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Literature summary extracted from

  • Philibert, T.; Rao, Z.; Yang, T.; Zhou, J.; Huang, G.; Irene, K.; Samuel, N.
    Heterologous expression and characterization of a new heme-catalase in Bacillus subtilis 168 (2016), J. Ind. Microbiol. Biotechnol., 43, 729-740 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.21 expression in Bacillus subtilis Bacillus pumilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.11.1.21 molecular modeling of structure reveals highly conserved catalytic residues His57 and Asn130 in the heme cavity and Asp110 in the main channel Bacillus pumilus

General Stability

EC Number General Stability Organism
1.11.1.21 20°C, 20 h, 90% residual activity Bacillus pumilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.21 3-Amino-1,2,4-triazole 5 mM, 35% inhibition Bacillus pumilus
1.11.1.21 3-Amino-4-hydroxybenzoic acid 50 mM, 30% residual activity Bacillus pumilus
1.11.1.21 Co2+ 1 mM, 25% residual activity Bacillus pumilus
1.11.1.21 Cu2+ 1 mM, 30% residual activity Bacillus pumilus
1.11.1.21 hydroxylammonium chloride 0.5 mM, 70% inhibition Bacillus pumilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.21 0.97
-
 peroxyacetic acid pH 4.5, 37°C Bacillus pumilus
1.11.1.21 59.6
-
 H2O2 pH 7.0, 37°C Bacillus pumilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.11.1.21 Fe2+ 1 mM, 110% of initial activity Bacillus pumilus

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.21 Bacillus pumilus A0A143G976
-
-
1.11.1.21 Bacillus pumilus ML 413 A0A143G976
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.21 55784
-
 substrate H2O2, pH 7.0, 37°C Bacillus pumilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.21 2 H2O2
-
 Bacillus pumilus 2 H2O + O2
-
 ?
1.11.1.21 2 H2O2
-
 Bacillus pumilus ML 413 2 H2O + O2
-
 ?
1.11.1.21 peroxyacetic acid + 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
-
 Bacillus pumilus ?
-
 ?
1.11.1.21 peroxyacetic acid + 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
-
 Bacillus pumilus ML 413 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.21 katX2
-
 Bacillus pumilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.21 40
-
-
 Bacillus pumilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.21 60
-
 rapid decrease in stability Bacillus pumilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.21 19.42
-
 peroxyacetic acid pH 4.5, 37°C Bacillus pumilus
1.11.1.21 322000
-
 H2O2 pH 7.0, 37°C Bacillus pumilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.21 7
-
-
 Bacillus pumilus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.11.1.21 6 11
-
 Bacillus pumilus
1.11.1.21 9
-
 14 h, 80% residual activity Bacillus pumilus

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.21 heme
-
 Bacillus pumilus

General Information

EC Number General Information Comment Organism
1.11.1.21 physiological function expression in Bacillus subtilis improves its resistance to oxidative stress Bacillus pumilus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.11.1.21 20.02
-
 peroxyacetic acid pH 4.5, 37°C Bacillus pumilus
1.11.1.21 5410
-
 H2O2 pH 7.0, 37°C Bacillus pumilus