EC Number | Cloned (Comment) | Organism |
---|---|---|
1.5.99.14 | expressed in Escherichia coli | Agrobacterium tumefaciens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.5.99.4 | Fe2+ | the enzyme contains a [2Fe-2S]-center | Agrobacterium tumefaciens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.163 | 4-(6-hydroxypyridin-3-yl)-4-oxobutanoate + 2 NADH + 2 H+ + O2 | Agrobacterium tumefaciens | - |
2,5-dihydroxypyridine + succinate semialdehyde + 2 NAD+ + H2O | - |
? | |
1.14.13.163 | 4-(6-hydroxypyridin-3-yl)-4-oxobutanoate + 2 NADH + 2 H+ + O2 | Agrobacterium tumefaciens S33 | - |
2,5-dihydroxypyridine + succinate semialdehyde + 2 NAD+ + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.99.4 | Agrobacterium tumefaciens | - |
- |
- |
1.5.99.4 | Agrobacterium tumefaciens S33 | - |
- |
- |
1.5.99.14 | Agrobacterium tumefaciens | - |
- |
- |
1.5.99.14 | Agrobacterium tumefaciens S33 | - |
- |
- |
1.14.13.163 | Agrobacterium tumefaciens | W8E0Y0 | - |
- |
1.14.13.163 | Agrobacterium tumefaciens S33 | W8E0Y0 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.99.4 | ammonium sulfate precipitation, phenyl Sepharose column chromatography, DEAE-Sepharose column chromatography, and Superdex 200 gel filtration | Agrobacterium tumefaciens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.99.4 | (S)-nicotine + 2,6-dichlorophenolindophenol + H2O | - |
Agrobacterium tumefaciens | (S)-6-hydroxynicotine + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.5.99.4 | (S)-nicotine + 2,6-dichlorophenolindophenol + H2O | - |
Agrobacterium tumefaciens S33 | (S)-6-hydroxynicotine + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.5.99.14 | 6-hydroxypseudooxynicotine + 2,6-dichlorophenolindophenol + H2O | - |
Agrobacterium tumefaciens | 6-hydroxy-3-succinoylsemialdehyde-pyridine + methylamine + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.5.99.14 | 6-hydroxypseudooxynicotine + 2,6-dichlorophenolindophenol + H2O | - |
Agrobacterium tumefaciens S33 | 6-hydroxy-3-succinoylsemialdehyde-pyridine + methylamine + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.14.13.163 | 4-(6-hydroxypyridin-3-yl)-4-oxobutanoate + 2 NADH + 2 H+ + O2 | - |
Agrobacterium tumefaciens | 2,5-dihydroxypyridine + succinate semialdehyde + 2 NAD+ + H2O | - |
? | |
1.14.13.163 | 4-(6-hydroxypyridin-3-yl)-4-oxobutanoate + 2 NADH + 2 H+ + O2 | - |
Agrobacterium tumefaciens S33 | 2,5-dihydroxypyridine + succinate semialdehyde + 2 NAD+ + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.5.99.4 | heterodimer | 1 * 75000 + 1 * 15000, SDS-PAGE | Agrobacterium tumefaciens |
1.5.99.4 | heterodimer | 1 * 82400 + 1 * 17100, calculated from amino acid sequence | Agrobacterium tumefaciens |
1.5.99.14 | ? | x * 73349, calculated from amino acid sequence | Agrobacterium tumefaciens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.5.99.4 | NdhAB | - |
Agrobacterium tumefaciens |
1.5.99.14 | PNO | - |
Agrobacterium tumefaciens |
1.14.13.163 | 6-hydroxy-3-succinoylpyridine hydroxylase | - |
Agrobacterium tumefaciens |
1.14.13.163 | AWN88_01205 | gene name, UniProt | Agrobacterium tumefaciens |
1.14.13.163 | Hsh | - |
Agrobacterium tumefaciens |
1.14.13.163 | HSP hydroxylase | - |
Agrobacterium tumefaciens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.99.4 | molybdopterin | - |
Agrobacterium tumefaciens | |
1.5.99.4 | [2Fe-2S]-center | - |
Agrobacterium tumefaciens | |
1.5.99.14 | FMN | - |
Agrobacterium tumefaciens | |
1.5.99.14 | [4Fe-4S]-center | - |
Agrobacterium tumefaciens | |
1.14.13.163 | FAD | required for activity | Agrobacterium tumefaciens | |
1.14.13.163 | NADH | dependent on | Agrobacterium tumefaciens |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.13.163 | metabolism | 6-hydroxy-3-succinoylpyridine hydroxylase catalyzes a central step of nicotine degradation. 6-Hydroxy-3-succinoylpyridine (HSP) is a key intermediate connecting the two pathways, pyridine pathway and pyrrolidine pathway, detailed overview | Agrobacterium tumefaciens |
1.14.13.163 | physiological function | the key enzyme HSP hydroxylase is involved in the fused nicotine degradation pathway of the pyridine and pyrrolidine pathways | Agrobacterium tumefaciens |