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Literature summary extracted from

  • Lan, E.; Ro, S.; Liao, J.
    Oxygen-tolerant coenzyme A-acylating aldehyde dehydrogenase facilitates efficient photosynthetic n-butanol biosynthesis in cyanobacteria (2013), Energy Environ. Sci., 6, 2672-2681 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.10 gene pduP, recombinant expression in Escherichia coli strain JCL166, strain JCL166 cannot grow anaerobically unless complemented by an exogenous fermentation pathway such as n-butanol biosynthesis. Recombinant coexpression of PduP with the enzymes of the n-butanol synthesis pathway in Synechococcus elongatus strain PCC 7942 results in autotrophic n-butanol production Aeromonas hydrophila
1.2.1.42 gene pduP, recombinant expression in Escherichia coli strain JCL166, strain JCL166 cannot grow anaerobically unless complemented by an exogenous fermentation pathway such as n-butanol biosynthesis. Recombinant coexpression of PduP with the enzymes of the n-butanol synthesis pathway in Synechococcus elongatus strain PCC 7942 does not result in autotrophic n-butanol production, the enzyme from Porphyromonas gingivalis cannot enable anaerobic growth rescue Porphyromonas gingivalis
1.2.1.57 gene bldh, recombinant expression in Escherichia coli strain JCL166, strain JCL166 cannot grow anaerobically unless complemented by an exogenous fermentation pathway such as n-butanol biosynthesis Synechococcus elongatus
1.2.1.57 gene bldh, recombinant expression in Escherichia coli strain JCL166, strain JCL166 cannot grow anaerobically unless complemented by an exogenous fermentation pathway such as n-butanol biosynthesis. Recombinant coexpression of PduP with the enzymes of the n-butanol synthesis pathway in Synechococcus elongatus strain PCC 7942 results in autotrophic n-butanol production Clostridium saccharoperbutylacetonicum
1.2.1.57 gene pduP, recombinant expression in Escherichia coli strain JCL166, strain JCL166 cannot grow anaerobically unless complemented by an exogenous fermentation pathway such as n-butanol biosynthesis. Recombinant coexpression of PduP with the enzymes of the n-butanol synthesis pathway in Synechococcus elongatus strain PCC 7942 results in autotrophic n-butanol production. PduP from Lactobacillus brevis produces more n-butanol than ethanol Levilactobacillus brevis
1.2.1.87 gene pduP, recombinant expression in Escherichia coli strain JCL166, strain JCL166 cannot grow anaerobically unless complemented by an exogenous fermentation pathway such as n-butanol biosynthesis. Recombinant coexpression of PduP with the enzymes of the n-butanol synthesis pathway in Synechococcus elongatus strain PCC 7942 results in autotrophic n-butanol production. PduP from Klebsiella pneumoniae produces more n-butanol than ethanol Klebsiella pneumoniae
1.2.1.87 gene pduP, recombinant expression in Escherichia coli strain JCL166, strain JCL166 cannot grow anaerobically unless complemented by an exogenous fermentation pathway such as n-butanol biosynthesis. Recombinant coexpression of PduP with the enzymes of the n-butanol synthesis pathway in Synechococcus elongatus strain PCC 7942 results in autotrophic n-butanol production. PduP from Listeria monocytogenes produces more ethanol than n-butanol Listeria monocytogenes
1.2.1.87 gene pduP, recombinant expression in Escherichia coli strain JCL166, strain JCL166 cannot grow anaerobically unless complemented by an exogenous fermentation pathway such as n-butanol biosynthesis. Recombinant coexpression of PduP with the enzymes of the n-butanol synthesis pathway in Synechococcus elongatus strain PCC 7942 results in autotrophic n-butanol production. PduP from Salmonella enterica produces more ethanol than n-butanol Salmonella enterica

