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Literature summary extracted from

  • Matsui, D.; Terai, A.; Asano, Y.
    L-Arginine oxidase from Pseudomonas sp. TPU 7192: Characterization, gene cloning, heterologous expression, and application to L-arginine determination (2016), Enzyme Microb. Technol., 82, 151-157.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.25
-
 Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.25 0.149
-
 L-arginine pH 7.0, 37°C Pseudomonas sp.
1.4.3.25 5.25
-
 L-lysine pH 7.0, 37°C Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.25 66000
-
-
 Pseudomonas sp.
1.4.3.25 66300
-
-
 Pseudomonas sp.
1.4.3.25 528000
-
 gel filtration Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.25 Pseudomonas sp. HW613270
-
-
1.4.3.25 Pseudomonas sp. TPU 7192 HW613270
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.25 both native and recombinant protein Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.25 L-arginine + H2O + O2
-
 Pseudomonas sp. 5-guanidino-2-oxopentanoate + NH3 + H2O2 the enzyme catalyzes the oxidative deamination of L-arginine and converts L-arginine to 2-ketoarginine, which is nonenzymatically converted into 4-guanidinobutyric acid when the hydrogen peroxide formed by L-arginine oxidation is not removed ?
1.4.3.25 L-arginine + H2O + O2
-
 Pseudomonas sp. TPU 7192 5-guanidino-2-oxopentanoate + NH3 + H2O2 the enzyme catalyzes the oxidative deamination of L-arginine and converts L-arginine to 2-ketoarginine, which is nonenzymatically converted into 4-guanidinobutyric acid when the hydrogen peroxide formed by L-arginine oxidation is not removed ?
1.4.3.25 L-canavanine + H2O + O2
-
 Pseudomonas sp. 2-oxocanavanine + NH3 + H2O2
-
 ?
1.4.3.25 L-canavanine + H2O + O2
-
 Pseudomonas sp. TPU 7192 2-oxocanavanine + NH3 + H2O2
-
 ?
1.4.3.25 L-homoarginine + H2O + O2
-
 Pseudomonas sp. 2-oxohomoarginine + NH3 + H2O2
-
 ?
1.4.3.25 L-homoarginine + H2O + O2
-
 Pseudomonas sp. TPU 7192 2-oxohomoarginine + NH3 + H2O2
-
 ?
1.4.3.25 L-lysine + H2O + O2
-
 Pseudomonas sp. 2-oxolysine + NH3 + H2O2
-
 ?
1.4.3.25 L-lysine + H2O + O2
-
 Pseudomonas sp. TPU 7192 2-oxolysine + NH3 + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.25 octamer 8 * 66000, SDS-PAGE, 8 * 66300, calculated Pseudomonas sp.

Synonyms

EC Number Synonyms Comment Organism
1.4.3.25 aroD
-
 Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.3.25 55
-
-
 Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.3.25 60
-
 stable up to Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.25 5.5
-
-
 Pseudomonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.3.25 5.5 7.5
-
 Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.25 FAD 1 mol of FAD being contained per mol of subunit. Purified enzyme shows absorption maxima at 275, 375, and 460 nm Pseudomonas sp.