EC Number | Application | Comment | Organism |
---|---|---|---|
3.1.1.3 | synthesis | synthesis of eugenyl benzoate by esterification of eugenol and benzoic acid catalyzed by the chitosan-chitin nanowhiskers supported lipase. Under optimum conditions, a maximum conversion yield of 66% at 50°C in 5 h using 3 mg/ml of lipase, and a substrate molar ratio (eugenol: benzoic acid) of 3:1 is obtained. The lipase is reusable up to 8 esterification cycles and shows higher thermal stability than free enzyme | Rhizomucor miehei |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.3 | eugenol | substrate inhibition | Rhizomucor miehei |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.3 | 34.04 | - |
benzoic acid | chitosan-chitin nanowhiskers supported lipase, pH not specified in the publication, 50°C | Rhizomucor miehei | |
3.1.1.3 | 138.28 | - |
eugenol | chitosan-chitin nanowhiskers supported lipase, pH not specified in the publication, 50°C | Rhizomucor miehei |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.3 | Rhizomucor miehei | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.1.3 | commercial preparation | - |
Rhizomucor miehei | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.3 | eugenol + benzoic acid | - |
Rhizomucor miehei | eugenyl benzoate | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.3 | 0.67 | - |
eugenol | chitosan-chitin nanowhiskers supported lipase, pH not specified in the publication, 50°C | Rhizomucor miehei |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.3 | 438.6 | - |
eugenol | chitosan-chitin nanowhiskers supported lipase, pH not specified in the publication, 50°C | Rhizomucor miehei |