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Literature summary extracted from

  • Kitaoka, N.; Wu, Y.; Xu, M.; Peters, R.J.
    Optimization of recombinant expression enables discovery of novel cytochrome P450 activity in rice diterpenoid biosynthesis (2015), Appl. Microbiol. Biotechnol., 99, 7549-7558 .
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.68 0.027
-
 9beta-pimara-7,15-diene pH 7.5, 32°C Oryza sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.76 ent-isokauren-2beta-ol + O2 + [reduced NADPH-hemoprotein reductase] Oryza sativa Japonica Group
-
 ent-isokaurene-2beta,3beta-diol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.76 ent-isokaurene + O2 + [reduced NADPH-hemoprotein reductase] Oryza sativa Japonica Group
-
 ent-isokauren-2beta-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.76 additional information Oryza sativa Japonica Group CYP71Z6 catalyzes the production of 2alpha,3alpha-dihydroxy-ent-isokaurene via 2alpha-hydroxy-ent-isokaurene ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.68 Oryza sativa Q0DBF4
-
-
1.14.14.69 Oryza sativa Japonica Group Q6YV88
-
-
1.14.14.76 Oryza sativa Japonica Group A3A871
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.68 9beta-pimara-7,15-diene + [reduced NADPH-hemoprotein reductase] + O2
-
 Oryza sativa 9beta-pimara-7,15-diene-3beta-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.69 ent-cassa-12,15-dien-2-one + [reduced NADPH-hemoprotein reductase] + O2
-
 Oryza sativa Japonica Group ent-3beta-hydroxycassa-12,15-dien-2-one + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.69 ent-cassa-12,15-dien-2beta-ol + [reduced NADPH-hemoprotein reductase] + O2
-
 Oryza sativa Japonica Group ent-cassa-12,15-dien-2-one + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
1.14.14.69 ent-cassa-12,15-dien-2beta-ol + [reduced NADPH-hemoprotein reductase] + O2
-
 Oryza sativa Japonica Group ent-cassa-12,15-diene-2beta,3beta-diol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.69 ent-cassa-12,15-diene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
 Oryza sativa Japonica Group ent-3beta-hydroxycassa-12,15-dien-2-one + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
 ?
1.14.14.69 ent-cassa-12,15-diene + [reduced NADPH-hemoprotein reductase] + O2
-
 Oryza sativa Japonica Group ent-cassa-12,15-dien-2beta-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.69 ent-cassa-12,15-diene-2beta,3beta-diol + [reduced NADPH-hemoprotein reductase] + O2
-
 Oryza sativa Japonica Group ent-3beta-hydroxycassa-12,15-dien-2-one + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
1.14.14.69 additional information enzyme catalyzes the production of 3alpha-hydroxy-ent-cassadien-2-one via 2alpha-hydroxy-ent-cassadiene and ent-cassadien-2-one Oryza sativa Japonica Group ?
-
 ?
1.14.14.76 ent-isokauren-2beta-ol + O2 + [reduced NADPH-hemoprotein reductase]
-
 Oryza sativa Japonica Group ent-isokaurene-2beta,3beta-diol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.76 ent-isokaurene + O2 + [reduced NADPH-hemoprotein reductase]
-
 Oryza sativa Japonica Group ent-isokauren-2beta-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.76 additional information CYP71Z6 catalyzes the production of 2alpha,3alpha-dihydroxy-ent-isokaurene via 2alpha-hydroxy-ent-isokaurene Oryza sativa Japonica Group ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.68 CYP701A8
-
 Oryza sativa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.14.68 32
-
 assay at Oryza sativa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.14.68 1.4
-
 9beta-pimara-7,15-diene pH 7.5, 32°C Oryza sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.68 7.5
-
 assay at Oryza sativa

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.68 cytochrome P450
-
 Oryza sativa

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.14.14.68 52
-
 9beta-pimara-7,15-diene pH 7.5, 32°C Oryza sativa