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.57 additional information design of a coenzyme A (CoA) dependent n-butanol biosynthesis pathway tailored to the metabolic physiology of the cyanobacterium Synechococcus elongatus PCC 7942 by incorporating an ATP driving force and a kinetically irreversible trap. Oxygen-sensitive CoA-acylating butyraldehyde dehydrogenase (Bldh) is exchanged for the oxygen-tolerant PduP from Salmonella enterica. Replacing Bldh with PduP in the n-butanol synthesis pathway results in n-butanol production to a cumulative titer of 404 mg/l with peak productivity of 51 mg/l per day, exceeding the base strain by 20fold. Anaerobic growth rescue of Escherichia coli strain JCL166 by overexpression of the Clostridium butanol pathway with different aldehyde dehydrogenases PduP Synechococcus elongatus
1.2.1.87 additional information design of a coenzyme A (CoA) dependent n-butanol biosynthesis pathway tailored to the metabolic physiology of the cyanobacterium Synechococcus elongatus PCC 7942 by incorporating an ATP driving force and a kinetically irreversible trap. Oxygen-sensitive CoA-acylating butyraldehyde dehydrogenase (Bldh) is exchanged for the oxygen-tolerant PduP from Salmonella enterica. Replacing Bldh with PduP in the n-butanol synthesis pathway results in n-butanol production to a cumulative titer of 404 mg/l with peak productivity of 51 mg/l per day, exceeding the base strain by 20fold. Anaerobic growth rescue of Escherichia coli strain JCL166 by overexpression of the Clostridium butanol pathway with different aldehyde dehydrogenases PduP Salmonella enterica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.10 additional information
-
 additional information no Michaelis-Menten behaviour Aeromonas hydrophila
1.2.1.42 0.076
-
 acetyl-CoA recombinant enzyme, pH 7.15, 30°C Porphyromonas gingivalis
1.2.1.42 0.256
-
 butanoyl-CoA recombinant enzyme, pH 7.15, 30°C Porphyromonas gingivalis
1.2.1.57 additional information
-
 additional information no Michaelis-Menten behaviour Clostridium saccharoperbutylacetonicum
1.2.1.57 0.076
-
 acetyl-CoA recombinant enzyme, pH 7.15, 30°C Levilactobacillus brevis
1.2.1.57 0.534
-
 butanoyl-CoA recombinant enzyme, pH 7.15, 30°C Levilactobacillus brevis
1.2.1.87 additional information
-
 additional information Michaelis-Menten kientics Listeria monocytogenes
1.2.1.87 0.056
-
 butanoyl-CoA recombinant enzyme, pH 7.15, 30°C Klebsiella pneumoniae
1.2.1.87 0.0717
-
 butanoyl-CoA recombinant enzyme, pH 7.15, 30°C Listeria monocytogenes
1.2.1.87 0.087
-
 butanoyl-CoA recombinant enzyme, pH 7.15, 30°C Salmonella enterica
1.2.1.87 0.0926
-
 acetyl-CoA recombinant enzyme, pH 7.15, 30°C Listeria monocytogenes
1.2.1.87 0.181
-
 acetyl-CoA recombinant enzyme, pH 7.15, 30°C Klebsiella pneumoniae
1.2.1.87 0.342
-
 acetyl-CoA recombinant enzyme, pH 7.15, 30°C Salmonella enterica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.10 acetyl-CoA + NADH + H+ Aeromonas hydrophila best substrate acetaldehyde + CoA + NAD+
-
 r
1.2.1.10 butanoyl-CoA + NADH + H+ Aeromonas hydrophila
-
 butanal + CoA + NAD+
-
 r
1.2.1.10 propanoyl-CoA + NADH + H+ Aeromonas hydrophila
-
 propanal + CoA + NAD+
-
 r
1.2.1.42 acetyl-CoA + NADH + H+ Porphyromonas gingivalis
-
 acetaldehyde + CoA + NAD+
-
 r
1.2.1.42 butanoyl-CoA + NADH + H+ Porphyromonas gingivalis
-
 butanal + CoA + NAD+
-
 r
1.2.1.57 acetyl-CoA + NADH + H+ Levilactobacillus brevis
-
 acetaldehyde + CoA + NAD+
-
 r
1.2.1.57 acetyl-CoA + NADH + H+ Clostridium saccharoperbutylacetonicum low activity acetaldehyde + CoA + NAD+
-
 r
1.2.1.57 butanoyl-CoA + NADH + H+ Levilactobacillus brevis
-
 butanal + CoA + NAD+
-
 r
1.2.1.57 butanoyl-CoA + NADH + H+ Synechococcus elongatus
-
 butanal + CoA + NAD+
-
 ?
1.2.1.57 butanoyl-CoA + NADH + H+ Clostridium saccharoperbutylacetonicum best substrate butanal + CoA + NAD+
-
 r
1.2.1.57 butanoyl-CoA + NADH + H+ Synechococcus elongatus PCC 7942
-
 butanal + CoA + NAD+
-
 ?
