Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Iijima, Y.; Koeduka, T.; Suzuki, H.; Kubota, K.
    Biosynthesis of geranial, a potent aroma compound in ginger rhizome (Zingiber officinale): molecular cloning and characterization of geraniol dehydrogenase (2014), Plant Biotechnol., 31, 525-534.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.183 expressed in Escherichia coli BL21(DE3) cells Zingiber officinale
1.1.1.183 gene ZoGeDH, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, reverse transcription real-time RT-PCR enzyme expression analysis Zingiber officinale

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.183 0.0609
-
 geraniol at pH 9.5 and 25°C Zingiber officinale
1.1.1.183 0.0609
-
 geraniol pH 9.5, 25°C, recombinant enzyme Zingiber officinale
1.1.1.183 0.0926
-
 nerol at pH 9.5 and 25°C Zingiber officinale
1.1.1.183 0.0926
-
 nerol pH 9.5, 25°C, recombinant enzyme Zingiber officinale
1.1.1.183 0.1424
-
 NADP+ at pH 9.5 and 25°C Zingiber officinale
1.1.1.183 0.1424
-
 NADP+ pH 9.5, 25°C, recombinant enzyme Zingiber officinale

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.183 Zn2+ contains zinc Zingiber officinale

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.183 39590
-
-
 Zingiber officinale

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.183 geraniol + NADP+ Zingiber officinale
-
 geranial + NADPH + H+
-
 r
1.1.1.183 geraniol + NADP+ Zingiber officinale 100% activity geranial + NADPH + H+
-
 ?
1.1.1.183 additional information Zingiber officinale feeding exeriments with cell culture: the incorporated geraniol and geranyl acetate are transformed and detected (by GC-MS) as geranial, geraniol, geranyl acetate and citronellol, while nerol and neral are hardly detected ?
-
 ?
1.1.1.183 nerol + NADP+ Zingiber officinale enzyme GeDH shows equal preference for geraniol and nerol neral + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.183 Zingiber officinale
-
-
-
1.1.1.183 Zingiber officinale A0A0E4B3N6 cv. Kintoki
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.183 leaf
-
 Zingiber officinale
-
1.1.1.183 additional information expression levels of ZoGeDH in various ginger plant tissues are in accordance with the accumulation of geranial, except in old rhizome Zingiber officinale
-
1.1.1.183 rhizome
-
 Zingiber officinale
-
1.1.1.183 rhizome young rhizomes Zingiber officinale
-
1.1.1.183 stem
-
 Zingiber officinale
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.183 1-butanol + NADP+ 5.4% activity compared to geraniol Zingiber officinale butanal + NADPH + H+
-
 ?
1.1.1.183 3,7-dimethyloctanol + NADP+ 11.5% activity compared to geraniol Zingiber officinale 3,7-dimethyloctanal + NADPH + H+
-
 ?
1.1.1.183 3-methyl-2-buten-1-ol + NADP+ 22.9% activity compared to geraniol Zingiber officinale 3-methyl-2-buten-1-al + NADPH + H+
-
 ?
1.1.1.183 3-methyl-2-buten-1-ol + NADP+ 22.9% activity compared to geraniol Zingiber officinale 3-methyl-2-butenal + NADPH + H+
-
 ?
1.1.1.183 3-methyl-3-buten-1-ol + NADP+ 6.9% activity compared to geraniol Zingiber officinale 3-methyl-3-buten-1-al + NADPH + H+
-
 ?
1.1.1.183 3-methyl-3-buten-1-ol + NADP+ 6.9% activity compared to geraniol Zingiber officinale 3-methyl-3-butenal + NADPH + H+
-
 ?
1.1.1.183 3-phenylpropanol + NADP+ 16.2% activity compared to geraniol Zingiber officinale 3-phenylpropanal + NADPH + H+
-
 ?
1.1.1.183 cinnamyl alcohol + NADP+ 31.6% activity compared to geraniol Zingiber officinale cinnamaldehyde + NADPH + H+
-
 ?
1.1.1.183 cinnamyl alcohol + NADP+ 31.6% activity compared to geraniol Zingiber officinale cinnamyl aldehyde + NADPH + H+
-
 ?
1.1.1.183 cis-3-hexenol + NADP+ 11.9% activity compared to geraniol Zingiber officinale cis-3-hexenal + NADPH + H+
-
 ?
1.1.1.183 citronellol + NADP+ 16.4% activity compared to geraniol Zingiber officinale citronellal + NADPH + H+
-
 ?
1.1.1.183 geraniol + NADP+
-
 Zingiber officinale geranial + NADPH + H+
-
 r
1.1.1.183 geraniol + NADP+ 100% activity Zingiber officinale geranial + NADPH + H+
-
 ?
1.1.1.183 geraniol + NADP+ enzyme GeDH shows equal preference for geraniol and nerol Zingiber officinale geranial + NADPH + H+
-
 r
1.1.1.183 hexanol + NADP+ 24.1% activity compared to geraniol Zingiber officinale hexanal + NADPH + H+
-
 ?
1.1.1.183 additional information no activity with NAD+ Zingiber officinale ?
-
 ?
1.1.1.183 additional information feeding exeriments with cell culture: the incorporated geraniol and geranyl acetate are transformed and detected (by GC-MS) as geranial, geraniol, geranyl acetate and citronellol, while nerol and neral are hardly detected Zingiber officinale ?
-
 ?
1.1.1.183 additional information the recombinant ZoGeDH catalyzes the NADP+-dependent oxidation from geraniol to citral, a mixture of geranial and neral, but the neral content in vivo is very low. Its substrate specificity is the highest for geraniol and nerol, while that for cinnamyl alcohol is 32% of the activity observed for geraniol Zingiber officinale ?
-
 ?
1.1.1.183 n-butanol + NADP+ 5.4% activity compared to geraniol Zingiber officinale butanal + NADPH + H+
-
 ?
1.1.1.183 nerol + NADP+ 98.3% activity compared to geraniol Zingiber officinale neral + NADPH + H+
-
 r
1.1.1.183 nerol + NADP+ enzyme GeDH shows equal preference for geraniol and nerol Zingiber officinale neral + NADPH + H+
-
 r
1.1.1.183 phenylethylalcohol + NADP+ 9.9% activity compared to geraniol Zingiber officinale phenylethylaldehyde + NADPH + H+
-
 ?
1.1.1.183 trans-2-hexenol + NADP+ 26.6% activity compared to geraniol Zingiber officinale trans-2-hexenal + NADPH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.183 ? x * 39590, sequence calculation Zingiber officinale

