EC Number | Cloned (Comment) | Organism |
---|---|---|
6.1.1.27 | expressed in Escherichia coli B834(DE3) cells | Methanocaldococcus jannaschii |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.5.1.73 | crystal structure of a subcomplex of the transsulfursome with tRNACys (SepCysS-SepCysE-tRNACys), which is involved in the second reaction step of the indirect pathway, to 2.6 A resolution. Space group P6522, with unit-cell parameters a = b = 107.2, c = 551.1 A. Two heterodimers of a synthase SepCysS dimer, an enhancer SepCysE dimer and one tRNACys are observed in the asymmetric unit | Methanocaldococcus jannaschii |
6.1.1.27 | sitting drop vapor diffusion method, using 0.1 M MES pH 5.3, 2.37 M ammonium sulfate | Methanocaldococcus jannaschii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.27 | ATP + O-phospho-L-serine + tRNACys | Methanocaldococcus jannaschii | - |
AMP + diphosphate + O-phospho-L-seryl-tRNACys | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.73 | Methanocaldococcus jannaschii | Q59072 | - |
- |
2.5.1.73 | Methanocaldococcus jannaschii DSM 2661 | Q59072 | - |
- |
6.1.1.27 | Methanocaldococcus jannaschii | Q59054 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.1.1.27 | HisTrap column chromatography, HiTrap heparin column chromatography, and Superdex 200 gel filtration | Methanocaldococcus jannaschii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.27 | ATP + O-phospho-L-serine + tRNACys | - |
Methanocaldococcus jannaschii | AMP + diphosphate + O-phospho-L-seryl-tRNACys | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.73 | SepCysS | - |
Methanocaldococcus jannaschii |
6.1.1.27 | CysRS | - |
Methanocaldococcus jannaschii |
6.1.1.27 | Cysteinyl-tRNA synthetase | - |
Methanocaldococcus jannaschii |
6.1.1.27 | O-phosphoseryl-tRNA synthetase | - |
Methanocaldococcus jannaschii |
6.1.1.27 | SepRS | - |
Methanocaldococcus jannaschii |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
6.1.1.27 | ATP | - |
Methanocaldococcus jannaschii |