1.2.1.87 acetyl-CoA + NADH + H+ Klebsiella pneumoniae
-
 acetaldehyde + CoA + NAD+
-
 r
1.2.1.87 acetyl-CoA + NADH + H+ Salmonella enterica
-
 acetaldehyde + CoA + NAD+
-
 r
1.2.1.87 acetyl-CoA + NADH + H+ Listeria monocytogenes low activity acetaldehyde + CoA + NAD+
-
 r
1.2.1.87 acetyl-CoA + NADH + H+ Listeria monocytogenes M7 low activity acetaldehyde + CoA + NAD+
-
 r
1.2.1.87 butanoyl-CoA + NADH + H+ Salmonella enterica
-
 butanal + CoA + NAD+
-
 r
1.2.1.87 butanoyl-CoA + NADH + H+ Listeria monocytogenes
-
 butanal + CoA + NAD+
-
 r
1.2.1.87 butanoyl-CoA + NADH + H+ Klebsiella pneumoniae high activity butanal + CoA + NAD+
-
 r
1.2.1.87 butanoyl-CoA + NADH + H+ Listeria monocytogenes M7
-
 butanal + CoA + NAD+
-
 r
1.2.1.87 propanoyl-CoA + NADH + H+ Klebsiella pneumoniae best substrate propanal + CoA + NAD+
-
 r
1.2.1.87 propanoyl-CoA + NADH + H+ Salmonella enterica best substrate propanal + CoA + NAD+
-
 r
1.2.1.87 propanoyl-CoA + NADH + H+ Listeria monocytogenes best substrate propanal + CoA + NAD+
-
 r
1.2.1.87 propanoyl-CoA + NADH + H+ Listeria monocytogenes M7 best substrate propanal + CoA + NAD+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.10 Aeromonas hydrophila
-
-
-
1.2.1.42 Porphyromonas gingivalis
-
-
-
1.2.1.57 Clostridium saccharoperbutylacetonicum
-
-
-
1.2.1.57 Levilactobacillus brevis
-
-
-
1.2.1.57 Synechococcus elongatus
-
-
-
1.2.1.57 Synechococcus elongatus PCC 7942
-
-
-
1.2.1.87 Klebsiella pneumoniae
-
-
-
1.2.1.87 Listeria monocytogenes A0A0E0UUX4
-
-
1.2.1.87 Listeria monocytogenes M7 A0A0E0UUX4
-
-
1.2.1.87 Salmonella enterica
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.2.1.10 the enzyme is oxygen-tolerant Aeromonas hydrophila
1.2.1.42 the enzyme is oxygen-tolerant Porphyromonas gingivalis
1.2.1.57 oxygen sensitivity of CoA-acylating aldehyde dehydrogenase Synechococcus elongatus
1.2.1.57 the enzyme is oxygen-tolerant Levilactobacillus brevis
1.2.1.57 the enzyme is oxygen-tolerant Clostridium saccharoperbutylacetonicum
1.2.1.87 the enzyme is oxygen-tolerant Klebsiella pneumoniae
1.2.1.87 the enzyme is oxygen-tolerant Salmonella enterica
1.2.1.87 the enzyme is oxygen-tolerant Listeria monocytogenes

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.10 1.7
-
 with butanoyl-CoA, pH 7.15, 30°C Aeromonas hydrophila
1.2.1.42 2.2
-
 about, with butanoyl-CoA, pH 7.15, 30°C Porphyromonas gingivalis
1.2.1.57 0.49
-
 with butanoyl-CoA, pH 7.15, 30°C Clostridium saccharoperbutylacetonicum
1.2.1.57 2.5
-
 with butanoyl-CoA, pH 7.15, 30°C Levilactobacillus brevis
1.2.1.87 8.9
-
 with butanoyl-CoA, pH 7.15, 30°C Klebsiella pneumoniae
1.2.1.87 17
-
 with butanoyl-CoA, pH 7.15, 30°C Listeria monocytogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.10 acetyl-CoA + NADH + H+ best substrate Aeromonas hydrophila acetaldehyde + CoA + NAD+
-
 r
1.2.1.10 butanoyl-CoA + NADH + H+
-
 Aeromonas hydrophila butanal + CoA + NAD+
-
 ?
