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Literature summary extracted from

  • Brissos, V.; Tavares, D.; Sousa, A.; Robalo, M.; Martins, L.
    Engineering a bacterial DyP-type peroxidase for enhanced oxidation of lignin-related phenolics at alkaline pH (2017), ACS Catal., 7, 3454-3465 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.19 expressed in Escherichia coli BL21 Star (DE3) cells Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.19 E188K the mutant shows 3-4fold higher activity for 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) in comparison to the wild type enzyme Pseudomonas putida
1.11.1.19 E188K/A142V the mutant shows 2fold higher activity for 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) in comparison to the wild type enzyme Pseudomonas putida
1.11.1.19 E188K/A142V/H125Y the mutant shows a 100fold enhanced catalytic efficiency (kcat/Km) for 2,6-dimethoxyphenol and 2fold higher activity for 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) in comparison to the wild type enzyme Pseudomonas putida
1.11.1.19 E188K/H125R the mutant shows 6fold higher activity for 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) in comparison to the wild type enzyme Pseudomonas putida
1.11.1.19 H125R the mutant shows 3-4fold higher activity for 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) in comparison to the wild type enzyme Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.19 H2O2
-
 Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.19 0.023
-
 2,6-dimethoxyphenol mutant enzyme E188K/H125R, at pH 4.3 and 25°C Pseudomonas putida
1.11.1.19 0.042
-
 2,6-dimethoxyphenol mutant enzyme E188K/A142V, at pH 5.5 and 25°C Pseudomonas putida
1.11.1.19 0.044
-
 H2O2 mutant enzyme E188K/A142V/H125Y, at pH 5.5 and 25°C Pseudomonas putida
1.11.1.19 0.047
-
 H2O2 mutant enzyme A142V, at pH 5.5 and 25°C Pseudomonas putida
1.11.1.19 0.058
-
 2,6-dimethoxyphenol mutant enzyme E188K/A142V/H125Y, at pH 5.5 and 25°C Pseudomonas putida
1.11.1.19 0.06
-
 2,6-dimethoxyphenol mutant enzyme E188K, at pH 4.3 and 25°C Pseudomonas putida
1.11.1.19 0.06
-
 H2O2 wild type enzyme, at pH 4.3 and 25°C Pseudomonas putida
1.11.1.19 0.07
-
 2,6-dimethoxyphenol wild type enzyme, at pH 4.3 and 25°C Pseudomonas putida
1.11.1.19 0.08
-
 2,6-dimethoxyphenol mutant enzyme H125R, at pH 4.3 and 25°C Pseudomonas putida
1.11.1.19 0.16
-
 H2O2 mutant enzyme E188K/A142V, at pH 5.5 and 25°C Pseudomonas putida
1.11.1.19 0.2
-
 H2O2 mutant enzyme E188K, at pH 4.3 and 25°C Pseudomonas putida
1.11.1.19 0.2
-
 H2O2 mutant enzyme E188K/H125R, at pH 4.3 and 25°C Pseudomonas putida
1.11.1.19 0.22
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme E188K, at pH 4.3 and 25°C Pseudomonas putida
1.11.1.19 0.22
-
 H2O2 mutant enzyme H125R, at pH 4.3 and 25°C Pseudomonas putida
1.11.1.19 0.25
-
 2,6-dimethoxyphenol mutant enzyme A142V, at pH 5.5 and 25°C Pseudomonas putida
1.11.1.19 0.26
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme A142V, at pH 5.5 and 25°C Pseudomonas putida
1.11.1.19 0.28
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme E188K/H125R, at pH 4.3 and 25°C Pseudomonas putida
1.11.1.19 0.5
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme H125R, at pH 4.3 and 25°C Pseudomonas putida
1.11.1.19 1
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme E188K/A142V, at pH 5.5 and 25°C Pseudomonas putida
1.11.1.19 1.1
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme E188K/A142V/H125Y, at pH 5.5 and 25°C Pseudomonas putida
1.11.1.19 2.5
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) wild type enzyme, at pH 4.3 and 25°C Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.19 Pseudomonas putida
-
-
-
1.11.1.19 Pseudomonas putida MET94
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.19 1,2-phenylenediamine + H2O2 + H+
-
 Pseudomonas putida ?