Synonyms

EC Number Synonyms Comment Organism
1.1.1.183 GeDH
-
 Zingiber officinale
1.1.1.183 ZoGeDH
-
 Zingiber officinale

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.183 25
-
 assay at Zingiber officinale

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.183 4.6
-
 nerol at pH 9.5 and 25°C Zingiber officinale
1.1.1.183 4.6
-
 nerol pH 9.5, 25°C, recombinant enzyme Zingiber officinale
1.1.1.183 5
-
 NADP+ at pH 9.5 and 25°C Zingiber officinale
1.1.1.183 5
-
 NADP+ pH 9.5, 25°C, recombinant enzyme Zingiber officinale
1.1.1.183 5.4
-
 geraniol at pH 9.5 and 25°C Zingiber officinale
1.1.1.183 5.4
-
 geraniol pH 9.5, 25°C, recombinant enzyme Zingiber officinale

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.183 9.5
-
-
 Zingiber officinale

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.183 8 10 68% of maximal activity is maintained at a pH range of pH 8.0-10.0 Zingiber officinale
1.1.1.183 8 10 68% of the optimum activity is maintained at a pH range of 8.0-10.0 Zingiber officinale

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.183 additional information no activity is observed when NAD+ is substituted for NADP+ Zingiber officinale
1.1.1.183 NADP+
-
 Zingiber officinale
1.1.1.183 NADPH
-
 Zingiber officinale

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.183 Zingiber officinale sequence calculation
-
 6.79

General Information

EC Number General Information Comment Organism
1.1.1.183 evolution phylogenic analysis of ZoGeDH results in its categorization into the cinnamyl alcohol dehydrogenase (CAD) group, along with the previously reported GeDHs of sweet basil (Ocimum basilicum) and wild perilla (P. setoyensis, P. citriodora, and P. frutescens) Zingiber officinale
1.1.1.183 metabolism treatment of plants with D2-geraniol accelerates the synthesis of D1-geraniol and D1-geranial. D1-geranial comprises 33.7% of the endogenous geranial content. Other than geraniol, citronellol is also detected as a mixture of D2- and D1-labeled compounds. On the other hand, nerol and neral, cis-isomers of geraniol and nerol, respectively, are hardly detected in either the control or treated plants. These results indicate that geraniol and geranyl acetate are possible precursors for the formation of geranial and citronellol. Proposed biotransformation of geraniol-related compounds in ginger rhizomes, overview Zingiber officinale

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.183 35
-
 NADP+ at pH 9.5 and 25°C Zingiber officinale
1.1.1.183 35
-
 NADP+ pH 9.5, 25°C, recombinant enzyme Zingiber officinale
1.1.1.183 50
-
 nerol at pH 9.5 and 25°C Zingiber officinale
1.1.1.183 50
-
 nerol pH 9.5, 25°C, recombinant enzyme Zingiber officinale
1.1.1.183 89
-
 geraniol at pH 9.5 and 25°C Zingiber officinale
1.1.1.183 89
-
 geraniol pH 9.5, 25°C, recombinant enzyme Zingiber officinale