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Literature summary extracted from

  • Zhan, T.; Zhang, K.; Chen, Y.; Lin, Y.; Wu, G.; Zhang, L.; Yao, P.; Shao, Z.; Liu, Z.
    Improving glyphosate oxidation activity of glycine oxidase from Bacillus cereus by directed evolution (2013), PLoS ONE, 8, e79175 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.19 expressed in Escherichia coli BL21(DE3) cells Bacillus cereus

Protein Variants

EC Number Protein Variants Comment Organism
1.4.3.19 BG51R/G60S/I198V/E357G the mutant shows no activity with glycine and high activity with glyphosate Bacillus cereus
1.4.3.19 BG51R/G60S/S210P/M267T the mutant shows no activity with glycine and activity with glyphosate Bacillus cereus
1.4.3.19 D60G the mutant shows activity with glycine and very low activity with glyphosate Bacillus cereus
1.4.3.19 G51R the mutant shows no activity with glycine and very low activity with glyphosate Bacillus cereus
1.4.3.19 G51R/D60G the mutant shows no activity with glycine and activity with glyphosate Bacillus cereus
1.4.3.19 G51R/D60G/K133R/V262I the mutant shows no activity with glycine and activity with glyphosate Bacillus cereus
1.4.3.19 G51R/D60G/T118A/K133R the mutant shows no activity with glycine and activity with glyphosate Bacillus cereus
1.4.3.19 G51R/D60G/T118A/K133R/I284L the mutant shows no activity with glycine and very high activity with glyphosate Bacillus cereus
1.4.3.19 G51R/G60S/D121G/S122P/H164Y/M267T the mutant shows no activity with glycine and high activity with glyphosate Bacillus cereus
1.4.3.19 G51R/G60S/K133R/S210P/R250G/V262I the mutant shows no activity with glycine and high activity with glyphosate Bacillus cereus
1.4.3.19 G51R/G60S/T118A/K133R/I198V/V262I/I284L/L307S/E357G the mutant shows no activity with glycine and high activity with glyphosate Bacillus cereus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.19 0.53
-
 glyphosate mutant enzyme G51R/G60S/T118A/K133R/I198V/V262I/I284L/L307S/E357G, at pH 8.5 and 60°C Bacillus cereus
1.4.3.19 0.57
-
 glyphosate mutant enzyme G51R/D60G/T118A/K133R/I284L, at pH 8.5 and 60°C Bacillus cereus
1.4.3.19 0.99
-
 glycine mutant enzyme D60G, at pH 8.5 and 60°C Bacillus cereus
1.4.3.19 1.04
-
 glycine wild type enzyme, at pH 8.5 and 60°C Bacillus cereus
1.4.3.19 1.37
-
 glyphosate mutant enzyme G51R/G60S/D121G/S122P/H164Y/M267T, at pH 8.5 and 60°C Bacillus cereus
1.4.3.19 1.69
-
 glyphosate mutant enzyme G51R/G60S/K133R/S210P/R250G/V262I, at pH 8.5 and 60°C Bacillus cereus
1.4.3.19 2.17
-
 glyphosate mutant enzyme G51R/D60G/T118A/K133R, at pH 8.5 and 60°C Bacillus cereus
1.4.3.19 2.45
-
 glyphosate mutant enzyme G51R/D60G, at pH 8.5 and 60°C Bacillus cereus
1.4.3.19 2.77
-
 glyphosate mutant enzyme G51R/D60G/K133R/V262I, at pH 8.5 and 60°C Bacillus cereus
1.4.3.19 3.8
-
 glyphosate mutant enzyme BG51R/G60S/I198V/E357G, at pH 8.5 and 60°C Bacillus cereus
1.4.3.19 4.37
-
 glyphosate mutant enzyme BG51R/G60S/S210P/M267T, at pH 8.5 and 60°C Bacillus cereus
1.4.3.19 8.29
-
 glyphosate mutant enzyme G51R, at pH 8.5 and 60°C Bacillus cereus
1.4.3.19 18.88
-
 glyphosate mutant enzyme D60G, at pH 8.5 and 60°C Bacillus cereus
1.4.3.19 41.55
-
 glycine mutant enzyme G51R/G60S/T118A/K133R/I198V/V262I/I284L/L307S/E357G, at pH 8.5 and 60°C Bacillus cereus
1.4.3.19 41.64
-
 glycine mutant enzyme G51R/D60G/T118A/K133R/I284L, at pH 8.5 and 60°C Bacillus cereus
1.4.3.19 54.6
-
 glycine mutant enzyme G51R, at pH 8.5 and 60°C Bacillus cereus
1.4.3.19 58.5
-
 glycine mutant enzyme G51R/D60G, at pH 8.5 and 60°C Bacillus cereus
1.4.3.19 80.43
-
 glycine mutant enzyme G51R/G60S/D121G/S122P/H164Y/M267T, at pH 8.5 and 60°C Bacillus cereus
1.4.3.19 84.79
-
 glyphosate wild type enzyme, at pH 8.5 and 60°C Bacillus cereus
1.4.3.19 92.5
-
 glycine mutant enzyme G51R/D60G/T118A/K133R, at pH 8.5 and 60°C Bacillus cereus
1.4.3.19 101.8
-
 glycine mutant enzyme BG51R/G60S/I198V/E357G, at pH 8.5 and 60°C Bacillus cereus
1.4.3.19 105.6
-
 glycine mutant enzyme G51R/D60G/K133R/V262I, at pH 8.5 and 60°C Bacillus cereus