-
 ?
1.11.1.19 1,2-phenylenediamine + H2O2 + H+
-
 Pseudomonas putida MET94 ?
-
 ?
1.11.1.19 1,4-phenylenediamine + H2O2 + H+
-
 Pseudomonas putida ?
-
 ?
1.11.1.19 1,4-phenylenediamine + H2O2 + H+
-
 Pseudomonas putida MET94 ?
-
 ?
1.11.1.19 2 1,2-phenylenediamine + H2O2 + H+
-
 Pseudomonas putida 4a,10a-dihydrophenazine-2,3-diamine + H2O
-
 ?
1.11.1.19 2 2,5-diaminobenzenesulfonic acid + H2O2 + H+
-
 Pseudomonas putida 2,7-diaminophenazine-1,6-disulfonic acid + H2O
-
 ?
1.11.1.19 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2
-
 Pseudomonas putida ?
-
 ?
1.11.1.19 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2
-
 Pseudomonas putida MET94 ?
-
 ?
1.11.1.19 2,5-diaminobenzenesulfonic acid + H2O2 + H+
-
 Pseudomonas putida ?
-
 ?
1.11.1.19 2,6-dimethoxyphenol + H2O2 + H+
-
 Pseudomonas putida ?
-
 ?
1.11.1.19 2,6-dimethoxyphenol + H2O2 + H+
-
 Pseudomonas putida MET94 ?
-
 ?
1.11.1.19 3 1,4-phenylenediamine + H2O2 + H+
-
 Pseudomonas putida (3E,6E)-3,6-bis[(4-aminophenyl)imino]cyclohexa-1,4-diene-1,4-diamine + H2O
-
 ?
1.11.1.19 3 N1-phenylbenzene-1,4-diamine + H2O2 + H+
-
 Pseudomonas putida (3E,6E)-3,6-bis[(4-anilinophenyl)imino]-N1-phenylcyclohexa-1,4-diene-1,4-diamine + H2O
-
 ?
1.11.1.19 4-aminodiphenylamine + H2O2 + H+
-
 Pseudomonas putida ?
-
 ?
1.11.1.19 acetosyringone + H2O2 + H+
-
 Pseudomonas putida ?
-
 ?
1.11.1.19 guaiacol + H2O2 + H+
-
 Pseudomonas putida ?
-
 ?
1.11.1.19 guaiacol + H2O2 + H+
-
 Pseudomonas putida MET94 ?
-
 ?
1.11.1.19 lignin + H2O2 + H+
-
 Pseudomonas putida ?
-
 ?
1.11.1.19 methyl syringate + H2O2 + H+
-
 Pseudomonas putida ?
-
 ?
