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Literature summary extracted from

  • Borisova, M.; Gisin, J.; Mayer, C.
    The N-acetylmuramic acid 6-phosphate phosphatase MupP completes the Pseudomonas peptidoglycan recycling pathway leading to intrinsic fosfomycin resistance (2017), mBio, 8, e00092 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.105 expression in Escherichia coli Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.105 0.31
-
 N-acetyl-D-muramate 6-phosphate pH 7.6, 22°C Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.105 Mg2+ very low activity in absence of Mg2+, 16fold increase in activity in presence of 10 mM Mg2+ Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.105 Pseudomonas putida Q88M11
-
-
3.1.3.105 Pseudomonas putida DSM 6125 Q88M11
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.105 additional information no substrates: N-acetyl-D-muramate 1-phosphate, N-acetyl-D-glucosamine 6-phosphate, N-acetyl-D-glucosamine 1-phosphate, N-acetyl-D-glucosamine, D-glucose, D-glucosamine 6-phosphate, glucose 6-phosphate Pseudomonas putida ?
-
 ?
3.1.3.105 additional information no substrates: N-acetyl-D-muramate 1-phosphate, N-acetyl-D-glucosamine 6-phosphate, N-acetyl-D-glucosamine 1-phosphate, N-acetyl-D-glucosamine, D-glucose, D-glucosamine 6-phosphate, glucose 6-phosphate Pseudomonas putida DSM 6125 ?
-
 ?
3.1.3.105 N-acetyl-D-muramate 6-phosphate + H2O
-
 Pseudomonas putida N-acetyl-D-muramate + phosphate
-
 ?
3.1.3.105 N-acetyl-D-muramate 6-phosphate + H2O
-
 Pseudomonas putida DSM 6125 N-acetyl-D-muramate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.105 ? x * 25890, calculated, recombinant His-tagged protein Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
3.1.3.105 mupP
-
 Pseudomonas putida
3.1.3.105 N-acetylmuramic acid 6-phosphate phosphatase
-
 Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.105 45
-
-
 Pseudomonas putida

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.105 22
-
 40 min, 10% loss of activity Pseudomonas putida
3.1.3.105 37
-
 40 min, 70% loss of activity Pseudomonas putida
3.1.3.105 45
-
 40 min, complete inactivation Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.105 0.88
-
 N-acetyl-D-muramate 6-phosphate pH 7.6, 22°C Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.105 7.6
-
 assay at Pseudomonas putida

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.105 6 9
-
 Pseudomonas putida

General Information

EC Number General Information Comment Organism
3.1.3.105 physiological function enzyme is part of the anabolic recycling pathway of Pseudomonas species and specifically converts MurNAc 6-phosphate to MurNAc. A MupP mutant is highly susceptible to fosfomycin, accumulates large amounts of MurNAc 6-phosphate, and shows lower levels of UDP-MurNAc than wild-type cells Pseudomonas putida

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.3.105 2.84
-
 N-acetyl-D-muramate 6-phosphate pH 7.6, 22°C Pseudomonas putida