to abstract a proton during oxidation. Glu149 likely neutralizes the positive charge on His137 after the deprotonation of the substrate Mesorhizobium japonicum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.99.42 mlr6793
-
 Mesorhizobium japonicum
1.2.1.100 mlr6793
-
 Mesorhizobium japonicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.99.42 8
-
-
 Mesorhizobium japonicum
1.2.1.100 8
-
-
 Mesorhizobium japonicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.42 NAD+
-
 Mesorhizobium japonicum