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Literature summary extracted from

  • Park, S.; Kim, E.; Noh, W.; Park, H.; Oh, Y.; Lee, S.; Song, B.; Jegal, J.; Lee, S.
    Metabolic engineering of Escherichia coli for the production of 5-aminovalerate and glutarate as C5 platform chemicals (2013), Metab. Eng., 16, 42-47 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.20 gene davD, recombinant expression in Escherichia coli strain W3110, coexpression with Pseudomonas putida gene gabT, encoding 5-aminovalerate aminotransferase, and davA and davB genes encoding delta-aminovaleramidase and lysine 2-monooxygenase, respectively. The recombinant Escherichia coli WL3110 strain expressingthe davAB and gabTD genes is cultured in a medium containing D-glucose, L-lysine, and 2-oxoglutarate, and produces glutarate Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.20 additional information metabolic engineering strategies for the production of 5-aminovalerate from L-lysine in vivo synthesized from glucose in Escherichia coli by amplifying metabolic fluxes and activities, e.g. enzyme glutarate semialdehyde dehydrogenase, and by removing repressions and feedback inhibitions involved in L-lysine metabolism, oevrview Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.20 glutarate semialdehyde + NAD+ + H2O Pseudomonas putida
-
 glutarate + NADH + 2 H+
-
 ?
1.2.1.20 glutarate semialdehyde + NAD+ + H2O Pseudomonas putida ATCC 47054
-
 glutarate + NADH + 2 H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.20 Pseudomonas putida Q88RC0
-
-
1.2.1.20 Pseudomonas putida ATCC 47054 Q88RC0
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.20 glutarate semialdehyde + NAD+ + H2O
-
 Pseudomonas putida glutarate + NADH + 2 H+
-
 ?
1.2.1.20 glutarate semialdehyde + NAD+ + H2O
-
 Pseudomonas putida ATCC 47054 glutarate + NADH + 2 H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.20 davD
-
 Pseudomonas putida
1.2.1.20 glutarate semialdehyde dehydrogenase
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.20 NAD+
-
 Pseudomonas putida

General Information

EC Number General Information Comment Organism
1.2.1.20 metabolism the enzyme is involved in the metabolic pathways for the production of 5-aminovalerate and glutarate from L-lysine Pseudomonas putida