1.2.1.10 butanoyl-CoA + NADH + H+
-
 Aeromonas hydrophila butanal + CoA + NAD+
-
 r
1.2.1.10 additional information substrate chain length specificity of the enzyme is C2-C4, overview Aeromonas hydrophila ?
-
 ?
1.2.1.10 propanoyl-CoA + NADH + H+
-
 Aeromonas hydrophila propanal + CoA + NAD+
-
 r
1.2.1.42 acetyl-CoA + NADH + H+
-
 Porphyromonas gingivalis acetaldehyde + CoA + NAD+
-
 r
1.2.1.42 butanoyl-CoA + NADH + H+
-
 Porphyromonas gingivalis butanal + CoA + NAD+
-
 r
1.2.1.42 additional information substrate chain length specificity of the enzyme is C2-C12, highest activity with C12 substrate, overview. The overall activity of the enzyme is low compared to other PduP enzymes Porphyromonas gingivalis ?
-
 ?
1.2.1.57 acetyl-CoA + NADH + H+
-
 Levilactobacillus brevis acetaldehyde + CoA + NAD+
-
 r
1.2.1.57 acetyl-CoA + NADH + H+ low activity Clostridium saccharoperbutylacetonicum acetaldehyde + CoA + NAD+
-
 r
1.2.1.57 butanoyl-CoA + NADH + H+
-
 Levilactobacillus brevis butanal + CoA + NAD+
-
 r
1.2.1.57 butanoyl-CoA + NADH + H+
-
 Synechococcus elongatus butanal + CoA + NAD+
-
 ?
1.2.1.57 butanoyl-CoA + NADH + H+ best substrate Clostridium saccharoperbutylacetonicum butanal + CoA + NAD+
-
 r
1.2.1.57 butanoyl-CoA + NADH + H+
-
 Synechococcus elongatus PCC 7942 butanal + CoA + NAD+
-
 ?
1.2.1.57 additional information substrate chain length specificity of the enzyme is C2-C12, highest activity with C6 substrate, overview Levilactobacillus brevis ?
-
 ?
1.2.1.87 acetyl-CoA + NADH + H+
-
 Klebsiella pneumoniae acetaldehyde + CoA + NAD+
-
 r
1.2.1.87 acetyl-CoA + NADH + H+
-
 Salmonella enterica acetaldehyde + CoA + NAD+
-
 r
1.2.1.87 acetyl-CoA + NADH + H+ low activity Listeria monocytogenes acetaldehyde + CoA + NAD+
-
 r
1.2.1.87 acetyl-CoA + NADH + H+ preferred substrate Listeria monocytogenes acetaldehyde + CoA + NAD+
-
 r
1.2.1.87 acetyl-CoA + NADH + H+ low activity Listeria monocytogenes M7 acetaldehyde + CoA + NAD+
-
 r
1.2.1.87 acetyl-CoA + NADH + H+ preferred substrate Listeria monocytogenes M7 acetaldehyde + CoA + NAD+
-
 r
1.2.1.87 butanoyl-CoA + NADH + H+
-
 Salmonella enterica butanal + CoA + NAD+
-
 r
1.2.1.87 butanoyl-CoA + NADH + H+
-
 Listeria monocytogenes butanal + CoA + NAD+
-
 r
1.2.1.87 butanoyl-CoA + NADH + H+ high activity Klebsiella pneumoniae butanal + CoA + NAD+
-
 r
1.2.1.87 butanoyl-CoA + NADH + H+
-
 Listeria monocytogenes M7 butanal + CoA + NAD+
-
 r
1.2.1.87 additional information substrate chain length specificity of the enzyme is C2-C12, overview Klebsiella pneumoniae ?
-
 ?
1.2.1.87 additional information substrate chain length specificity of the enzyme is C2-C12, overview Salmonella enterica ?
-
 ?
1.2.1.87 additional information substrate chain length specificity of the enzyme is C2-C12, overview Listeria monocytogenes ?
-
 ?
1.2.1.87 additional information substrate chain length specificity of the enzyme is C2-C12, overview Listeria monocytogenes M7 ?
-
 ?
