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Literature summary extracted from

  • Liu, Y.N.; Zhang, F.; Li, J.; Li, D.B.; Liu, D.F.; Li, W.W.; Yu, H.Q.
    Exclusive extracellular bioreduction of methyl orange by azo reductase-free Geobacter sulfurreducens (2017), Environ. Sci. Technol., 51, 8616-8623 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.7.1.6 environmental protection Geobacter sulfurreducens useful for the decontamination of environments polluted with azo dyes. The contribution of extracellular respiration to pollutants reduction will broaden the environmental applications Geobacter sulfurreducens

Protein Variants

EC Number Protein Variants Comment Organism
1.7.1.6 additional information deletion of genes omcB, omcC, and omcE encoding outer membrane proteins of Geobacter sulfurrescens modestly inhibit the decolorization activity by 16%, 42%, and 35%, respectively Geobacter sulfurreducens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.6 additional information
-
 additional information Michaelis-Menten kinetics Geobacter sulfurreducens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.7.1.6 extracellular
-
 Geobacter sulfurreducens
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.1.6 4-(dimethylamino)azobenzene + NADPH + H+ Geobacter sulfurreducens
-
 N,N-dimethyl-1,4-phenylenediamine + aniline + NADP+
-
 ?
1.7.1.6 4-(dimethylamino)azobenzene + NADPH + H+ Geobacter sulfurreducens PCA
-
 N,N-dimethyl-1,4-phenylenediamine + aniline + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.6 Geobacter sulfurreducens
-
-
-
1.7.1.6 Geobacter sulfurreducens PCA
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.6 4-(dimethylamino)azobenzene + NADPH + H+
-
 Geobacter sulfurreducens N,N-dimethyl-1,4-phenylenediamine + aniline + NADP+
-
 ?
1.7.1.6 4-(dimethylamino)azobenzene + NADPH + H+
-
 Geobacter sulfurreducens PCA N,N-dimethyl-1,4-phenylenediamine + aniline + NADP+
-
 ?
1.7.1.6 4-([4-(dimethylamino)phenyl]diazenyl)benzene-1-sulfonate + NADPH + H+ i.e. methyl orange Geobacter sulfurreducens ? + NADP+
-
 ?
1.7.1.6 4-([4-(dimethylamino)phenyl]diazenyl)benzene-1-sulfonate + NADPH + H+ i.e. methyl orange Geobacter sulfurreducens PCA ? + NADP+
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.6 NADPH
-
 Geobacter sulfurreducens

General Information

EC Number General Information Comment Organism
1.7.1.6 metabolism essential role of exclusively extracellular respiration in azo dye decolorization. Electrons from acetate oxidization by this strain are transferred by the respiratory chain to methyl orange. As Geobacter sulfurreducens strain PCA does not express known azo reductases, extracellular electron transfer is an alternative way to decolor methyl orange. Outermembrane cytochromes are reported to play a prominent role in electron acceptor in strain PCS. The outermembrane proteins, OmcB, OmcC, and OmcE, are identified as the key proteins in extracellular reduction by the electrochemically active bacterium Geobacter sulfurreducens
1.7.1.6 additional information deletion of genes omcB, omcC, and omcE encoding outer membrane proteins of Geobacter sulfurrescens modestly inhibit the decolorization activity by 16%, 42%, and 35%, respectively Geobacter sulfurreducens
1.7.1.6 physiological function the enzyme catalyzes extracellular bioreduction of methyl orange. Geobacter sulfurreducens strain PCA has a very high decolorization efficiency among the methyl orange reducing bacteria Geobacter sulfurreducens