EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.14.9 | - |
Acinetobacter baumannii |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.14.9 | R263A | mutation of oxygenase component, 72% hydroxylation efficiency of phydroxyphenylacetate, 7% hydroxylation of tyramine | Acinetobacter baumannii |
1.14.14.9 | R263D | mutation of oxygenase component, variant can catalyze hydroxylation of tyramine to form dopamine with the highest yield (57%) while maintaining 86% hydroxylation efficiency of phydroxyphenylacetate | Acinetobacter baumannii |
1.14.14.9 | R263E | mutation of oxygenase component, 73% hydroxylation efficiency of phydroxyphenylacetate, no hydroxylation of tyramine | Acinetobacter baumannii |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.9 | Acinetobacter baumannii | Q6Q272 | oxygenase component C2 | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.9 | 4-hydroxyphenylacetate + FMNH + O2 | - |
Acinetobacter baumannii | 3,4-dihydroxyphenylacetate + FMN + H2O | - |
? | |
1.14.14.9 | octopamine + FMNH + O2 | no substrate of wild-type or mutant R263E, R263D, R263A, but substrate of mutant R263D/Y398D | Acinetobacter baumannii | norepinephrine + FMN + H2O | - |
? | |
1.14.14.9 | tyramine + FMNH + O2 | no substrate of wild-type or mutant R263E, but substrate of mutants R263D and R263A | Acinetobacter baumannii | dopamine + FMN + H2O | - |
? |