1.4.3.19 134.4
-
 glycine mutant enzyme BG51R/G60S/S210P/M267T, at pH 8.5 and 60°C Bacillus cereus
1.4.3.19 138.1
-
 glycine mutant enzyme G51R/G60S/K133R/S210P/R250G/V262I, at pH 8.5 and 60°C Bacillus cereus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.19 2-iminoacetate + H2O Bacillus cereus
-
 glyoxylate + NH3
-
 ?
1.4.3.19 glycine + H2O + O2 Bacillus cereus
-
 glyoxylate + NH3 + H2O2
-
 ?
1.4.3.19 glycine + O2 Bacillus cereus
-
 2-iminoacetate + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.19 Bacillus cereus L7T8Y9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.19 GST-bind resin column chromatography Bacillus cereus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.19 2-iminoacetate + H2O
-
 Bacillus cereus glyoxylate + NH3
-
 ?
1.4.3.19 glycine + H2O + O2
-
 Bacillus cereus glyoxylate + NH3 + H2O2
-
 ?
1.4.3.19 glycine + O2
-
 Bacillus cereus 2-iminoacetate + H2O2
-
 ?
1.4.3.19 glyphosate + H2O + O2 the wild type enzyme shows very low activity with glyphosate Bacillus cereus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.4.3.19 GOX
-
 Bacillus cereus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.3.19 60
-
-
 Bacillus cereus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.3.19
-
 50 the enzyme remains stable between 0 and 50°C after 6 h incubation Bacillus cereus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.19 0.007
-
 glycine mutant enzyme G51R/D60G, at pH 8.5 and 60°C Bacillus cereus
1.4.3.19 0.019
-
 glycine mutant enzyme G51R, at pH 8.5 and 60°C Bacillus cereus
1.4.3.19 0.0225
-
 glycine mutant enzyme G51R/D60G/T118A/K133R, at pH 8.5 and 60°C Bacillus cereus
1.4.3.19 0.031
-
 glycine mutant enzyme G51R/D60G/K133R/V262I, at pH 8.5 and 60°C Bacillus cereus
1.4.3.19 0.038
-
 glycine mutant enzyme G51R/D60G/T118A/K133R/I284L, at pH 8.5 and 60°C Bacillus cereus
1.4.3.19 0.046
-
 glyphosate mutant enzyme G51R/D60G, at pH 8.5 and 60°C Bacillus cereus
1.4.3.19 0.049
-
 glyphosate mutant enzyme G51R, at pH 8.5 and 60°C Bacillus cereus
1.4.3.19 0.069
-
 glyphosate mutant enzyme G51R/D60G/T118A/K133R, at pH 8.5 and 60°C Bacillus cereus
1.4.3.19 0.076
-
 glycine mutant enzyme D60G, at pH 8.5 and 60°C Bacillus cereus
1.4.3.19 0.077
-
 glyphosate mutant enzyme G51R/D60G/T118A/K133R/I284L, at pH 8.5 and 60°C Bacillus cereus
1.4.3.19 0.09
-
 glycine mutant enzyme BG51R/G60S/S210P/M267T, at pH 8.5 and 60°C Bacillus cereus
1.4.3.19 0.09
-
 glycine mutant enzyme G51R/G60S/T118A/K133R/I198V/V262I/I284L/L307S/E357G, at pH 8.5 and 60°C Bacillus cereus
1.4.3.19 0.095
-
 glycine mutant enzyme G51R/G60S/D121G/S122P/H164Y/M267T, at pH 8.5 and 60°C Bacillus cereus
1.4.3.19 0.095
-
 glyphosate wild type enzyme, at pH 8.5 and 60°C Bacillus cereus
1.4.3.19 0.12
-
 glyphosate mutant enzyme D60G, at pH 8.5 and 60°C Bacillus cereus
1.4.3.19 0.121
-
 glyphosate mutant enzyme BG51R/G60S/S210P/M267T, at pH 8.5 and 60°C Bacillus cereus
1.4.3.19 0.14
-
 glycine wild type enzyme, at pH 8.5 and 60°C Bacillus cereus
1.4.3.19 0.169
-
 glycine mutant enzyme G51R/G60S/K133R/S210P/R250G/V262I, at pH 8.5 and 60°C Bacillus cereus
1.4.3.19 0.195
-
 glyphosate mutant enzyme G51R/G60S/T118A/K133R/I198V/V262I/I284L/L307S/E357G, at pH 8.5 and 60°C Bacillus cereus
1.4.3.19 0.2165
-
 glyphosate mutant enzyme G51R/G60S/D121G/S122P/H164Y/M267T, at pH 8.5 and 60°C Bacillus cereus
1.4.3.19 0.217
-
 glycine mutant enzyme BG51R/G60S/I198V/E357G, at pH 8.5 and 60°C Bacillus cereus
1.4.3.19 0.22
-
 glyphosate mutant enzyme G51R/G60S/K133R/S210P/R250G/V262I, at pH 8.5 and 60°C Bacillus cereus
1.4.3.19 0.513
-
 glyphosate mutant enzyme BG51R/G60S/I198V/E357G, at pH 8.5 and 60°C Bacillus cereus
1.4.3.19 64
-
 glyphosate mutant enzyme G51R/D60G/K133R/V262I, at pH 8.5 and 60°C Bacillus cereus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.19 8.5
-
-
 Bacillus cereus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.3.19 6 11 the enzyme remains stable between pH 6.0 and 11.0 after 6 h incubation Bacillus cereus