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Literature summary extracted from
- Cloning and heterologous expression of isocyanide biosynthetic genes from environmental DNA (2005), Angew. Chem. Int. Ed. Engl., 44, 7063-7065 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.1.99.25 | heterologous expression of DNA extracted directly from environmental samples (environmental DNA, eDNA) in Escherichia coli | uncultured organism |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.99.25 | L-tryptophan + D-ribulose 5-phosphate | uncultured organism | the enzyme catalyzes the biosynthesis of the isocyanide-containing antibiotic(2S)-3-(1H-indol-3-yl)-2-isocyanopropanoate (i.e. L-tryptophan isonitrile) | (2S)-3-(1H-indol-3-yl)-2-isocyanopropanoate + hydroxyacetone + formaldehyde + phosphate + H2O | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.20.12 | uncultured organism | Q108L2 | - |
- |
| 4.1.99.25 | uncultured organism | Q108L3 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.1.99.25 | - |
uncultured organism |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.20.12 | (2S)-3-(1H-indol-3-yl)-2-isocyanopropanoate + 2-oxoglutarate + O2 | - |
uncultured organism | 3-[(E)-2-isocyanoethenyl]-1H-indole + succinate + 2 CO2 + H2O | - |
? | |
| 4.1.99.25 | L-tryptophan + D-ribulose 5-phosphate | the enzyme catalyzes the biosynthesis of the isocyanide-containing antibiotic(2S)-3-(1H-indol-3-yl)-2-isocyanopropanoate (i.e. L-tryptophan isonitrile) | uncultured organism | (2S)-3-(1H-indol-3-yl)-2-isocyanopropanoate + hydroxyacetone + formaldehyde + phosphate + H2O | - |
? | |
| 4.1.99.25 | L-tryptophan + D-ribulose 5-phosphate | (2S)-3-(1H-indol-3-yl)-2-isocyanopropanoate i.e. L-tryptophan isonitrile, hydroxyacetone i.e. 1-hydroxypropan-2-one | uncultured organism | (2S)-3-(1H-indol-3-yl)-2-isocyanopropanoate + hydroxyacetone + formaldehyde + phosphate + H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.20.12 | isnB | - |
uncultured organism |
| 4.1.99.25 | isnA | - |
uncultured organism |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.14.20.12 | physiological function | presence of isonitrile synthase IsnA and IsnB is necessary and sufficient to generate 3-[(E)-2-isocyanoethenyl]-1H-indole from L-Trp and ribulose 5-phosphate. The isocyanide group in (2S)-3-(1H-indol-3-yl)-2-isocyanopropanoate is formed in a single enzymatic step on IsnA and the isocyanide-containing intermediate is oxidatively decarboxylated by IsnB to give 3-[(E)-2-isocyanoethenyl]-1H-indole. Both the nitrogen atoms in 3-[(E)-2-isocyanoethenyl]-1H-indole, including the isocyanide nitrogen atom, are derived from tryptophan | uncultured organism |
| 4.1.99.25 | metabolism | the enzyme catalyzes the biosynthesis of the isocyanide-containing antibiotic(2S)-3-(1H-indol-3-yl)-2-isocyanopropanoate | uncultured organism |
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