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Literature summary extracted from

  • Shahbaz Mohammadi, H.; Mostafavi, S.S.; Soleimani, S.; Bozorgian, S.; Pooraskari, M.; Kianmehr, A.
    Response surface methodology to optimize partition and purification of two recombinant oxidoreductase enzymes, glucose dehydrogenase and d-galactose dehydrogenase in aqueous two-phase systems (2015), Protein Expr. Purif., 108, 41-47.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.48 expressed in Escherichia coli BL21(DE3) cells Pseudomonas fluorescens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.48 NaCl almost no activity at 10% (w/v) Pseudomonas fluorescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.48 34000
-
 x * 34000, SDS-PAGE Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.48 D-galactose + NAD+ Pseudomonas fluorescens
-
 D-galactono-1,4-lactone + NADH + H+
-
 ?
1.1.1.48 D-galactose + NAD+ Pseudomonas fluorescens AK92
-
 D-galactono-1,4-lactone + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.48 Pseudomonas fluorescens
-
-
-
1.1.1.48 Pseudomonas fluorescens AK92
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.48 aqueous two-phase system composed of 12% (w/v) PEG-6000, 15% (w/v) K2HPO4 with pH 7.5 at 25°C Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.48 D-galactose + NAD+
-
 Pseudomonas fluorescens D-galactono-1,4-lactone + NADH + H+
-
 ?
1.1.1.48 D-galactose + NAD+
-
 Pseudomonas fluorescens AK92 D-galactono-1,4-lactone + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.48 ? x * 34000, SDS-PAGE Pseudomonas fluorescens

Synonyms

EC Number Synonyms Comment Organism
1.1.1.48 D-galactose dehydrogenase
-
 Pseudomonas fluorescens
1.1.1.48 GALDH
-
 Pseudomonas fluorescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.48 7.5
-
-
 Pseudomonas fluorescens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.48 NAD+
-
 Pseudomonas fluorescens