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Literature summary extracted from

  • Mendesa, S.; Banhaa, C.; Madeiraa, J.; Santosa, M.; Mirandaa, V.; Manzanerab, M.; Venturaa, M.R.; van Berkel,W.J.H.; Martinsa, L.O.
    Characterization of a bacterial pyranose 2-oxidase from Arthrobactersiccitolerans (2016), J. Mol. Catal. B, 133, s34-S43.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.10 expressed in Escherichia coli BL21 Star (DE3) cells Pseudarthrobacter siccitolerans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.10 0.12
-
 1,4-benzoquinone with D-glucose as cosubstrate, at pH 6.5 and 37°C Pseudarthrobacter siccitolerans
1.1.3.10 1.1
-
 D-glucose with O2 as electron acceptor, at pH 6.5 and 37°C Pseudarthrobacter siccitolerans
1.1.3.10 2.1
-
 D-glucose with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 37°C Pseudarthrobacter siccitolerans
1.1.3.10 14
-
 L-arabinose with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 37°C Pseudarthrobacter siccitolerans
1.1.3.10 73
-
 D-xylose with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 37°C Pseudarthrobacter siccitolerans
1.1.3.10 101
-
 D-galactose with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 37°C Pseudarthrobacter siccitolerans
1.1.3.10 180
-
 D-ribose with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 37°C Pseudarthrobacter siccitolerans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.10 58500
-
-
 Pseudarthrobacter siccitolerans
1.1.3.10 63600
-
 gel filtration Pseudarthrobacter siccitolerans
1.1.3.10 66000
-
 1 * 66000, SDS-PAGE Pseudarthrobacter siccitolerans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.10 D-glucose + O2 Pseudarthrobacter siccitolerans
-
 2-dehydro-D-glucose + H2O2
-
 ?
1.1.3.10 D-glucose + O2 Pseudarthrobacter siccitolerans 4J27
-
 2-dehydro-D-glucose + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.10 Pseudarthrobacter siccitolerans
-
-
-
1.1.3.10 Pseudarthrobacter siccitolerans 4J27
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.10 HisTrap column chromatography Pseudarthrobacter siccitolerans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.10 D-galactose + 1,4-benzoquinone
-
 Pseudarthrobacter siccitolerans 2-dehydro-D-galactose + 1,4-hydroquinone
-
 ?
1.1.3.10 D-galactose + 1,4-benzoquinone
-
 Pseudarthrobacter siccitolerans 4J27 2-dehydro-D-galactose + 1,4-hydroquinone
-
 ?
1.1.3.10 D-galactose + O2
-
 Pseudarthrobacter siccitolerans 2-dehydro-D-galactose + H2O2
-
 ?
1.1.3.10 D-galactose + O2
-
 Pseudarthrobacter siccitolerans 4J27 2-dehydro-D-galactose + H2O2
-
 ?
1.1.3.10 D-glucose + 1,4-benzoquinone
-
 Pseudarthrobacter siccitolerans 2-dehydro-D-glucose + 1,4-hydroquinone
-
 ?
1.1.3.10 D-glucose + O2
-
 Pseudarthrobacter siccitolerans 2-dehydro-D-glucose + H2O2
-
 ?
1.1.3.10 D-glucose + O2 D-glucose is the best substrate Pseudarthrobacter siccitolerans 2-dehydro-D-glucose + H2O2
-
 ?
1.1.3.10 D-glucose + O2
-
 Pseudarthrobacter siccitolerans 4J27 2-dehydro-D-glucose + H2O2
-
 ?
1.1.3.10 D-glucose + O2 D-glucose is the best substrate Pseudarthrobacter siccitolerans 4J27 2-dehydro-D-glucose + H2O2
-
 ?
1.1.3.10 D-ribose + 1,4-benzoquinone
-
 Pseudarthrobacter siccitolerans 2-dehydro-D-ribose + 1,4-hydroquinone
-
 ?
1.1.3.10 D-ribose + O2
-
 Pseudarthrobacter siccitolerans 2-dehydro-D-ribose + H2O2
-
 ?
1.1.3.10 D-xylose + 1,4-benzoquinone
-
 Pseudarthrobacter siccitolerans 2-dehydro-D-xylose + 1,4-hydroquinone
-
 ?
1.1.3.10 D-xylose + O2
-
 Pseudarthrobacter siccitolerans 2-dehydro-D-xylose + H2O2
-
 ?
1.1.3.10 L-arabinose + 1,4-benzoquinone
-
 Pseudarthrobacter siccitolerans 2-dehydro-L-arabinose + 1,4-hydroquinone
-
 ?
1.1.3.10 L-arabinose + O2
-
 Pseudarthrobacter siccitolerans 2-dehydro-L-arabinose + H2O2
-
 ?
1.1.3.10 additional information no activity in the presence of D-mannose, D-fructose, maltose, trehalose, lactose and D-melibiose Pseudarthrobacter siccitolerans ?
-
 ?
1.1.3.10 additional information no activity in the presence of D-mannose, D-fructose, maltose, trehalose, lactose and D-melibiose Pseudarthrobacter siccitolerans 4J27 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.10 monomer 1 * 66000, SDS-PAGE Pseudarthrobacter siccitolerans
1.1.3.10 monomer 1 * 58500, calculated from amino acid sequence Pseudarthrobacter siccitolerans

Synonyms

EC Number Synonyms Comment Organism
1.1.3.10 P2Ox
-
 Pseudarthrobacter siccitolerans
1.1.3.10 pyranose 2-Oxidase
-
 Pseudarthrobacter siccitolerans
1.1.3.10 pyranose:oxygen 2-oxidoreductase
-
 Pseudarthrobacter siccitolerans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.10 37
-
-
 Pseudarthrobacter siccitolerans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.3.10 34 40 more than 50% activity between 34 and 40°C Pseudarthrobacter siccitolerans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.10 40 43 the enzyme shows a melting temperature of 43°C and a half-life at 40°C of 25 min Pseudarthrobacter siccitolerans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.10 0.153
-
 D-glucose with O2 as electron acceptor, at pH 6.5 and 37°C Pseudarthrobacter siccitolerans
1.1.3.10 0.19
-
 L-arabinose with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 37°C Pseudarthrobacter siccitolerans
1.1.3.10 0.38
-
 D-ribose with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 37°C Pseudarthrobacter siccitolerans
1.1.3.10 0.39
-
 1,4-benzoquinone with D-glucose as cosubstrate, at pH 6.5 and 37°C Pseudarthrobacter siccitolerans
1.1.3.10 0.48
-
 D-glucose with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 37°C Pseudarthrobacter siccitolerans
1.1.3.10 0.57
-
 D-galactose with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 37°C Pseudarthrobacter siccitolerans
1.1.3.10 0.91
-
 D-xylose with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 37°C Pseudarthrobacter siccitolerans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.10 6.5
-
-
 Pseudarthrobacter siccitolerans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.3.10 6.5 8 at pH 8.0, the enzyme still retains 45% of its maximal activity observed at pH 6.5 Pseudarthrobacter siccitolerans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.10 FAD
-
 Pseudarthrobacter siccitolerans