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Literature summary extracted from

  • Simon, O.; Klebensberger, J.; Muekschel, B.; Klaiber, I.; Graf, N.; Altenbuchner, J.; Huber, A.; Hauer, B.; Pfannstiel, J.
    Analysis of the molecular response of Pseudomonas putida KT2440 to the next-generation biofuel n-butanol (2015), J. Proteomics, 122, 11-25 .
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.1.2.8 additional information generation of a single deletion mutant strain of the gene coding for PedE resulting in strain GN104, and of a double mutant with deletion of both genes PedE and PedH, strain GN127 Pseudomonas putida
1.1.2.8 additional information generation of a single deletion mutant strain of the gene coding for PedH resulting in strain GN116, and of a double mutant with deletion of both genes PedE and PedH, strain GN127 Pseudomonas putida

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.2.8 periplasm
-
 Pseudomonas putida
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.2.8 butanol + 2 cytochrome c Pseudomonas putida
-
 butyraldehyde + 2 reduced cytochrome c
-
 r
1.1.2.8 butanol + 2 cytochrome c Pseudomonas putida KT 2240
-
 butyraldehyde + 2 reduced cytochrome c
-
 r
1.1.2.8 ethanol + 2 cytochrome c Pseudomonas putida
-
 acetaldehyde + 2 reduced cytochrome c
-
 r
1.1.2.8 ethanol + 2 cytochrome c Pseudomonas putida KT 2240
-
 acetaldehyde + 2 reduced cytochrome c
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.2.8 Pseudomonas putida B1N7J0
-
-
1.1.2.8 Pseudomonas putida B1N7J5
-
-
1.1.2.8 Pseudomonas putida KT 2240 B1N7J0
-
-
1.1.2.8 Pseudomonas putida KT 2240 B1N7J5
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.2.8 butanol + 2 cytochrome c
-
 Pseudomonas putida butyraldehyde + 2 reduced cytochrome c
-
 r
1.1.2.8 butanol + 2 cytochrome c
-
 Pseudomonas putida KT 2240 butyraldehyde + 2 reduced cytochrome c
-
 r
1.1.2.8 ethanol + 2 cytochrome c
-
 Pseudomonas putida acetaldehyde + 2 reduced cytochrome c
-
 r
1.1.2.8 ethanol + 2 cytochrome c
-
 Pseudomonas putida KT 2240 acetaldehyde + 2 reduced cytochrome c
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.2.8 ethanol dehydrogenase
-
 Pseudomonas putida
1.1.2.8 PedE
-
 Pseudomonas putida
1.1.2.8 PedH
-
 Pseudomonas putida
1.1.2.8 PP_2674
-
 Pseudomonas putida
1.1.2.8 PP_2679
-
 Pseudomonas putida
1.1.2.8 PQQ-dependent alcohol dehydrogenase
-
 Pseudomonas putida
1.1.2.8 quinoprotein alcohol dehydrogenase
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.2.8 cytochrome c
-
 Pseudomonas putida
1.1.2.8 pyrroloquinoline quinone PQQ, a prosthetic group Pseudomonas putida

General Information

EC Number General Information Comment Organism
1.1.2.8 malfunction single deletions of genes coding for PQQ-dependent alcohol dehydrogenases PedE and PedH have only minor effects on growth rates, indicating that Pseudomonas putida strain KT2440 can use both enzymes in a redundant fashion for the metabolization of butanol. Growth of mutants lacking PedE and PedH on n-butanol is significantly impaired, but not completely inhibited, suggesting that additional alcohol dehydrogenases can at least partially complement their function in strain KT2440 Pseudomonas putida
1.1.2.8 metabolism butanol is oxidized to butyraldehyde by PedE and PedH and then further oxidized to butyric acid by the aldehyde dehydrogenase PedI. Both enzymes, PedE and PedH, are directly involved in butanol oxidation in Pseudomonas putida KT2440 Pseudomonas putida