EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.13.2 | expressed in Escherichia coli BL21 Star (DE3) cells | Pseudomonas aeruginosa |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.13.2 | Y385F | the mutant displays higher activity toward 3,4-dihydroxybenzoic acid than the wild type enzyme | Pseudomonas aeruginosa |
1.14.13.2 | Y385F/T294A | the mutant displays much higher activity toward 3,4-dihydroxybenzoic acid than the wild type enzyme | Pseudomonas aeruginosa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.13.2 | 0.01955 | - |
4-hydroxybenzoate | mutant Y385F, at pH 8.0 and 30°C | Pseudomonas aeruginosa | |
1.14.13.2 | 0.03467 | - |
4-hydroxybenzoate | wild type enzyme, at pH 8.0 and 30°C | Pseudomonas aeruginosa | |
1.14.13.2 | 0.04838 | - |
4-hydroxybenzoate | mutant Y385F/T294A, at pH 8.0 and 30°C | Pseudomonas aeruginosa | |
1.14.13.2 | 0.15702 | - |
3,4-dihydroxybenzoic acid | mutant Y385F/T294A, at pH 8.0 and 30°C | Pseudomonas aeruginosa | |
1.14.13.2 | 0.22802 | - |
3,4-dihydroxybenzoic acid | mutant Y385F, at pH 8.0 and 30°C | Pseudomonas aeruginosa |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.2 | 4-hydroxybenzoate + NADPH + H+ + O2 | Pseudomonas aeruginosa | - |
protocatechuate + NADP+ + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.2 | Pseudomonas aeruginosa | - |
- |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.13.2 | 0.26 | - |
mutant enzyme Y385F, with 4-hydroxybenzoate as substrate, at pH 8.0 and 30°C | Pseudomonas aeruginosa |
1.14.13.2 | 0.45 | - |
mutant enzyme Y385F, with 3,4-dihydroxybenzoic acid as substrate, at pH 8.0 and 30°C | Pseudomonas aeruginosa |
1.14.13.2 | 1.04 | - |
mutant enzyme Y385F/T294A, with 4-hydroxybenzoate as substrate, at pH 8.0 and 30°C | Pseudomonas aeruginosa |
1.14.13.2 | 1.86 | - |
mutant enzyme Y385F/T294A, with 3,4-dihydroxybenzoic acid as substrate, at pH 8.0 and 30°C | Pseudomonas aeruginosa |
1.14.13.2 | 16.85 | - |
wild type enzyme, with 4-hydroxybenzoate as substrate, at pH 8.0 and 30°C | Pseudomonas aeruginosa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.2 | 3,4-dihydroxybenzoic acid + NADPH + H+ + O2 | weak activity | Pseudomonas aeruginosa | gallic acid + NADP+ + H2O | - |
? | |
1.14.13.2 | 4-hydroxybenzoate + NADPH + H+ + O2 | - |
Pseudomonas aeruginosa | protocatechuate + NADP+ + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.13.2 | p-hydroxybenzoate hydroxylase | - |
Pseudomonas aeruginosa |
1.14.13.2 | PobA | - |
Pseudomonas aeruginosa |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.13.2 | 0.2 | - |
4-hydroxybenzoate | mutant Y385F, at pH 8.0 and 30°C | Pseudomonas aeruginosa | |
1.14.13.2 | 0.39 | - |
3,4-dihydroxybenzoic acid | mutant Y385F, at pH 8.0 and 30°C | Pseudomonas aeruginosa | |
1.14.13.2 | 0.9 | - |
4-hydroxybenzoate | mutant Y385F/T294A, at pH 8.0 and 30°C | Pseudomonas aeruginosa | |
1.14.13.2 | 1.69 | - |
3,4-dihydroxybenzoic acid | mutant Y385F/T294A, at pH 8.0 and 30°C | Pseudomonas aeruginosa | |
1.14.13.2 | 14.12 | - |
4-hydroxybenzoate | wild type enzyme, at pH 8.0 and 30°C | Pseudomonas aeruginosa |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.2 | FAD | - |
Pseudomonas aeruginosa | |
1.14.13.2 | NADPH | - |
Pseudomonas aeruginosa |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.13.2 | 2 | - |
3,4-dihydroxybenzoic acid | mutant Y385F, at pH 8.0 and 30°C | Pseudomonas aeruginosa | |
1.14.13.2 | 10 | - |
4-hydroxybenzoate | mutant Y385F, at pH 8.0 and 30°C | Pseudomonas aeruginosa | |
1.14.13.2 | 12 | - |
3,4-dihydroxybenzoic acid | mutant Y385F/T294A, at pH 8.0 and 30°C | Pseudomonas aeruginosa | |
1.14.13.2 | 20 | - |
4-hydroxybenzoate | mutant Y385F/T294A, at pH 8.0 and 30°C | Pseudomonas aeruginosa | |
1.14.13.2 | 410 | - |
4-hydroxybenzoate | wild type enzyme, at pH 8.0 and 30°C | Pseudomonas aeruginosa |