1.2.1.87 propanoyl-CoA + NADH + H+ best substrate Klebsiella pneumoniae propanal + CoA + NAD+
-
 r
1.2.1.87 propanoyl-CoA + NADH + H+ best substrate Salmonella enterica propanal + CoA + NAD+
-
 r
1.2.1.87 propanoyl-CoA + NADH + H+ best substrate Listeria monocytogenes propanal + CoA + NAD+
-
 r
1.2.1.87 propanoyl-CoA + NADH + H+ best substrate Listeria monocytogenes M7 propanal + CoA + NAD+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.2.1.10 CoA-acylating aldehyde dehydrogenase
-
 Aeromonas hydrophila
1.2.1.10 coenzyme A-acylating aldehyde dehydrogenase
-
 Aeromonas hydrophila
1.2.1.10 PduP
-
 Aeromonas hydrophila
1.2.1.42 CoA-acylating aldehyde dehydrogenase
-
 Porphyromonas gingivalis
1.2.1.42 coenzyme A-acylating aldehyde dehydrogenase
-
 Porphyromonas gingivalis
1.2.1.42 PduP
-
 Porphyromonas gingivalis
1.2.1.57 Bldh
-
 Synechococcus elongatus
1.2.1.57 Bldh
-
 Clostridium saccharoperbutylacetonicum
1.2.1.57 CoA-acylating aldehyde dehydrogenase
-
 Levilactobacillus brevis
1.2.1.57 CoA-acylating aldehyde dehydrogenase
-
 Synechococcus elongatus
1.2.1.57 CoA-acylating aldehyde dehydrogenase
-
 Clostridium saccharoperbutylacetonicum
1.2.1.57 CoA-acylating butyraldehyde dehydrogenase
-
 Synechococcus elongatus
1.2.1.57 coenzyme A-acylating aldehyde dehydrogenase
-
 Levilactobacillus brevis
1.2.1.57 coenzyme A-acylating aldehyde dehydrogenase
-
 Synechococcus elongatus
1.2.1.57 coenzyme A-acylating aldehyde dehydrogenase
-
 Clostridium saccharoperbutylacetonicum
1.2.1.57 PduP
-
 Levilactobacillus brevis
1.2.1.87 CoA-acylating aldehyde dehydrogenase
-
 Klebsiella pneumoniae
1.2.1.87 CoA-acylating aldehyde dehydrogenase
-
 Salmonella enterica
1.2.1.87 CoA-acylating aldehyde dehydrogenase
-
 Listeria monocytogenes
1.2.1.87 coenzyme A-acylating aldehyde dehydrogenase
-
 Klebsiella pneumoniae
1.2.1.87 coenzyme A-acylating aldehyde dehydrogenase
-
 Salmonella enterica
1.2.1.87 coenzyme A-acylating aldehyde dehydrogenase
-
 Listeria monocytogenes
1.2.1.87 More cf. EC 1.2.1.57 Salmonella enterica
1.2.1.87 PduP
-
 Klebsiella pneumoniae
1.2.1.87 PduP
-
 Salmonella enterica
1.2.1.87 PduP
-
 Listeria monocytogenes

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.10 30
-
 assay at Aeromonas hydrophila
1.2.1.42 30
-
 assay at Porphyromonas gingivalis
1.2.1.57 30
-
 assay at Levilactobacillus brevis
1.2.1.57 30
-
 assay at Clostridium saccharoperbutylacetonicum
1.2.1.87 30
-
 assay at Klebsiella pneumoniae
1.2.1.87 30
-
 assay at Salmonella enterica
1.2.1.87 30
-
 assay at Listeria monocytogenes

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.42 0.08
-
 acetyl-CoA recombinant enzyme, pH 7.15, 30°C Porphyromonas gingivalis
1.2.1.42 0.19
-
 butanoyl-CoA recombinant enzyme, pH 7.15, 30°C Porphyromonas gingivalis
1.2.1.57 0.26
-
 acetyl-CoA recombinant enzyme, pH 7.15, 30°C Levilactobacillus brevis
1.2.1.57 3.37
-
 butanoyl-CoA recombinant enzyme, pH 7.15, 30°C Levilactobacillus brevis
1.2.1.87 4.63
-
 acetyl-CoA recombinant enzyme, pH 7.15, 30°C Klebsiella pneumoniae
1.2.1.87 7.02
-
 butanoyl-CoA recombinant enzyme, pH 7.15, 30°C Klebsiella pneumoniae
1.2.1.87 11.33
-
 acetyl-CoA recombinant enzyme, pH 7.15, 30°C Listeria monocytogenes
1.2.1.87 11.99
-