1.11.1.19 syringaldehyde + H2O2 + H+
-
 Pseudomonas putida ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.19 dye-decolorizing peroxidase
-
 Pseudomonas putida
1.11.1.19 DyP
-
 Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.19 0.02
-
 2,6-dimethoxyphenol mutant enzyme E188K/H125R, at pH 4.3 and 25°C Pseudomonas putida
1.11.1.19 0.03
-
 2,6-dimethoxyphenol mutant enzyme H125R, at pH 4.3 and 25°C Pseudomonas putida
1.11.1.19 0.05
-
 2,6-dimethoxyphenol wild type enzyme, at pH 4.3 and 25°C Pseudomonas putida
1.11.1.19 0.09
-
 2,6-dimethoxyphenol mutant enzyme E188K, at pH 4.3 and 25°C Pseudomonas putida
1.11.1.19 1.8
-
 2,6-dimethoxyphenol mutant enzyme A142V, at pH 5.5 and 25°C Pseudomonas putida
1.11.1.19 3.6
-
 2,6-dimethoxyphenol mutant enzyme E188K/A142V, at pH 5.5 and 25°C Pseudomonas putida
1.11.1.19 4.4
-
 H2O2 mutant enzyme A142V, at pH 5.5 and 25°C Pseudomonas putida
1.11.1.19 4.4
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme A142V, at pH 5.5 and 25°C Pseudomonas putida
1.11.1.19 6
-
 2,6-dimethoxyphenol mutant enzyme E188K/A142V/H125Y, at pH 5.5 and 25°C Pseudomonas putida
1.11.1.19 15
-
 H2O2 mutant enzyme E188K/A142V, at pH 5.5 and 25°C Pseudomonas putida
1.11.1.19 15
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme E188K/A142V, at pH 5.5 and 25°C Pseudomonas putida
1.11.1.19 15
-
 H2O2 mutant enzyme E188K/A142V/H125Y, at pH 5.5 and 25°C Pseudomonas putida
1.11.1.19 15
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme E188K/A142V/H125Y, at pH 5.5 and 25°C Pseudomonas putida
1.11.1.19 23
-
 H2O2 wild type enzyme, at pH 4.3 and 25°C Pseudomonas putida
1.11.1.19 23
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) wild type enzyme, at pH 4.3 and 25°C Pseudomonas putida
1.11.1.19 26
-
 H2O2 mutant enzyme H125R, at pH 4.3 and 25°C Pseudomonas putida
1.11.1.19 26
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme H125R, at pH 4.3 and 25°C Pseudomonas putida
1.11.1.19 69
-
 H2O2 mutant enzyme E188K/H125R, at pH 4.3 and 25°C Pseudomonas putida
1.11.1.19 69
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme E188K/H125R, at pH 4.3 and 25°C Pseudomonas putida
1.11.1.19 128
-
 H2O2 mutant enzyme E188K, at pH 4.3 and 25°C Pseudomonas putida
1.11.1.19 128
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme E188K, at pH 4.3 and 25°C Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.19 4.3
-
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.19 heme
-
 Pseudomonas putida

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.11.1.19 0.7
-
 H2O2 wild type enzyme, at pH 4.3 and 25°C Pseudomonas putida

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.11.1.19 0.16
-
 methyl syringate wild type enzyme, at pH 4.3 and 25°C Pseudomonas putida
1.11.1.19 0.4
-
 2,6-dimethoxyphenol mutant enzyme H125R, at pH 4.3 and 25°C Pseudomonas putida
1.11.1.19 0.45
-
 1,2-phenylenediamine wild type enzyme, at pH 4.3 and 25°C Pseudomonas putida
1.11.1.19 0.6
-
 2,6-dimethoxyphenol mutant enzyme E188K/H125R, at pH 4.3 and 25°C Pseudomonas putida
1.11.1.19 0.7
-
 2,6-dimethoxyphenol wild type enzyme, at pH 4.3 and 25°C Pseudomonas putida
1.11.1.19 1.1
-
 1,2-phenylenediamine mutant enzyme E188K/A142V/H125Y, at pH 8.0 and 25°C Pseudomonas putida
1.11.1.19 1.2
-
