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Literature summary extracted from

  • Winzer, T.; Kern, M.; King, A.J.; Larson, T.R.; Teodor, R.I.; Donninger, S.L.; Li, Y.; Dowle, A.A.; Cartwright, J.; Bates, R.; Ashford, D.; Thomas, J.; Walker, C.; Bowser, T.A.; Graham, I.A.
    Plant science. Morphinan biosynthesis in opium poppy requires a P450-oxidoreductase fusion protein (2015), Science, 349, 309-312 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.27 DNA and amino acid sequence determination and analysis, the genetic locus, designated STORR [(S)- to (R)-reticuline] encodes both cytochrome P450 and oxidoreductase modules Papaver somniferum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.19.54 0.013
-
 (S)-reticuline Cyp82Y2 module, pH not specified in the publication, temperature not specified in the publication Papaver somniferum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.19.54 microsome
-
 Papaver somniferum
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.27 1,2-dehydroreticulinium + NADPH + H+ Papaver somniferum
-
 (R)-reticuline + NADP+
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.27 Papaver somniferum P0DKI7
-
-
1.14.19.54 Papaver somniferum P0DKI7 bifunctional protein, the P450 module is responsible for the conversion of (S)-reticuline to 1,2-dehydroreticuline, whereas the oxidoreductase module converts 1,2-dehydroreticuline to (R)-reticuline rather than functioning as a P450 redox partner
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.27 1,2-dehydroreticulinium + NADPH + H+
-
 Papaver somniferum (R)-reticuline + NADP+
-
 ir
1.14.19.54 (S)-reticuline + [reduced NADPH-hemoprotein reductase] + O2
-
 Papaver somniferum 1,2-dehydroreticuline + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.1.27 STORR
-
 Papaver somniferum
1.14.19.54 STORR
-
 Papaver somniferum

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.27 NADPH
-
 Papaver somniferum
1.14.19.54 cytochrome P450
-
 Papaver somniferum

General Information

EC Number General Information Comment Organism
1.5.1.27 evolution the enzyme belongs to the aldo-keto reductase family Papaver somniferum
1.5.1.27 physiological function direction of metabolites to morphinan biosynthesis requires isomerization of (S)- to (R)-reticuline. The P450 module of the bifunctional enzyme is responsible for the conversion of (S)-reticuline to 1,2-dehydroreticuline, whereas the oxidoreductase module converts 1,2-dehydroreticuline to (R)-reticuline rather than functioning as a P450 redox partner. Proteomic analysis confirms that these two modules are contained on a single polypeptide in vivo Papaver somniferum