 butanoyl-CoA recombinant enzyme, pH 7.15, 30°C Listeria monocytogenes
1.2.1.87 14.81
-
 acetyl-CoA recombinant enzyme, pH 7.15, 30°C Salmonella enterica
1.2.1.87 25.38
-
 butanoyl-CoA recombinant enzyme, pH 7.15, 30°C Salmonella enterica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.10 7.15
-
 assay at Aeromonas hydrophila
1.2.1.42 7.15
-
 assay at Porphyromonas gingivalis
1.2.1.57 7.15
-
 assay at Levilactobacillus brevis
1.2.1.57 7.15
-
 assay at Clostridium saccharoperbutylacetonicum
1.2.1.87 7.15
-
 assay at Klebsiella pneumoniae
1.2.1.87 7.15
-
 assay at Salmonella enterica
1.2.1.87 7.15
-
 assay at Listeria monocytogenes

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.10 NADH
-
 Aeromonas hydrophila
1.2.1.42 NADH
-
 Porphyromonas gingivalis
1.2.1.57 NADH
-
 Levilactobacillus brevis
1.2.1.57 NADH
-
 Synechococcus elongatus
1.2.1.57 NADH
-
 Clostridium saccharoperbutylacetonicum
1.2.1.87 NADH
-
 Klebsiella pneumoniae
1.2.1.87 NADH
-
 Salmonella enterica
1.2.1.87 NADH
-
 Listeria monocytogenes

General Information

EC Number General Information Comment Organism
1.2.1.10 metabolism the oxygen sensitivity of CoA-acylating aldehyde dehydrogenase appears to be a key limiting factor for cyanobacteria to produce alcohols through the CoA-dependent route Aeromonas hydrophila
1.2.1.57 metabolism the oxygen sensitivity of CoA-acylating aldehyde dehydrogenase appears to be a key limiting factor for cyanobacteria to produce alcohols through the CoA-dependent route Levilactobacillus brevis
1.2.1.57 metabolism the oxygen sensitivity of CoA-acylating aldehyde dehydrogenase appears to be a key limiting factor for cyanobacteria to produce alcohols through the CoA-dependent route Synechococcus elongatus
1.2.1.57 metabolism the oxygen sensitivity of CoA-acylating aldehyde dehydrogenase appears to be a key limiting factor for cyanobacteria to produce alcohols through the CoA-dependent route Clostridium saccharoperbutylacetonicum
1.2.1.87 metabolism the oxygen sensitivity of CoA-acylating aldehyde dehydrogenase appears to be a key limiting factor for cyanobacteria to produce alcohols through the CoA-dependent route Salmonella enterica
1.2.1.87 metabolism the oxygen sensitivity of CoA-acylating aldehyde dehydrogenase appears to be a key limiting factor for cyanobacteria to produce alcohols through the CoA-dependent route Listeria monocytogenes

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.2.1.42 0.7
-
 butanoyl-CoA recombinant enzyme, pH 7.15, 30°C Porphyromonas gingivalis
1.2.1.42 1.1
-
 acetyl-CoA recombinant enzyme, pH 7.15, 30°C Porphyromonas gingivalis
1.2.1.57 3
-
 acetyl-CoA recombinant enzyme, pH 7.15, 30°C Levilactobacillus brevis
1.2.1.57 6
-
 butanoyl-CoA recombinant enzyme, pH 7.15, 30°C Levilactobacillus brevis
1.2.1.87 25
-
 acetyl-CoA recombinant enzyme, pH 7.15, 30°C Klebsiella pneumoniae
1.2.1.87 43
-
 acetyl-CoA recombinant enzyme, pH 7.15, 30°C Salmonella enterica
1.2.1.87 122
-
 acetyl-CoA recombinant enzyme, pH 7.15, 30°C Listeria monocytogenes
1.2.1.87 125
-
 butanoyl-CoA recombinant enzyme, pH 7.15, 30°C Klebsiella pneumoniae
1.2.1.87 167
-
 butanoyl-CoA recombinant enzyme, pH 7.15, 30°C Listeria monocytogenes
1.2.1.87 292
-
 butanoyl-CoA recombinant enzyme, pH 7.15, 30°C Salmonella enterica