 guaiacol wild type enzyme, at pH 4.3 and 25°C Pseudomonas putida
1.11.1.19 1.2
-
 syringaldehyde wild type enzyme, at pH 4.3 and 25°C Pseudomonas putida
1.11.1.19 1.5
-
 2,6-dimethoxyphenol mutant enzyme E188K, at pH 4.3 and 25°C Pseudomonas putida
1.11.1.19 1.6
-
 lignin wild type enzyme, at pH 4.3 and 25°C Pseudomonas putida
1.11.1.19 2.5
-
 1,4-phenylenediamine wild type enzyme, at pH 4.3 and 25°C Pseudomonas putida
1.11.1.19 2.6
-
 acetosyringone wild type enzyme, at pH 4.3 and 25°C Pseudomonas putida
1.11.1.19 2.9
-
 2,5-diaminobenzenesulfonic acid wild type enzyme, at pH 4.3 and 25°C Pseudomonas putida
1.11.1.19 3.8
-
 2,5-diaminobenzenesulfonic acid mutant enzyme E188K/A142V/H125Y, at pH 8.0 and 25°C Pseudomonas putida
1.11.1.19 4.1
-
 lignin mutant enzyme E188K/A142V/H125Y, at pH 8.5 and 25°C Pseudomonas putida
1.11.1.19 5
-
 acetosyringone mutant enzyme E188K/A142V/H125Y, at pH 8.5 and 25°C Pseudomonas putida
1.11.1.19 7.2
-
 2,6-dimethoxyphenol mutant enzyme A142V, at pH 5.5 and 25°C Pseudomonas putida
1.11.1.19 9
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) wild type enzyme, at pH 4.3 and 25°C Pseudomonas putida
1.11.1.19 14
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme E188K/A142V/H125Y, at pH 5.5 and 25°C Pseudomonas putida
1.11.1.19 14
-
 methyl syringate mutant enzyme E188K/A142V/H125Y, at pH 8.0 and 25°C Pseudomonas putida
1.11.1.19 15
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme E188K/A142V, at pH 5.5 and 25°C Pseudomonas putida
1.11.1.19 17
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme A142V, at pH 5.5 and 25°C Pseudomonas putida
1.11.1.19 18
-
 1,4-phenylenediamine mutant enzyme E188K/A142V/H125Y, at pH 8.0 and 25°C Pseudomonas putida
1.11.1.19 22
-
 guaiacol mutant enzyme E188K/A142V/H125Y, at pH 8.5 and 25°C Pseudomonas putida
1.11.1.19 33
-
 syringaldehyde mutant enzyme E188K/A142V/H125Y, at pH 8.5 and 25°C Pseudomonas putida
1.11.1.19 52
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme H125R, at pH 4.3 and 25°C Pseudomonas putida
1.11.1.19 60
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme E188K, at pH 4.3 and 25°C Pseudomonas putida
1.11.1.19 90
-
 H2O2 mutant enzyme A142V, at pH 5.5 and 25°C Pseudomonas putida
1.11.1.19 90
-
 H2O2 mutant enzyme E188K/A142V, at pH 5.5 and 25°C Pseudomonas putida
1.11.1.19 90
-
 2,6-dimethoxyphenol mutant enzyme E188K/A142V, at pH 5.5 and 25°C Pseudomonas putida
1.11.1.19 110
-
 2,6-dimethoxyphenol mutant enzyme E188K/A142V/H125Y, at pH 5.5 and 25°C Pseudomonas putida
1.11.1.19 120
-
 H2O2 mutant enzyme H125R, at pH 4.3 and 25°C Pseudomonas putida
1.11.1.19 220
-
 4-aminodiphenylamine wild type enzyme, at pH 4.3 and 25°C Pseudomonas putida
1.11.1.19 250
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme E188K/H125R, at pH 4.3 and 25°C Pseudomonas putida
1.11.1.19 340
-
 H2O2 mutant enzyme E188K/A142V/H125Y, at pH 5.5 and 25°C Pseudomonas putida
1.11.1.19 350
-
 H2O2 mutant enzyme E188K/H125R, at pH 4.3 and 25°C Pseudomonas putida
1.11.1.19 380
-
 H2O2 wild type enzyme, at pH 4.3 and 25°C Pseudomonas putida
1.11.1.19 420
-
 4-aminodiphenylamine mutant enzyme E188K/A142V/H125Y, at pH 5.0 and 25°C Pseudomonas putida
1.11.1.19 640
-
 H2O2 mutant enzyme E188K, at pH 4.3 and 25°C Pseudomonas putida