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Literature summary extracted from

  • Li, X.; Chatterjee, N.; Spirohn, K.; Boutros, M.; Bohmann, D.
    Cdk12 is a gene-selective RNA polymerase II kinase that regulates a subset of the transcriptome, including Nrf2 target genes (2016), Sci. Rep., 6, 21455.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.23 additional information knockdown of Cdk12 by expression of hairpin RNA Drosophila melanogaster

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.23 nucleus
-
 Drosophila melanogaster 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.23 Mg2+ required Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.23 ATP + [DNA-directed RNA polymerase II] Drosophila melanogaster Cdk12 acts on the Ser2 residue in Pol II CTD heptad repeats ADP + phospho-[DNA-directed RNA polymerase II]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.23 Drosophila melanogaster
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.23 S2 cell
-
 Drosophila melanogaster
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.23 ATP + [DNA-directed RNA polymerase II] Cdk12 acts on the Ser2 residue in Pol II CTD heptad repeats Drosophila melanogaster ADP + phospho-[DNA-directed RNA polymerase II]
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.23 CDK12
-
 Drosophila melanogaster
2.7.11.23 Pol II kinase
-
 Drosophila melanogaster
2.7.11.23 RNA polymerase II kinase
-
 Drosophila melanogaster

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.23 ATP
-
 Drosophila melanogaster

General Information

EC Number General Information Comment Organism
2.7.11.23 malfunction cells lacking CDK12 function are viable and able to proliferate. Antioxidant gene expression is compromised in flies with reduced Cdk12 function, which makes them oxidative stress sensitive, Cdk12 knockdown increases sensitivity to oxidative stress Drosophila melanogaster
2.7.11.23 metabolism the enzyme is involved in the regulation of the Nrf2 signalling pathway Drosophila melanogaster
2.7.11.23 physiological function Cdk12 is a gene-selective RNA polymerase II kinase that regulates a subset of the transcriptome, including Nrf2 target genes. The enzyme engages a global shift in gene expression to switch cells from a metabolically active state to stress-defence mode when challenged by external stress. Cdk12 is not essential for bulk mRNA transcription. Cdk12 may be specifically required for stress activated gene expression. Cdk12 suppresses genes that support metabolic functions in stressed conditions. Cdk12 is a positive regulator of CncC target gene activity. Cdk12 is required for Nrf2 reporter activity in vivo, Cdk12 is critical for the expression of endogenous Nrf2 target genes. Cdk12 promotes stress resistance and overall survival in oxidative stress conditions Drosophila melanogaster