Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Wang, S.S.; Ning, Y.J.; Wang, S.N.; Zhang, J.; Zhang, G.Q.; Chen, Q.J.
    Purification, characterization, and cloning of an extracellular laccase with potent dye decolorizing ability from white rot fungus Cerrena unicolor GSM-01 (2017), Int. J. Biol. Macromol., 95, 920-927 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.10.3.2 degradation laccase can be efficiently used to decolorize synthetic dye and help in waste water treatment. The enzyme is effective in the decolorization of bromothymol blue, evans blue, methyl orange, and malachite green with decolorizationefficiencies of 50%-85% Cerrena unicolor
1.10.3.2 environmental protection laccase can be efficiently used to decolorize synthetic dye and help in waste water treatment. The enzyme is effective in the decolorization of bromothymol blue, evans blue, methyl orange, and malachite green with decolorizationefficiencies of 50%-85% Cerrena unicolor

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.10.3.2
-
 Cerrena unicolor

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 Ca2+ 1.25 mM, 8% inhibition. 10 mM, 17% inhibition Cerrena unicolor
1.10.3.2 Cd2+ 1.25 mM, 6% inhibition. 10 mM, 20% inhibition Cerrena unicolor
1.10.3.2 Co2+ 1.25 mM, 3% inhibition. 10 mM, 17% inhibition Cerrena unicolor
1.10.3.2 Cu2+ 1.25 mM, 9% inhibition. 10 mM, 19% inhibition Cerrena unicolor
1.10.3.2 EDTA 10 mM, 10% inhibition Cerrena unicolor
1.10.3.2 Fe2+ 1.25 mM, 31% inhibition. 10 mM, 61% inhibition Cerrena unicolor
1.10.3.2 Fe3+ 1.25 mM, 46% inhibition. 10 mM, 93% inhibition Cerrena unicolor
1.10.3.2 K+ 10 mM, 4% inhibition Cerrena unicolor
1.10.3.2 Mg2+ 10 mM, 8% inhibition Cerrena unicolor
1.10.3.2 Na+ 1.25 mM, 4% inhibition. 10 mM, 20% inhibition Cerrena unicolor
1.10.3.2 Zn2+ 10 mM, 13% inhibition Cerrena unicolor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.303
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 3.0, 25°C Cerrena unicolor

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.10.3.2 extracellular
-
 Cerrena unicolor
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 K+ 1.25 mM, 1.08fold activation Cerrena unicolor
1.10.3.2 Mn2+ 1.25-10 mM, slightly enhance the laccase activity of 3.8–10.5% Cerrena unicolor

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 63200
-
-
 Cerrena unicolor

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Cerrena unicolor
-
-
-
1.10.3.2 Cerrena unicolor GSM-01
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2
-
 Cerrena unicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Cerrena unicolor ?
-
 ?
1.10.3.2 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Cerrena unicolor GSM-01 ?
-
 ?
1.10.3.2 catechol + O2 1.8% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Cerrena unicolor ?
-
 ?
1.10.3.2 catechol + O2 1.8% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Cerrena unicolor GSM-01 ?
-
 ?
1.10.3.2 guaiacol + O2 2.3% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Cerrena unicolor ?
-
 ?
1.10.3.2 guaiacol + O2 2.3% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Cerrena unicolor GSM-01 ?
-
 ?
1.10.3.2 o-toluidine + O2 15.7% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Cerrena unicolor ?
-
 ?
1.10.3.2 o-toluidine + O2 15.7% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Cerrena unicolor GSM-01 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 monomer 1 * 63200,SDS-PAGE Cerrena unicolor

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 45
-
 substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Cerrena unicolor

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.10.3.2 30 60 30°C: about 55% of maximal activity, 65°C: about 55% of maximal activity, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Cerrena unicolor

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.3.2 286.5
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 3.0, 25°C Cerrena unicolor

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 2.6
-
 substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Cerrena unicolor

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.10.3.2 2.2 4.3 pH 2.2: about 90% of maximal activity, pH 4.3: about 60% of maximal activity, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Cerrena unicolor

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.10.3.2 946
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 3.0, 25°C Cerrena unicolor