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Literature summary extracted from

  • Li, X.C.; Liao, Y.Y.; Leung, D.W.; Wang, H.Y.; Chen, B.L.; Peng, X.X.; Liu, E.E.
    Divergent biochemical and enzymatic properties of oxalate oxidase isoforms encoded by four similar genes in rice (2015), Phytochemistry, 118, 216-223.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.3.4 D-fructose 1,6-bisphosphate
-
 Oryza sativa
1.2.3.4 D-fructose 6-phosphate
-
 Oryza sativa
1.2.3.4 D-glucose 6-phosphate
-
 Oryza sativa

General Stability

EC Number General Stability Organism
1.2.3.4 isoforms OxO1-4 are resistant to a treatment with pepsin at 37°C for 30 min at pH 2.0, while the activity of isoform OxO4 is actually increased on pepsin treatment Oryza sativa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.3.4 CuSO4
-
 Oryza sativa
1.2.3.4 FeSO4
-
 Oryza sativa
1.2.3.4 KNO3
-
 Oryza sativa
1.2.3.4 additional information not influenced by riboflavin and FMN Oryza sativa
1.2.3.4 NaNO3
-
 Oryza sativa
1.2.3.4 NH4NO3
-
 Oryza sativa
1.2.3.4 oxaloacetic acid
-
 Oryza sativa
1.2.3.4 pyruvate
-
 Oryza sativa
1.2.3.4 SDS isoforms OxO1-4, are very sensitive to 0.1% SDS with a nearly total loss of their enzyme activities Oryza sativa
1.2.3.4 ZnSO4
-
 Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.3.4 0.16
-
 oxalate isoform OXO1, at pH 4.0 and 25°C Oryza sativa
1.2.3.4 0.185
-
 oxalate isoform OXO2, at pH 3.5 and 25°C Oryza sativa
1.2.3.4 0.225
-
 oxalate isoform OXO4, at pH 4.0 and 25°C Oryza sativa
1.2.3.4 0.303
-
 oxalate isoform OXO3, at pH 3.0 and 25°C Oryza sativa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.3.4 additional information Na2SO4, EDTA-Na2, KCl, NaCl, K2CO3, Na2CO3, Na2HPO4 and NaH2PO4 have no significant effect on the enzyme activities Oryza sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.3.4 24000
-
-
 Oryza sativa
1.2.3.4 24100
-
-
 Oryza sativa
1.2.3.4 24200
-
-
 Oryza sativa
1.2.3.4 24300
-
-
 Oryza sativa
1.2.3.4 25900
-
-
 Oryza sativa
1.2.3.4 27100
-
-
 Oryza sativa
1.2.3.4 27200
-
-
 Oryza sativa
1.2.3.4 27400
-
-
 Oryza sativa
1.2.3.4 222700
-
 isoform OXO3, gel filtration Oryza sativa
1.2.3.4 235100
-
 isoform OXO1, gel filtration Oryza sativa
1.2.3.4 287000
-
 isoform OXO2, gel filtration Oryza sativa
1.2.3.4 295700
-
 isoform OXO4, gel filtration Oryza sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.3.4 oxalate + O2 + H+ Oryza sativa
-
 CO2 + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.3.4 Oryza sativa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.3.4 Sephadex G-25 gel filtration Oryza sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.3.4 leaf
-
 Oryza sativa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.3.4 additional information no detectable activity against other related organic acids including citrate, glyoxylate, malonate, succinate, glutarate, malate, glycolate, acetate, lactate and formate Oryza sativa ?
-
 ?
1.2.3.4 oxalate + O2 + H+
-
 Oryza sativa CO2 + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.3.4 homodecamer 10 * 24100, isoform OXO1, calculated from amino acid sequence Oryza sativa
1.2.3.4 homodecamer 10 * 24200, isoform OXO4, calculated from amino acid sequence Oryza sativa
1.2.3.4 homodecamer 10 * 24300, isoform OXO2, calculated from amino acid sequence Oryza sativa
1.2.3.4 homodecamer 10 * 25900, isoform OXO1, SDS-PAGE Oryza sativa
1.2.3.4 homodecamer 10 * 27200, isoform OXO2, SDS-PAGE Oryza sativa
1.2.3.4 homodecamer 10 * 27400, isoform OXO4, SDS-PAGE Oryza sativa
1.2.3.4 homooctamer 8 * 24000, isoform OXO3, calculated from amino acid sequence Oryza sativa
1.2.3.4 homooctamer 8 * 27100, isoform OXO3, SDS-PAGE Oryza sativa

Synonyms

EC Number Synonyms Comment Organism
1.2.3.4 OXO1 isoform Oryza sativa
1.2.3.4 OXO2 isoform Oryza sativa
1.2.3.4 OXO3 isoform Oryza sativa
1.2.3.4 OXO4 isoform Oryza sativa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.3.4 70
-
 isoforms OxO2 and OxO3 still maintain all their activities after being heated at 70°C for 1 h while isoforms OxO1 and OxO4 lose about 30% of their activities Oryza sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.3.4 3
-
 isoform OXO3, in succinic acid-NaOH buffer Oryza sativa
1.2.3.4 3.5
-
 isoform OXO2, in succinic acid-NaOH buffer Oryza sativa
1.2.3.4 4
-
 isoforms OXO1 and OXO4, Oryza sativa
1.2.3.4 8.5
-
 isoforms OXO1 and OXO4, in Tris-HCl buffer Oryza sativa

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.2.3.4 1023
-
 isoform OXO1, at pH 4.0 and 25°C Oryza sativa oxalate
1.2.3.4 1504
-
 isoform OXO4, at pH 4.0 and 25°C Oryza sativa oxalate
1.2.3.4 1892
-
 isoform OXO3, at pH 3.0 and 25°C Oryza sativa oxalate
1.2.3.4 2446
-
 isoform OXO2, at pH 3.5 and 25°C Oryza sativa oxalate